3j04: Difference between revisions

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==EM structure of the heavy meromyosin subfragment of Chick smooth muscle Myosin with regulatory light chain in phosphorylated state==
==EM structure of the heavy meromyosin subfragment of Chick smooth muscle Myosin with regulatory light chain in phosphorylated state==
<StructureSection load='3j04' size='340' side='right' caption='[[3j04]], [[Resolution|resolution]] 20.00&Aring;' scene=''>
<SX load='3j04' size='340' side='right' viewer='molstar' caption='[[3j04]], [[Resolution|resolution]] 20.00&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[3j04]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3J04 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3J04 FirstGlance]. <br>
<table><tr><td colspan='2'>[[3j04]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3J04 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3J04 FirstGlance]. <br>
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3j04 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3j04 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3j04 RCSB], [http://www.ebi.ac.uk/pdbsum/3j04 PDBsum]</span></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 20&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3j04 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3j04 OCA], [https://pdbe.org/3j04 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3j04 RCSB], [https://www.ebi.ac.uk/pdbsum/3j04 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3j04 ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
[[http://www.uniprot.org/uniprot/MYH11_CHICK MYH11_CHICK]] Muscle contraction. [[http://www.uniprot.org/uniprot/MYL6_CHICK MYL6_CHICK]] Regulatory light chain of myosin. Does not bind calcium. [[http://www.uniprot.org/uniprot/MLRM_CHICK MLRM_CHICK]] Myosin regulatory subunit that plays an important role in regulation of both smooth muscle and nonmuscle cell contractile activity. Implicated in cytokinesis, receptor capping, and cell locomotion (By similarity).
[https://www.uniprot.org/uniprot/MYL6_CHICK MYL6_CHICK] Regulatory light chain of myosin. Does not bind calcium.
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Smooth muscle myosin and smooth muscle heavy meromyosin (smHMM) are activated by regulatory light chain phosphorylation, but the mechanism remains unclear. Dephosphorylated, inactive smHMM assumes a closed conformation with asymmetric intramolecular head-head interactions between motor domains. The "free head" can bind to actin, but the actin binding interface of the "blocked head" is involved in interactions with the free head. We report here a three-dimensional structure for phosphorylated, active smHMM obtained using electron crystallography of two-dimensional arrays. Head-head interactions of phosphorylated smHMM resemble those found in the dephosphorylated state but occur between different molecules, not within the same molecule. The light chain binding domain structure of phosphorylated smHMM differs markedly from that of the "blocked" head of dephosphorylated smHMM. We hypothesize that regulatory light chain phosphorylation opens the inhibited conformation primarily by its effect on the blocked head. Singly phosphorylated smHMM is not compatible with the closed conformation if the blocked head is phosphorylated. This concept has implications for the extent of myosin activation at low levels of phosphorylation in smooth muscle.
 
Phosphorylated Smooth Muscle Heavy Meromyosin Shows an Open Conformation Linked to Activation.,Baumann BA, Taylor DW, Huang Z, Tama F, Fagnant PM, Trybus KM, Taylor KA J Mol Biol. 2011 Nov 4. PMID:22079364<ref>PMID:22079364</ref>
 
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>


==See Also==
==See Also==
*[[Myosin|Myosin]]
*[[Myosin 3D Structures|Myosin 3D Structures]]
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</SX>
[[Category: Gallus gallus]]
[[Category: Gallus gallus]]
[[Category: Baumann, B A.J]]
[[Category: Large Structures]]
[[Category: Fagnant, P M]]
[[Category: Baumann BAJ]]
[[Category: Huang, Z]]
[[Category: Fagnant PM]]
[[Category: Tama, F]]
[[Category: Huang Z]]
[[Category: Taylor, D]]
[[Category: Tama F]]
[[Category: Taylor, K]]
[[Category: Taylor D]]
[[Category: Trybus, K]]
[[Category: Taylor K]]
[[Category: 2d crystalline array]]
[[Category: Trybus K]]
[[Category: Myosin light chain]]
[[Category: Myosin regulation]]
[[Category: Phosphorylation]]
[[Category: Structural protein]]

Latest revision as of 13:08, 21 February 2024

EM structure of the heavy meromyosin subfragment of Chick smooth muscle Myosin with regulatory light chain in phosphorylated stateEM structure of the heavy meromyosin subfragment of Chick smooth muscle Myosin with regulatory light chain in phosphorylated state

3j04, resolution 20.00Å

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