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==Crystal Structure of Unliganded Aspartate Transcarbamoylase from Bacillus subtilis==
==Crystal Structure of Unliganded Aspartate Transcarbamoylase from Bacillus subtilis==
<StructureSection load='3r7d' size='340' side='right' caption='[[3r7d]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
<StructureSection load='3r7d' size='340' side='right'caption='[[3r7d]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[3r7d]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3R7D OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3R7D FirstGlance]. <br>
<table><tr><td colspan='2'>[[3r7d]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3R7D OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3R7D FirstGlance]. <br>
</td></tr><tr><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene><br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.196&#8491;</td></tr>
<tr><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3r7f|3r7f]], [[3r7l|3r7l]]</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
<tr><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">pyrB, BSU15490 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1423 Bacillus subtilis])</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3r7d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3r7d OCA], [https://pdbe.org/3r7d PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3r7d RCSB], [https://www.ebi.ac.uk/pdbsum/3r7d PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3r7d ProSAT]</span></td></tr>
<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Aspartate_carbamoyltransferase Aspartate carbamoyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.1.3.2 2.1.3.2] </span></td></tr>
</table>
<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3r7d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3r7d OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3r7d RCSB], [http://www.ebi.ac.uk/pdbsum/3r7d PDBsum]</span></td></tr>
== Function ==
<table>
[https://www.uniprot.org/uniprot/PYRB_BACSU PYRB_BACSU]
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Here, we report high-resolution X-ray structures of Bacillus subtilis aspartate transcarbamoylase (ATCase), an enzyme that catalyzes one of the first reactions in pyrimidine nucleotide biosynthesis. Structures of the enzyme have been determined in the absence of ligands, in the presence of the substrate carbamoyl phosphate, and in the presence of the bisubstrate/transition state analog N-phosphonacetyl-l-aspartate. Combining the structural data with in silico docking and electrostatic calculations, we have been able to visualize each step in the catalytic cycle of ATCase, from the ordered binding of the substrates, to the formation and decomposition of the tetrahedral intermediate, to the ordered release of the products from the active site. Analysis of the conformational changes associated with these steps provides a rationale for the lack of cooperativity in trimeric ATCases that do not possess regulatory subunits.
 
Crystallographic Snapshots of the Complete Catalytic Cycle of the Unregulated Aspartate Transcarbamoylase from Bacillus subtilis.,Harris KM, Cockrell GM, Puleo DE, Kantrowitz ER J Mol Biol. 2011 May 31. PMID:21663747<ref>PMID:21663747</ref>


From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
==See Also==
</div>
*[[Aspartate carbamoyltransferase 3D structures|Aspartate carbamoyltransferase 3D structures]]
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Aspartate carbamoyltransferase]]
[[Category: Bacillus subtilis]]
[[Category: Bacillus subtilis]]
[[Category: Cockrell, G M.]]
[[Category: Large Structures]]
[[Category: Harris, K M.]]
[[Category: Cockrell GM]]
[[Category: Kantrowitz, E R.]]
[[Category: Harris KM]]
[[Category: Puleo, D E.]]
[[Category: Kantrowitz ER]]
[[Category: Aspartate transcarbamoylase]]
[[Category: Puleo DE]]
[[Category: Catalytic cycle]]
[[Category: Transferase]]

Latest revision as of 13:52, 21 February 2024

Crystal Structure of Unliganded Aspartate Transcarbamoylase from Bacillus subtilisCrystal Structure of Unliganded Aspartate Transcarbamoylase from Bacillus subtilis

Structural highlights

3r7d is a 3 chain structure with sequence from Bacillus subtilis. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 2.196Å
Ligands:
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

PYRB_BACSU

See Also

3r7d, resolution 2.20Å

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