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==Crystal Structure of a vesicular stomatitis virus nucleocapsid-polyC complex==
==Crystal Structure of a vesicular stomatitis virus nucleocapsid-polyC complex==
<StructureSection load='3pu0' size='340' side='right' caption='[[3pu0]], [[Resolution|resolution]] 3.09&Aring;' scene=''>
<StructureSection load='3pu0' size='340' side='right'caption='[[3pu0]], [[Resolution|resolution]] 3.09&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[3pu0]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Vesicular_stomatitis_indiana_virus Vesicular stomatitis indiana virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3PU0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3PU0 FirstGlance]. <br>
<table><tr><td colspan='2'>[[3pu0]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Vesicular_stomatitis_Indiana_virus Vesicular stomatitis Indiana virus] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3PU0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3PU0 FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=IUM:URANYL+(VI)+ION'>IUM</scene></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.09&#8491;</td></tr>
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3pto|3pto]], [[3ptx|3ptx]], [[3pu1|3pu1]], [[3pu4|3pu4]]</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=IUM:URANYL+(VI)+ION'>IUM</scene></td></tr>
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">N ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=11277 Vesicular stomatitis Indiana virus])</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3pu0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3pu0 OCA], [https://pdbe.org/3pu0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3pu0 RCSB], [https://www.ebi.ac.uk/pdbsum/3pu0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3pu0 ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3pu0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3pu0 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3pu0 RCSB], [http://www.ebi.ac.uk/pdbsum/3pu0 PDBsum]</span></td></tr>
</table>
</table>
<div style="background-color:#fffaf0;">
== Function ==
== Publication Abstract from PubMed ==
[https://www.uniprot.org/uniprot/NCAP_VSIVA NCAP_VSIVA] Encapsidates the genome in a ratio of one N per nine ribonucleotides, protecting it from nucleases. The encapsidated genomic RNA is termed the NC and serves as template for transcription and replication. Replication is dependent on intracellular concentration of newly synthesized N, termed N(0), which corresponds to the protein not associated with RNA. In contrast, when associated with RNA, it is termed N. During replication, encapsidation by N(0) is coupled to RNA synthesis and all replicative products are resistant to nucleases (By similarity).
The genomic RNA of negative strand RNA viruses, such as vesicular stomatitis virus (VSV), is completely enwrapped by the nucleocapsid protein (N) in every stage of virus infection. During viral transcription/replication, however, the genomic RNA in the nucleocapsid must be accessible by the virus-encoded RNA-dependent RNA polymerase in order to serve as the template for RNA synthesis. With the VSV nucleocapsid and a nucleocapsid-like particle (NLP) produced in E. coli, we have found that the RNA in the VSV nucleocapsid can be removed by RNase A, in contrast to what was previously reported. Removal of the RNA did not disrupt the assembly of the N protein, resulting in an empty capsid. Poly-ribonucleotides could be re-encapsidated into the empty NLP and the crystal structures were determined. The crystal structures revealed variable degrees of association of the N protein with a specific RNA sequence.
 
Access of RNA encapsidated in the nucleocapsid of vesicular stomatitis virus.,Green TJ, Rowse M, Tsao J, Kang J, Ge P, Zhou ZH, Luo M J Virol. 2010 Dec 22. PMID:21177817<ref>PMID:21177817</ref>
 
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>


==See Also==
==See Also==
*[[Nucleoprotein|Nucleoprotein]]
*[[Nucleoprotein 3D structures|Nucleoprotein 3D structures]]
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Vesicular stomatitis indiana virus]]
[[Category: Large Structures]]
[[Category: Green, T J]]
[[Category: Synthetic construct]]
[[Category: Luo, M]]
[[Category: Vesicular stomatitis Indiana virus]]
[[Category: Rowse, M]]
[[Category: Green TJ]]
[[Category: Nucleocapsid]]
[[Category: Luo M]]
[[Category: Rna]]
[[Category: Rowse M]]
[[Category: Viral protein-rna complex]]

Latest revision as of 13:43, 21 February 2024

Crystal Structure of a vesicular stomatitis virus nucleocapsid-polyC complexCrystal Structure of a vesicular stomatitis virus nucleocapsid-polyC complex

Structural highlights

3pu0 is a 6 chain structure with sequence from Vesicular stomatitis Indiana virus and Synthetic construct. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 3.09Å
Ligands:
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

NCAP_VSIVA Encapsidates the genome in a ratio of one N per nine ribonucleotides, protecting it from nucleases. The encapsidated genomic RNA is termed the NC and serves as template for transcription and replication. Replication is dependent on intracellular concentration of newly synthesized N, termed N(0), which corresponds to the protein not associated with RNA. In contrast, when associated with RNA, it is termed N. During replication, encapsidation by N(0) is coupled to RNA synthesis and all replicative products are resistant to nucleases (By similarity).

See Also

3pu0, resolution 3.09Å

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