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==Precursor bound NifEN==
==Precursor bound NifEN==
<StructureSection load='3pdi' size='340' side='right' caption='[[3pdi]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
<StructureSection load='3pdi' size='340' side='right'caption='[[3pdi]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[3pdi]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/Azotobacter_vinelandii Azotobacter vinelandii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3PDI OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3PDI FirstGlance]. <br>
<table><tr><td colspan='2'>[[3pdi]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Azotobacter_vinelandii_DJ Azotobacter vinelandii DJ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3PDI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3PDI FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CZL:L-CLUSTER+(FE8S9)'>CZL</scene>, <scene name='pdbligand=SF4:IRON/SULFUR+CLUSTER'>SF4</scene></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.4&#8491;</td></tr>
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Avin_01450, nifE ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=354 Azotobacter vinelandii]), Avin_01470, nifN ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=354 Azotobacter vinelandii])</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CZL:L-CLUSTER+(FE8S9)'>CZL</scene>, <scene name='pdbligand=SF4:IRON/SULFUR+CLUSTER'>SF4</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3pdi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3pdi OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3pdi RCSB], [http://www.ebi.ac.uk/pdbsum/3pdi PDBsum]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3pdi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3pdi OCA], [https://pdbe.org/3pdi PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3pdi RCSB], [https://www.ebi.ac.uk/pdbsum/3pdi PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3pdi ProSAT]</span></td></tr>
</table>
</table>
<div style="background-color:#fffaf0;">
== Function ==
== Publication Abstract from PubMed ==
[https://www.uniprot.org/uniprot/C1DH03_AZOVD C1DH03_AZOVD]  
NifEN plays an essential role in the biosynthesis of the nitrogenase iron-molybdenum (FeMo) cofactor (M cluster). It is an alpha(2)beta(2) tetramer that is homologous to the catalytic molybdenum-iron (MoFe) protein (NifDK) component of nitrogenase. NifEN serves as a scaffold for the conversion of an iron-only precursor to a matured form of the M cluster before delivering the latter to its target location within NifDK. Here, we present the structure of the precursor-bound NifEN of Azotobacter vinelandii at 2.6 angstrom resolution. From a structural comparison of NifEN with des-M-cluster NifDK and holo NifDK, we propose similar pathways of cluster insertion for the homologous NifEN and NifDK proteins.
 
Structure of precursor-bound NifEN: a nitrogenase FeMo cofactor maturase/insertase.,Kaiser JT, Hu Y, Wiig JA, Rees DC, Ribbe MW Science. 2011 Jan 7;331(6013):91-4. PMID:21212358<ref>PMID:21212358</ref>
 
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
 
==See Also==
*[[Nitrogenase|Nitrogenase]]
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Azotobacter vinelandii]]
[[Category: Azotobacter vinelandii DJ]]
[[Category: Hu, Y]]
[[Category: Large Structures]]
[[Category: Kaiser, J T]]
[[Category: Hu Y]]
[[Category: Rees, D C]]
[[Category: Kaiser JT]]
[[Category: Ribbe, M W]]
[[Category: Rees DC]]
[[Category: Wiig, J A]]
[[Category: Ribbe MW]]
[[Category: Nifb]]
[[Category: Wiig JA]]
[[Category: Nifdk]]
[[Category: Nifh]]
[[Category: Nitrogenase cofactor maturation]]
[[Category: Protein binding]]

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