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==The 2.3 A structure of porcine circovirus 2==
==The 2.3 A structure of porcine circovirus 2==
<StructureSection load='3r0r' size='340' side='right' caption='[[3r0r]], [[Resolution|resolution]] 2.35&Aring;' scene=''>
<StructureSection load='3r0r' size='340' side='right'caption='[[3r0r]], [[Resolution|resolution]] 2.35&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[3r0r]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Pcv2 Pcv2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3R0R OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3R0R FirstGlance]. <br>
<table><tr><td colspan='2'>[[3r0r]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Porcine_circovirus_2 Porcine circovirus 2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3R0R OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3R0R FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.35&#8491;</td></tr>
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">cap, Cap, ORF2, cp ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=85708 PCV2])</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3r0r FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3r0r OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3r0r RCSB], [http://www.ebi.ac.uk/pdbsum/3r0r PDBsum]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3r0r FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3r0r OCA], [https://pdbe.org/3r0r PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3r0r RCSB], [https://www.ebi.ac.uk/pdbsum/3r0r PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3r0r ProSAT]</span></td></tr>
</table>
</table>
<div style="background-color:#fffaf0;">
== Function ==
== Publication Abstract from PubMed ==
[https://www.uniprot.org/uniprot/Q805N7_PCV2 Q805N7_PCV2]
Porcine circovirus 2 (PCV2) is a T=1 non-enveloped icosahedral virus that has had severe impact on the swine industry. Here we report the crystal structure of an N-terminally truncated PCV2 virus-like particle at 2.3 A resolution, and the cryo-EM image reconstruction of a full length PCV2 virus-like particle at 9.6 A resolution. This is the first atomic structure of a Circovirus. The crystal structure revealed that the capsid protein fold is a canonical viral jelly-roll. The loops connecting the strands of the jelly-roll define the limited features of the surface. Sulfate ions interacting with the surface, and electrostatic potential calculations strongly suggest a heparan sulfate binding site that allows PCV2 to gain entry into the cell. The crystal structure also allowed previously determined epitopes of the capsid to be visualized. The cryo-EM image reconstruction showed that the location of the N-terminus, absent in the crystal structure, is inside the capsid. As the N-terminus was previously shown to be antigenic it may externalize through viral 'breathing'.
 
The 2.3 A Structure of Porcine Circovirus 2.,Khayat R, Brunn N, Speir JA, Hardham JM, Ankenbauer RG, Schneemann A, Johnson JE J Virol. 2011 Jun 1. PMID:21632760<ref>PMID:21632760</ref>
 
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>


==See Also==
==See Also==
*[[Virus coat protein|Virus coat protein]]
*[[Virus coat proteins 3D structures|Virus coat proteins 3D structures]]
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Pcv2]]
[[Category: Large Structures]]
[[Category: Johnson, J E]]
[[Category: Porcine circovirus 2]]
[[Category: Khayat, R]]
[[Category: Johnson JE]]
[[Category: Speir, J A]]
[[Category: Khayat R]]
[[Category: Beta barrel]]
[[Category: Speir JA]]
[[Category: Ssdna]]
[[Category: Viral jelly roll]]
[[Category: Virus]]

Latest revision as of 13:51, 21 February 2024

The 2.3 A structure of porcine circovirus 2The 2.3 A structure of porcine circovirus 2

Structural highlights

3r0r is a 1 chain structure with sequence from Porcine circovirus 2. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 2.35Å
Ligands:
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

Q805N7_PCV2

See Also

3r0r, resolution 2.35Å

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