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==Saccharomyces cerevisiae Cet1-Ceg1 capping apparatus==
==Saccharomyces cerevisiae Cet1-Ceg1 capping apparatus==
<StructureSection load='3kyh' size='340' side='right' caption='[[3kyh]], [[Resolution|resolution]] 3.00&Aring;' scene=''>
<StructureSection load='3kyh' size='340' side='right'caption='[[3kyh]], [[Resolution|resolution]] 3.00&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[3kyh]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_18824 Atcc 18824]. The January 2012 RCSB PDB [http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Messenger RNA Capping''  by David Goodsell is [http://dx.doi.org/10.2210/rcsb_pdb/mom_2012_1 10.2210/rcsb_pdb/mom_2012_1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3KYH OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3KYH FirstGlance]. <br>
<table><tr><td colspan='2'>[[3kyh]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. The January 2012 RCSB PDB [https://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Messenger RNA Capping''  by David Goodsell is [https://dx.doi.org/10.2210/rcsb_pdb/mom_2012_1 10.2210/rcsb_pdb/mom_2012_1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3KYH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3KYH FirstGlance]. <br>
</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">CET1, P1433, YPL228W ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 ATCC 18824]), CEG1, G2853, YGL130W ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 ATCC 18824])</td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3&#8491;</td></tr>
<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Hydrolase Hydrolase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.33 3.1.3.33] </span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3kyh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3kyh OCA], [https://pdbe.org/3kyh PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3kyh RCSB], [https://www.ebi.ac.uk/pdbsum/3kyh PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3kyh ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3kyh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3kyh OCA], [http://pdbe.org/3kyh PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3kyh RCSB], [http://www.ebi.ac.uk/pdbsum/3kyh PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3kyh ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
[[http://www.uniprot.org/uniprot/CET1_YEAST CET1_YEAST]] First step of mRNA capping. Converts the 5'-triphosphate end of a nascent mRNA chain into a diphosphate end.<ref>PMID:12788946</ref> [[http://www.uniprot.org/uniprot/MCE1_YEAST MCE1_YEAST]] Second step of mRNA capping. Transfer of the GMP moiety of GTP to the 5'-end of RNA via an enzyme-GMP covalent reaction intermediate.
[https://www.uniprot.org/uniprot/CET1_YEAST CET1_YEAST] First step of mRNA capping. Converts the 5'-triphosphate end of a nascent mRNA chain into a diphosphate end.<ref>PMID:12788946</ref>  
== Evolutionary Conservation ==
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
Check<jmol>
   <jmolCheckbox>
   <jmolCheckbox>
     <scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ky/3kyh_consurf.spt"</scriptWhenChecked>
     <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ky/3kyh_consurf.spt"</scriptWhenChecked>
     <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
     <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
     <text>to colour the structure by Evolutionary Conservation</text>
     <text>to colour the structure by Evolutionary Conservation</text>
Line 20: Line 19:
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3kyh ConSurf].
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3kyh ConSurf].
<div style="clear:both"></div>
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The 5' guanine-N7 cap is the first cotranscriptional modification of messenger RNA. In Saccharomyces cerevisiae, the first two steps in capping are catalyzed by the RNA triphosphatase Cet1 and RNA guanylyltransferase Ceg1, which form a complex that is directly recruited to phosphorylated RNA polymerase II (RNAP IIo), primarily via contacts between RNAP IIo and Ceg1. A 3.0 A crystal structure of Cet1-Ceg1 revealed a 176 kDa heterotetrameric complex composed of one Cet1 homodimer that associates with two Ceg1 molecules via interactions between the Ceg1 oligonucleotide binding domain and an extended Cet1 WAQKW amino acid motif. The WAQKW motif is followed by a flexible linker that would allow Ceg1 to achieve conformational changes required for capping while maintaining interactions with both Cet1 and RNAP IIo. The impact of mutations as assessed through genetic analysis in S. cerevisiae is consonant with contacts observed in the Cet1-Ceg1 structure.
Structure of the Saccharomyces cerevisiae Cet1-Ceg1 mRNA capping apparatus.,Gu M, Rajashankar KR, Lima CD Structure. 2010 Feb 10;18(2):216-27. PMID:20159466<ref>PMID:20159466</ref>
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 3kyh" style="background-color:#fffaf0;"></div>
== References ==
== References ==
<references/>
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Atcc 18824]]
[[Category: Large Structures]]
[[Category: Hydrolase]]
[[Category: Messenger RNA Capping]]
[[Category: Messenger RNA Capping]]
[[Category: RCSB PDB Molecule of the Month]]
[[Category: RCSB PDB Molecule of the Month]]
[[Category: Lima, C D]]
[[Category: Saccharomyces cerevisiae]]
[[Category: 5' modification]]
[[Category: Lima CD]]
[[Category: Capping]]
[[Category: Complex]]
[[Category: Gtp-binding]]
[[Category: Guanylyltransferase]]
[[Category: Mrna capping]]
[[Category: Mrna processing]]
[[Category: Nucleotide-binding]]
[[Category: Nucleotidyltransferase]]
[[Category: Nucleus]]
[[Category: Phosphoprotein]]
[[Category: Protein binding]]
[[Category: Rna]]
[[Category: Transferase]]
[[Category: Triphosphatase]]

Latest revision as of 13:18, 21 February 2024

Saccharomyces cerevisiae Cet1-Ceg1 capping apparatusSaccharomyces cerevisiae Cet1-Ceg1 capping apparatus

Structural highlights

3kyh is a 4 chain structure with sequence from Saccharomyces cerevisiae. The January 2012 RCSB PDB Molecule of the Month feature on Messenger RNA Capping by David Goodsell is 10.2210/rcsb_pdb/mom_2012_1. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 3Å
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

CET1_YEAST First step of mRNA capping. Converts the 5'-triphosphate end of a nascent mRNA chain into a diphosphate end.[1]

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

References

  1. ↑ Hausmann S, Pei Y, Shuman S. Homodimeric quaternary structure is required for the in vivo function and thermal stability of Saccharomyces cerevisiae and Schizosaccharomyces pombe RNA triphosphatases. J Biol Chem. 2003 Aug 15;278(33):30487-96. Epub 2003 Jun 3. PMID:12788946 doi:http://dx.doi.org/10.1074/jbc.M303060200

3kyh, resolution 3.00Å

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