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| ==Precursor bound NifEN== | | ==Precursor bound NifEN== |
| <StructureSection load='3pdi' size='340' side='right' caption='[[3pdi]], [[Resolution|resolution]] 2.40Å' scene=''> | | <StructureSection load='3pdi' size='340' side='right'caption='[[3pdi]], [[Resolution|resolution]] 2.40Å' scene=''> |
| == Structural highlights == | | == Structural highlights == |
| <table><tr><td colspan='2'>[[3pdi]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/Azovd Azovd]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3PDI OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3PDI FirstGlance]. <br> | | <table><tr><td colspan='2'>[[3pdi]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Azotobacter_vinelandii_DJ Azotobacter vinelandii DJ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3PDI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3PDI FirstGlance]. <br> |
| </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CZL:L-CLUSTER+(FE8S9)'>CZL</scene>, <scene name='pdbligand=SF4:IRON/SULFUR+CLUSTER'>SF4</scene></td></tr> | | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.4Å</td></tr> |
| <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Avin_01450, nifE ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=322710 AZOVD]), Avin_01470, nifN ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=322710 AZOVD])</td></tr> | | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CZL:L-CLUSTER+(FE8S9)'>CZL</scene>, <scene name='pdbligand=SF4:IRON/SULFUR+CLUSTER'>SF4</scene></td></tr> |
| <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3pdi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3pdi OCA], [http://pdbe.org/3pdi PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3pdi RCSB], [http://www.ebi.ac.uk/pdbsum/3pdi PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3pdi ProSAT]</span></td></tr> | | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3pdi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3pdi OCA], [https://pdbe.org/3pdi PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3pdi RCSB], [https://www.ebi.ac.uk/pdbsum/3pdi PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3pdi ProSAT]</span></td></tr> |
| </table> | | </table> |
| <div style="background-color:#fffaf0;">
| | == Function == |
| == Publication Abstract from PubMed == | | [https://www.uniprot.org/uniprot/C1DH03_AZOVD C1DH03_AZOVD] |
| NifEN plays an essential role in the biosynthesis of the nitrogenase iron-molybdenum (FeMo) cofactor (M cluster). It is an alpha(2)beta(2) tetramer that is homologous to the catalytic molybdenum-iron (MoFe) protein (NifDK) component of nitrogenase. NifEN serves as a scaffold for the conversion of an iron-only precursor to a matured form of the M cluster before delivering the latter to its target location within NifDK. Here, we present the structure of the precursor-bound NifEN of Azotobacter vinelandii at 2.6 angstrom resolution. From a structural comparison of NifEN with des-M-cluster NifDK and holo NifDK, we propose similar pathways of cluster insertion for the homologous NifEN and NifDK proteins.
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| Structure of precursor-bound NifEN: a nitrogenase FeMo cofactor maturase/insertase.,Kaiser JT, Hu Y, Wiig JA, Rees DC, Ribbe MW Science. 2011 Jan 7;331(6013):91-4. PMID:21212358<ref>PMID:21212358</ref>
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| From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
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| </div>
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| <div class="pdbe-citations 3pdi" style="background-color:#fffaf0;"></div>
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| ==See Also==
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| *[[Nitrogenase|Nitrogenase]]
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| == References ==
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| <references/>
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| __TOC__ | | __TOC__ |
| </StructureSection> | | </StructureSection> |
| [[Category: Azovd]] | | [[Category: Azotobacter vinelandii DJ]] |
| [[Category: Hu, Y]] | | [[Category: Large Structures]] |
| [[Category: Kaiser, J T]] | | [[Category: Hu Y]] |
| [[Category: Rees, D C]] | | [[Category: Kaiser JT]] |
| [[Category: Ribbe, M W]] | | [[Category: Rees DC]] |
| [[Category: Wiig, J A]] | | [[Category: Ribbe MW]] |
| [[Category: Nifb]] | | [[Category: Wiig JA]] |
| [[Category: Nifdk]]
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| [[Category: Nifh]]
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| [[Category: Nitrogenase cofactor maturation]]
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| [[Category: Protein binding]]
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