3nk5: Difference between revisions

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[[Image:3nk5.jpg|left|200px]]


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==Crystal structure of AqpZ mutant F43W==
The line below this paragraph, containing "STRUCTURE_3nk5", creates the "Structure Box" on the page.
<StructureSection load='3nk5' size='340' side='right'caption='[[3nk5]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
You may change the PDB parameter (which sets the PDB file loaded into the applet)
== Structural highlights ==
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
<table><tr><td colspan='2'>[[3nk5]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3NK5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3NK5 FirstGlance]. <br>
or leave the SCENE parameter empty for the default display.
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.4&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BOG:B-OCTYLGLUCOSIDE'>BOG</scene></td></tr>
{{STRUCTURE_3nk5| PDB=3nk5 |  SCENE= }}
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3nk5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3nk5 OCA], [https://pdbe.org/3nk5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3nk5 RCSB], [https://www.ebi.ac.uk/pdbsum/3nk5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3nk5 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/AQPZ_ECOLI AQPZ_ECOLI] Channel that permits osmotically driven movement of water in both directions. It is involved in the osmoregulation and in the maintenance of cell turgor during volume expansion in rapidly growing cells. It mediates rapid entry or exit of water in response to abrupt changes in osmolarity.<ref>PMID:10400575</ref> <ref>PMID:10518952</ref> <ref>PMID:11493683</ref>
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/nk/3nk5_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3nk5 ConSurf].
<div style="clear:both"></div>


===Crystal structure of AqpZ mutant F43W===
==See Also==
 
*[[Aquaporin 3D structures|Aquaporin 3D structures]]
 
== References ==
==About this Structure==
<references/>
3NK5 is a 2 chains structure with sequences from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3NK5 OCA].
__TOC__
[[Category: Escherichia coli]]
</StructureSection>
[[Category: Connell, J DO.]]
[[Category: Escherichia coli K-12]]
[[Category: Finer-Moore, J S.]]
[[Category: Large Structures]]
[[Category: Savage, D F.]]
[[Category: Finer-Moore JS]]
[[Category: Stroud, R M.]]
[[Category: O'Connell JD]]
[[Category: Aquaporin]]
[[Category: Savage DF]]
[[Category: Integral membrane protein]]
[[Category: Stroud RM]]
[[Category: Selectivity filter]]
[[Category: Transport protein]]
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Aug 11 23:59:30 2010''

Latest revision as of 13:30, 21 February 2024

Crystal structure of AqpZ mutant F43WCrystal structure of AqpZ mutant F43W

Structural highlights

3nk5 is a 2 chain structure with sequence from Escherichia coli K-12. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 2.4Å
Ligands:
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

AQPZ_ECOLI Channel that permits osmotically driven movement of water in both directions. It is involved in the osmoregulation and in the maintenance of cell turgor during volume expansion in rapidly growing cells. It mediates rapid entry or exit of water in response to abrupt changes in osmolarity.[1] [2] [3]

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

See Also

References

  1. ↑ Delamarche C, Thomas D, Rolland JP, Froger A, Gouranton J, Svelto M, Agre P, Calamita G. Visualization of AqpZ-mediated water permeability in Escherichia coli by cryoelectron microscopy. J Bacteriol. 1999 Jul;181(14):4193-7. PMID:10400575
  2. ↑ Borgnia MJ, Kozono D, Calamita G, Maloney PC, Agre P. Functional reconstitution and characterization of AqpZ, the E. coli water channel protein. J Mol Biol. 1999 Sep 3;291(5):1169-79. PMID:10518952 doi:http://dx.doi.org/S0022-2836(99)93032-2
  3. ↑ Pohl P, Saparov SM, Borgnia MJ, Agre P. Highly selective water channel activity measured by voltage clamp: analysis of planar lipid bilayers reconstituted with purified AqpZ. Proc Natl Acad Sci U S A. 2001 Aug 14;98(17):9624-9. Epub 2001 Aug 7. PMID:11493683 doi:http://dx.doi.org/10.1073/pnas.161299398

3nk5, resolution 2.40Å

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