3oy7: Difference between revisions

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==Crystal structure of a virus encoded glycosyltransferase in complex with GDP-mannose==
==Crystal structure of a virus encoded glycosyltransferase in complex with GDP-mannose==
<StructureSection load='3oy7' size='340' side='right' caption='[[3oy7]], [[Resolution|resolution]] 2.73&Aring;' scene=''>
<StructureSection load='3oy7' size='340' side='right'caption='[[3oy7]], [[Resolution|resolution]] 2.73&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[3oy7]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Paramecium_bursaria_chlorella_virus_ny2a Paramecium bursaria chlorella virus ny2a]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3OY7 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3OY7 FirstGlance]. <br>
<table><tr><td colspan='2'>[[3oy7]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Paramecium_bursaria_Chlorella_virus_NY2A Paramecium bursaria Chlorella virus NY2A]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3OY7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3OY7 FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GDD:GUANOSINE-5-DIPHOSPHATE-ALPHA-D-MANNOSE'>GDD</scene></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.73&#8491;</td></tr>
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3oy2|3oy2]]</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GDD:GUANOSINE-5-DIPHOSPHATE-ALPHA-D-MANNOSE'>GDD</scene></td></tr>
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">B736L, NY2A_B736L ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=46021 Paramecium bursaria Chlorella virus NY2A])</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3oy7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3oy7 OCA], [https://pdbe.org/3oy7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3oy7 RCSB], [https://www.ebi.ac.uk/pdbsum/3oy7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3oy7 ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3oy7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3oy7 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3oy7 RCSB], [http://www.ebi.ac.uk/pdbsum/3oy7 PDBsum]</span></td></tr>
</table>
</table>
<div style="background-color:#fffaf0;">
== Function ==
== Publication Abstract from PubMed ==
[https://www.uniprot.org/uniprot/A7IXR1_PBCVN A7IXR1_PBCVN]
The chloroviruses (family Phycodnaviridae), unlike most viruses, encode some, if not most, of the enzymes involved in the glycosylation of their structural proteins. Annotation of the gene product B736L from chlorovirus NY-2A suggests that it is a glycosyltransferase. The structure of the recombinantly expressed B736L protein was determined by X-ray crystallography to 2.3-A resolution, and the protein was shown to have two nucleotide-binding folds like other glycosyltransferase type B enzymes. This is the second structure of a chlorovirus-encoded glycosyltransferase and the first structure of a chlorovirus type B enzyme to be determined. B736L is a retaining enzyme and belongs to glycosyltransferase family 4. The donor substrate was identified as GDP-mannose by isothermal titration calorimetry and was shown to bind into the cleft between the two domains in the protein. The active form of the enzyme is probably a dimer in which the active centers are separated by about 40 A.
 
Crystal structure of a virus-encoded putative glycosyltransferase.,Xiang Y, Baxa U, Zhang Y, Steven AC, Lewis GL, Van Etten JL, Rossmann MG J Virol. 2010 Dec;84(23):12265-73. Epub 2010 Sep 22. PMID:20861263<ref>PMID:20861263</ref>
 
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>


==See Also==
==See Also==
*[[Glycosyltransferase|Glycosyltransferase]]
*[[Glycosyltransferase 3D structures|Glycosyltransferase 3D structures]]
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Paramecium bursaria chlorella virus ny2a]]
[[Category: Large Structures]]
[[Category: Rossmann, M G]]
[[Category: Paramecium bursaria Chlorella virus NY2A]]
[[Category: Xiang, Y]]
[[Category: Rossmann MG]]
[[Category: Gdp-mannose]]
[[Category: Xiang Y]]
[[Category: Glycosyltransferase]]
[[Category: Rossmann fold]]
[[Category: Sugar]]
[[Category: Transferase]]
[[Category: Viral protein]]
[[Category: Virus surface]]

Latest revision as of 13:37, 21 February 2024

Crystal structure of a virus encoded glycosyltransferase in complex with GDP-mannoseCrystal structure of a virus encoded glycosyltransferase in complex with GDP-mannose

Structural highlights

3oy7 is a 2 chain structure with sequence from Paramecium bursaria Chlorella virus NY2A. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 2.73Å
Ligands:
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

A7IXR1_PBCVN

See Also

3oy7, resolution 2.73Å

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