3e2e: Difference between revisions

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[[Image:3e2e.png|left|200px]]


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==Crystal Structure of an Intermediate Complex of T7 RNAP and 7nt of RNA==
The line below this paragraph, containing "STRUCTURE_3e2e", creates the "Structure Box" on the page.
<StructureSection load='3e2e' size='340' side='right'caption='[[3e2e]], [[Resolution|resolution]] 3.00&Aring;' scene=''>
You may change the PDB parameter (which sets the PDB file loaded into the applet)
== Structural highlights ==
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
<table><tr><td colspan='2'>[[3e2e]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_phage_T7 Escherichia phage T7] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3E2E OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3E2E FirstGlance]. <br>
or leave the SCENE parameter empty for the default display.
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3e2e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3e2e OCA], [https://pdbe.org/3e2e PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3e2e RCSB], [https://www.ebi.ac.uk/pdbsum/3e2e PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3e2e ProSAT]</span></td></tr>
{{STRUCTURE_3e2e| PDB=3e2e |  SCENE= }}
</table>
== Function ==
[https://www.uniprot.org/uniprot/RPOL_BPT7 RPOL_BPT7] DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Responsible for the transcription of the late genes of T7. It is rifampicin-resistant. It recognizes a specific promoter sequence, unwinds the double-stranded RNA to expose the coding strand for templating, initiates transcription preferentially with a purine.
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/e2/3e2e_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3e2e ConSurf].
<div style="clear:both"></div>


===Crystal Structure of an Intermediate Complex of T7 RNAP and 7nt of RNA===
==See Also==
 
*[[RNA polymerase 3D structures|RNA polymerase 3D structures]]
 
__TOC__
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</StructureSection>
The line below this paragraph, {{ABSTRACT_PUBMED_18948533}}, adds the Publication Abstract to the page
[[Category: Escherichia phage T7]]
(as it appears on PubMed at http://www.pubmed.gov), where 18948533 is the PubMed ID number.
[[Category: Large Structures]]
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[[Category: Synthetic construct]]
{{ABSTRACT_PUBMED_18948533}}
[[Category: Bailey S]]
 
[[Category: Durniak KJ]]
==About this Structure==
[[Category: Steitz TA]]
3E2E is a 4 chains structure of sequences from [http://en.wikipedia.org/wiki/Enterobacteria_phage_t7 Enterobacteria phage t7]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3E2E OCA].
 
==Reference==
<ref group="xtra">PMID:18948533</ref><references group="xtra"/>
[[Category: DNA-directed RNA polymerase]]
[[Category: Enterobacteria phage t7]]
[[Category: Bailey, S.]]
[[Category: Durniak, K J.]]
[[Category: Steitz, T A.]]
[[Category: Dna-directed rna polymerase]]
[[Category: Nucleotidyltransferase]]
[[Category: T7 rna polymerase]]
[[Category: Transcription]]
[[Category: Transferase]]
[[Category: Transferase/rna/dna complex]]
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Feb 16 15:42:15 2009''

Latest revision as of 12:44, 21 February 2024

Crystal Structure of an Intermediate Complex of T7 RNAP and 7nt of RNACrystal Structure of an Intermediate Complex of T7 RNAP and 7nt of RNA

Structural highlights

3e2e is a 4 chain structure with sequence from Escherichia phage T7 and Synthetic construct. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 3Å
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

RPOL_BPT7 DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Responsible for the transcription of the late genes of T7. It is rifampicin-resistant. It recognizes a specific promoter sequence, unwinds the double-stranded RNA to expose the coding strand for templating, initiates transcription preferentially with a purine.

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

See Also

3e2e, resolution 3.00Å

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