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| ==Midbody targeting of the ESCRT machinery by a non-canonical coiled-coil in CEP55== | | ==Midbody targeting of the ESCRT machinery by a non-canonical coiled-coil in CEP55== |
| <StructureSection load='3e1r' size='340' side='right' caption='[[3e1r]], [[Resolution|resolution]] 2.00Å' scene=''> | | <StructureSection load='3e1r' size='340' side='right'caption='[[3e1r]], [[Resolution|resolution]] 2.00Å' scene=''> |
| == Structural highlights == | | == Structural highlights == |
| <table><tr><td colspan='2'>[[3e1r]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3E1R OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3E1R FirstGlance]. <br> | | <table><tr><td colspan='2'>[[3e1r]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3E1R OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3E1R FirstGlance]. <br> |
| </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">CEP55, C10orf3, URCC6 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr> | | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2Å</td></tr> |
| <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3e1r FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3e1r OCA], [http://pdbe.org/3e1r PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3e1r RCSB], [http://www.ebi.ac.uk/pdbsum/3e1r PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3e1r ProSAT]</span></td></tr> | | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3e1r FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3e1r OCA], [https://pdbe.org/3e1r PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3e1r RCSB], [https://www.ebi.ac.uk/pdbsum/3e1r PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3e1r ProSAT]</span></td></tr> |
| </table> | | </table> |
| == Function == | | == Function == |
| [[http://www.uniprot.org/uniprot/CEP55_HUMAN CEP55_HUMAN]] Plays a role in mitotic exit and cytokinesis. Not required for microtubule nucleation. Recruits PDCD6IP and TSG101 to midbody during cytokinesis.<ref>PMID:16198290</ref> <ref>PMID:17853893</ref> [[http://www.uniprot.org/uniprot/PDC6I_HUMAN PDC6I_HUMAN]] Class E VPS protein involved in concentration and sorting of cargo proteins of the multivesicular body (MVB) for incorporation into intralumenal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome. Binds to the phospholipid lysobisphosphatidic acid (LBPA) which is abundant in MVBs internal membranes. The MVB pathway appears to require the sequential function of ESCRT-O, -I,-II and -III complexes. The ESCRT machinery also functions in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis and enveloped virus budding (HIV-1 and other lentiviruses). Appears to be an adapter for a subset of ESCRT-III proteins, such as CHMP4, to function at distinct membranes. Required for completion of cytokinesis. Involved in HIV-1 virus budding. Can replace TSG101 it its role of supporting HIV-1 release; this function implies the interaction with CHMP4B. May play a role in the regulation of both apoptosis and cell proliferation.<ref>PMID:14505569</ref> <ref>PMID:14505570</ref> <ref>PMID:14739459</ref> <ref>PMID:17853893</ref> <ref>PMID:17428861</ref> <ref>PMID:17556548</ref> | | [https://www.uniprot.org/uniprot/CEP55_HUMAN CEP55_HUMAN] Plays a role in mitotic exit and cytokinesis. Not required for microtubule nucleation. Recruits PDCD6IP and TSG101 to midbody during cytokinesis.<ref>PMID:16198290</ref> <ref>PMID:17853893</ref> |
| <div style="background-color:#fffaf0;">
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| == Publication Abstract from PubMed ==
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| The ESCRT (endosomal sorting complex required for transport) machinery is required for the scission of membrane necks in processes including the budding of HIV-1 and cytokinesis. An essential step in cytokinesis is recruitment of the ESCRT-I complex and the ESCRT-associated protein ALIX to the midbody (the structure that tethers two daughter cells) by the protein CEP55. Biochemical experiments show that peptides from ALIX and the ESCRT-I subunit TSG101 compete for binding to the ESCRT and ALIX-binding region (EABR) of CEP55. We solved the crystal structure of EABR bound to an ALIX peptide at a resolution of 2.0 angstroms. The structure shows that EABR forms an aberrant dimeric parallel coiled coil. Bulky and charged residues at the interface of the two central heptad repeats create asymmetry and a single binding site for an ALIX or TSG101 peptide. Both ALIX and ESCRT-I are required for cytokinesis, which suggests that multiple CEP55 dimers are required for function.
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| Midbody targeting of the ESCRT machinery by a noncanonical coiled coil in CEP55.,Lee HH, Elia N, Ghirlando R, Lippincott-Schwartz J, Hurley JH Science. 2008 Oct 24;322(5901):576-80. PMID:18948538<ref>PMID:18948538</ref>
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| From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
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| </div>
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| <div class="pdbe-citations 3e1r" style="background-color:#fffaf0;"></div>
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| == References == | | == References == |
| <references/> | | <references/> |
| __TOC__ | | __TOC__ |
| </StructureSection> | | </StructureSection> |
| [[Category: Human]] | | [[Category: Homo sapiens]] |
| [[Category: Elia, N]] | | [[Category: Large Structures]] |
| [[Category: Ghirlando, R]] | | [[Category: Elia N]] |
| [[Category: Hurley, J H]] | | [[Category: Ghirlando R]] |
| [[Category: Lee, H H]] | | [[Category: Hurley JH]] |
| [[Category: Lippincott-Schwartz, J]] | | [[Category: Lee HH]] |
| [[Category: Acetylation]]
| | [[Category: Lippincott-Schwartz J]] |
| [[Category: Alix]]
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| [[Category: Alternative splicing]]
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| [[Category: Apoptosis]]
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| [[Category: Cell cycle]]
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| [[Category: Cell cycle-transport protein complex]]
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| [[Category: Cell division]]
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| [[Category: Cep55]]
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| [[Category: Coiled coil]]
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| [[Category: Cytokinesis]]
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| [[Category: Cytoplasm]]
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| [[Category: Escrt]]
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| [[Category: Host-virus interaction]]
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| [[Category: Mitosis]]
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| [[Category: Phosphoprotein]]
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| [[Category: Polymorphism]]
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| [[Category: Protein transport]]
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| [[Category: Transport]]
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