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==The structure of the CRISPR-associated protein, csa2, from Sulfolobus solfataricus==
==The structure of the CRISPR-associated protein, csa2, from Sulfolobus solfataricus==
<StructureSection load='3ps0' size='340' side='right' caption='[[3ps0]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
<StructureSection load='3ps0' size='340' side='right'caption='[[3ps0]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[3ps0]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Sulfolobus_solfataricus Sulfolobus solfataricus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3PS0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3PS0 FirstGlance]. <br>
<table><tr><td colspan='2'>[[3ps0]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharolobus_solfataricus_P2 Saccharolobus solfataricus P2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3PS0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3PS0 FirstGlance]. <br>
</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">SSO1442 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=2287 Sulfolobus solfataricus])</td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ps0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ps0 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3ps0 RCSB], [http://www.ebi.ac.uk/pdbsum/3ps0 PDBsum]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3ps0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ps0 OCA], [https://pdbe.org/3ps0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3ps0 RCSB], [https://www.ebi.ac.uk/pdbsum/3ps0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3ps0 ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
[[http://www.uniprot.org/uniprot/Q97Y91_SULSO Q97Y91_SULSO]] CRISPR (clustered regularly interspaced short palindromic repeat) is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain spacers, sequences complementary to antecedent mobile elements, and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA) (By similarity).  
[https://www.uniprot.org/uniprot/CSA2B_SACS2 CSA2B_SACS2] CRISPR (clustered regularly interspaced short palindromic repeat) is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain spacers, sequences complementary to antecedent mobile elements, and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA) (By similarity).
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
In response to viral infection, many prokaryotes incorporate fragments of virus-derived DNA into loci called clustered regularly interspaced short palindromic repeats (CRISPRs). The loci are then transcribed, and the processed CRISPR transcripts are used to target invading viral DNA and RNA. The Escherichia coli "CRISPR-associated complex for antiviral defense" (CASCADE) is central in targeting invading DNA. Here we report the structural and functional characterization of an archaeal CASCADE (aCASCADE) from Sulfolobus solfataricus. Tagged Csa2 (Cas7) expressed in S. solfataricus co-purifies with Cas5a-, Cas6-, Csa5-, and Cas6-processed CRISPR-RNA (crRNA). Csa2, the dominant protein in aCASCADE, forms a stable complex with Cas5a. Transmission electron microscopy reveals a helical complex of variable length, perhaps due to substoichiometric amounts of other CASCADE components. A recombinant Csa2-Cas5a complex is sufficient to bind crRNA and complementary ssDNA. The structure of Csa2 reveals a crescent-shaped structure unexpectedly composed of a modified RNA-recognition motif and two additional domains present as insertions in the RNA-recognition motif. Conserved residues indicate potential crRNA- and target DNA-binding sites, and the H160A variant shows significantly reduced affinity for crRNA. We propose a general subunit architecture for CASCADE in other bacteria and Archaea.
 
Structural and functional characterization of an archaeal clustered regularly interspaced short palindromic repeat (CRISPR)-associated complex for antiviral defense (CASCADE).,Lintner NG, Kerou M, Brumfield SK, Graham S, Liu H, Naismith JH, Sdano M, Peng N, She Q, Copie V, Young MJ, White MF, Lawrence CM J Biol Chem. 2011 Jun 17;286(24):21643-56. Epub 2011 Apr 20. PMID:21507944<ref>PMID:21507944</ref>
 
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>


==See Also==
==See Also==
*[[Endonuclease|Endonuclease]]
*[[CRISPR-Cas|CRISPR-Cas]]
== References ==
*[[Endonuclease 3D structures|Endonuclease 3D structures]]
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Sulfolobus solfataricus]]
[[Category: Large Structures]]
[[Category: Lawrence, C M]]
[[Category: Saccharolobus solfataricus P2]]
[[Category: Lintner, N G]]
[[Category: Lawrence CM]]
[[Category: Sdano, M]]
[[Category: Lintner NG]]
[[Category: Young, M J]]
[[Category: Sdano M]]
[[Category: Ca]]
[[Category: Young MJ]]
[[Category: Cass]]
[[Category: Crispr]]
[[Category: Crispr-associated]]
[[Category: Nucleic-acid binding]]
[[Category: Protein-protein interaction]]
[[Category: Rna binding protein]]
[[Category: Rna-binding]]
[[Category: Rna-binding protein]]
[[Category: Rna-recognition motif]]
[[Category: Viral resistance]]

Latest revision as of 13:43, 21 February 2024

The structure of the CRISPR-associated protein, csa2, from Sulfolobus solfataricusThe structure of the CRISPR-associated protein, csa2, from Sulfolobus solfataricus

Structural highlights

3ps0 is a 4 chain structure with sequence from Saccharolobus solfataricus P2. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 2Å
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

CSA2B_SACS2 CRISPR (clustered regularly interspaced short palindromic repeat) is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain spacers, sequences complementary to antecedent mobile elements, and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA) (By similarity).

See Also

3ps0, resolution 2.00Å

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