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==Crystal structure of geranylgeranyl pyrophosphate synthase from lactobacillus brevis atcc 367 complexed with citrate==
==Crystal structure of geranylgeranyl pyrophosphate synthase from lactobacillus brevis atcc 367 complexed with citrate==
<StructureSection load='3pko' size='340' side='right' caption='[[3pko]], [[Resolution|resolution]] 1.98&Aring;' scene=''>
<StructureSection load='3pko' size='340' side='right'caption='[[3pko]], [[Resolution|resolution]] 1.98&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[3pko]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Lacba Lacba]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3PKO OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3PKO FirstGlance]. <br>
<table><tr><td colspan='2'>[[3pko]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Levilactobacillus_brevis_ATCC_367 Levilactobacillus brevis ATCC 367]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3PKO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3PKO FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CIT:CITRIC+ACID'>CIT</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.98&#8491;</td></tr>
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3n3d|3n3d]]</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CIT:CITRIC+ACID'>CIT</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">LVIS_1638 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=387344 LACBA])</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3pko FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3pko OCA], [https://pdbe.org/3pko PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3pko RCSB], [https://www.ebi.ac.uk/pdbsum/3pko PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3pko ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3pko FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3pko OCA], [http://pdbe.org/3pko PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3pko RCSB], [http://www.ebi.ac.uk/pdbsum/3pko PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3pko ProSAT]</span></td></tr>
</table>
</table>
<div style="background-color:#fffaf0;">
== Function ==
== Publication Abstract from PubMed ==
[https://www.uniprot.org/uniprot/Q03Q08_LEVBA Q03Q08_LEVBA]
The number of available protein sequences has increased exponentially with the advent of high-throughput genomic sequencing, creating a significant challenge for functional annotation. Here, we describe a large-scale study on assigning function to unknown members of the trans-polyprenyl transferase (E-PTS) subgroup in the isoprenoid synthase superfamily, which provides substrates for the biosynthesis of the more than 55,000 isoprenoid metabolites. Although the mechanism for determining the product chain length for these enzymes is known, there is no simple relationship between function and primary sequence, so that assigning function is challenging. We addressed this challenge through large-scale bioinformatics analysis of &gt;5,000 putative polyprenyl transferases; experimental characterization of the chain-length specificity of 79 diverse members of this group; determination of 27 structures of 19 of these enzymes, including seven cocrystallized with substrate analogs or products; and the development and successful application of a computational approach to predict function that leverages available structural data through homology modeling and docking of possible products into the active site. The crystallographic structures and computational structural models of the enzyme-ligand complexes elucidate the structural basis of specificity. As a result of this study, the percentage of E-PTS sequences similar to functionally annotated ones (BLAST e-value &lt;/= 1e-70) increased from 40.6 to 68.8%, and the percentage of sequences similar to available crystal structures increased from 28.9 to 47.4%. The high accuracy of our blind prediction of newly characterized enzymes indicates the potential to predict function to the complete polyprenyl transferase subgroup of the isoprenoid synthase superfamily computationally.
 
Prediction of function for the polyprenyl transferase subgroup in the isoprenoid synthase superfamily.,Wallrapp FH, Pan JJ, Ramamoorthy G, Almonacid DE, Hillerich BS, Seidel R, Patskovsky Y, Babbitt PC, Almo SC, Jacobson MP, Poulter CD Proc Natl Acad Sci U S A. 2013 Mar 14. PMID:23493556<ref>PMID:23493556</ref>
 
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 3pko" style="background-color:#fffaf0;"></div>


==See Also==
==See Also==
*[[Geranylgeranyl pyrophosphate synthase|Geranylgeranyl pyrophosphate synthase]]
*[[Geranylgeranyl pyrophosphate synthase 3D structures|Geranylgeranyl pyrophosphate synthase 3D structures]]
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Lacba]]
[[Category: Large Structures]]
[[Category: Almo, S C]]
[[Category: Levilactobacillus brevis ATCC 367]]
[[Category: Burley, S K]]
[[Category: Almo SC]]
[[Category: Chang, S]]
[[Category: Burley SK]]
[[Category: Gerlt, J A]]
[[Category: Chang S]]
[[Category: Structural genomic]]
[[Category: Gerlt JA]]
[[Category: Patskovsky, Y]]
[[Category: Patskovsky Y]]
[[Category: Poulter, C D]]
[[Category: Poulter CD]]
[[Category: Rutter, M]]
[[Category: Rutter M]]
[[Category: Sauder, J M]]
[[Category: Sauder JM]]
[[Category: Toro, R]]
[[Category: Toro R]]
[[Category: Isoprenyl diphosphate synthase]]
[[Category: NYSGXRC, New York SGX Research Center for Structural Genomics]]
[[Category: Nysgrc]]
[[Category: PSI, Protein structure initiative]]
[[Category: Transferase]]

Latest revision as of 13:41, 21 February 2024

Crystal structure of geranylgeranyl pyrophosphate synthase from lactobacillus brevis atcc 367 complexed with citrateCrystal structure of geranylgeranyl pyrophosphate synthase from lactobacillus brevis atcc 367 complexed with citrate

Structural highlights

3pko is a 2 chain structure with sequence from Levilactobacillus brevis ATCC 367. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 1.98Å
Ligands:,
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

Q03Q08_LEVBA

See Also

3pko, resolution 1.98Å

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