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==Crystal structure of an uncharacterized protein from Mannheimia succiniciproducens== | ==Crystal structure of an uncharacterized protein from Mannheimia succiniciproducens== | ||
<StructureSection load='3kg4' size='340' side='right' caption='[[3kg4]], [[Resolution|resolution]] 1.95Å' scene=''> | <StructureSection load='3kg4' size='340' side='right'caption='[[3kg4]], [[Resolution|resolution]] 1.95Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[3kg4]] is a 1 chain structure with sequence from [ | <table><tr><td colspan='2'>[[3kg4]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Mannheimia_succiniciproducens_MBEL55E Mannheimia succiniciproducens MBEL55E]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3KG4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3KG4 FirstGlance]. <br> | ||
</td></tr><tr id=' | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.95Å</td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3kg4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3kg4 OCA], [https://pdbe.org/3kg4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3kg4 RCSB], [https://www.ebi.ac.uk/pdbsum/3kg4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3kg4 ProSAT]</span></td></tr> | ||
</table> | </table> | ||
== Function == | == Function == | ||
[ | [https://www.uniprot.org/uniprot/CAS5D_MANSM CAS5D_MANSM] CRISPR (clustered regularly interspaced short palindromic repeat) is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain spacers, sequences complementary to antecedent mobile elements, and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). This protein is a sequence-specific endonuclease that cleaves pre-crRNA at G21 into mature crRNA. Does not cleave pre-crRNA associated with the T.thermophilus strain HB27 Cas5 protein (AC Q746C2) CRISPR locus (PubMed:23006625). The reaction mechanism may proceed by an intramolecular attack of the 2'-hydroxyl group of G21 on the scissile phosphodiester, cutting the precursor 3' to G21 residue yielding 5'-hydroxyl and 2' and/or 3' ends lacking a hydroxyl group (perhaps a 2'/3' cyclic phosphodiester) (Ref.2).<ref>PMID:23006625</ref> <ref>PMID:23006625</ref> | ||
== Evolutionary Conservation == | == Evolutionary Conservation == | ||
[[Image:Consurf_key_small.gif|200px|right]] | [[Image:Consurf_key_small.gif|200px|right]] | ||
Check<jmol> | Check<jmol> | ||
<jmolCheckbox> | <jmolCheckbox> | ||
<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/kg/3kg4_consurf.spt"</scriptWhenChecked> | <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/kg/3kg4_consurf.spt"</scriptWhenChecked> | ||
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> | ||
<text>to colour the structure by Evolutionary Conservation</text> | <text>to colour the structure by Evolutionary Conservation</text> | ||
</jmolCheckbox> | </jmolCheckbox> | ||
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/ | </jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3kg4 ConSurf]. | ||
<div style="clear:both"></div> | <div style="clear:both"></div> | ||
==See Also== | |||
*[[CRISPR subtype I-C|CRISPR subtype I-C]] | |||
*[[CRISPR-Cas|CRISPR-Cas]] | |||
== References == | |||
<references/> | |||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: | [[Category: Large Structures]] | ||
[[Category: Almo | [[Category: Almo SC]] | ||
[[Category: Bain | [[Category: Bain KT]] | ||
[[Category: Bonanno | [[Category: Bonanno JB]] | ||
[[Category: Burley | [[Category: Burley SK]] | ||
[[Category: Dickey | [[Category: Dickey M]] | ||
[[Category: Lau | [[Category: Lau C]] | ||
[[Category: Romero R]] | |||
[[Category: Romero | [[Category: Sauder JM]] | ||
[[Category: Sauder | [[Category: Wasserman S]] | ||
[[Category: Wasserman | |||
Latest revision as of 13:16, 21 February 2024
Crystal structure of an uncharacterized protein from Mannheimia succiniciproducensCrystal structure of an uncharacterized protein from Mannheimia succiniciproducens
Structural highlights
FunctionCAS5D_MANSM CRISPR (clustered regularly interspaced short palindromic repeat) is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain spacers, sequences complementary to antecedent mobile elements, and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). This protein is a sequence-specific endonuclease that cleaves pre-crRNA at G21 into mature crRNA. Does not cleave pre-crRNA associated with the T.thermophilus strain HB27 Cas5 protein (AC Q746C2) CRISPR locus (PubMed:23006625). The reaction mechanism may proceed by an intramolecular attack of the 2'-hydroxyl group of G21 on the scissile phosphodiester, cutting the precursor 3' to G21 residue yielding 5'-hydroxyl and 2' and/or 3' ends lacking a hydroxyl group (perhaps a 2'/3' cyclic phosphodiester) (Ref.2).[1] [2] Evolutionary Conservation Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf. See AlsoReferences
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