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==Core of mammalian 80S pre-ribosome in complex with tRNAs fitted to a 9.8A cryo-EM map: classic PRE state 1==
==Core of mammalian 80S pre-ribosome in complex with tRNAs fitted to a 9.8A cryo-EM map: classic PRE state 1==
<StructureSection load='3j0l' size='340' side='right' caption='[[3j0l]], [[Resolution|resolution]] 9.80&Aring;' scene=''>
<SX load='3j0l' size='340' side='right' viewer='molstar' caption='[[3j0l]], [[Resolution|resolution]] 9.80&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[3j0l]] is a 32 chain structure with sequence from [http://en.wikipedia.org/wiki/Oryctolagus_cuniculus Oryctolagus cuniculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3J0L OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3J0L FirstGlance]. <br>
<table><tr><td colspan='2'>[[3j0l]] is a 13 chain structure with sequence from [https://en.wikipedia.org/wiki/Oryctolagus_cuniculus Oryctolagus cuniculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3J0L OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3J0L FirstGlance]. <br>
</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3j0o|3j0o]], [[3j0p|3j0p]], [[3j0q|3j0q]]</td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 9.8&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3j0l FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3j0l OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3j0l RCSB], [http://www.ebi.ac.uk/pdbsum/3j0l PDBsum]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3j0l FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3j0l OCA], [https://pdbe.org/3j0l PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3j0l RCSB], [https://www.ebi.ac.uk/pdbsum/3j0l PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3j0l ProSAT]</span></td></tr>
</table>
</table>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Although the structural core of the ribosome is conserved in all kingdoms of life, eukaryotic ribosomes are significantly larger and more complex than their bacterial counterparts. The extent to which these differences influence the molecular mechanism of translation remains elusive. Multiparticle cryo-electron microscopy and single-molecule FRET investigations of the mammalian pretranslocation complex reveal spontaneous, large-scale conformational changes, including an intersubunit rotation of the ribosomal subunits. Through structurally related processes, tRNA substrates oscillate between classical and at least two distinct hybrid configurations facilitated by localized changes in their L-shaped fold. Hybrid states are favored within the mammalian complex. However, classical tRNA positions can be restored by tRNA binding to the E site or by the eukaryotic-specific antibiotic and translocation inhibitor cycloheximide. These findings reveal critical distinctions in the structural and energetic features of bacterial and mammalian ribosomes, providing a mechanistic basis for divergent translation regulation strategies and species-specific antibiotic action.
Structure and dynamics of the Mammalian ribosomal pretranslocation complex.,Budkevich T, Giesebrecht J, Altman RB, Munro JB, Mielke T, Nierhaus KH, Blanchard SC, Spahn CM Mol Cell. 2011 Oct 21;44(2):214-24. PMID:22017870<ref>PMID:22017870</ref>
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>


==See Also==
==See Also==
*[[Ribosome 3D structures|Ribosome 3D structures]]
*[[Ribosome 3D structures|Ribosome 3D structures]]
== References ==
*[[Transfer RNA (tRNA)|Transfer RNA (tRNA)]]
<references/>
__TOC__
__TOC__
</StructureSection>
</SX>
[[Category: Large Structures]]
[[Category: Oryctolagus cuniculus]]
[[Category: Oryctolagus cuniculus]]
[[Category: Altman, R]]
[[Category: Altman R]]
[[Category: Blanchard, S]]
[[Category: Blanchard S]]
[[Category: Budkevich, T]]
[[Category: Budkevich T]]
[[Category: Giesebrecht, J]]
[[Category: Giesebrecht J]]
[[Category: Mielke, T]]
[[Category: Mielke T]]
[[Category: Munro, J]]
[[Category: Munro J]]
[[Category: Nierhaus, K]]
[[Category: Nierhaus K]]
[[Category: Spahn, C M]]
[[Category: Spahn CM]]
[[Category: Elongation cycle]]
[[Category: Mammalia]]
[[Category: Ribosome]]
[[Category: Translation]]
[[Category: Trna]]

Latest revision as of 13:09, 21 February 2024

Core of mammalian 80S pre-ribosome in complex with tRNAs fitted to a 9.8A cryo-EM map: classic PRE state 1Core of mammalian 80S pre-ribosome in complex with tRNAs fitted to a 9.8A cryo-EM map: classic PRE state 1

3j0l, resolution 9.80Å

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