3dxe: Difference between revisions

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{{Seed}}
[[Image:3dxe.png|left|200px]]


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==Crystal structure of the intracellular domain of human APP (T668A mutant) in complex with Fe65-PTB2==
The line below this paragraph, containing "STRUCTURE_3dxe", creates the "Structure Box" on the page.
<StructureSection load='3dxe' size='340' side='right'caption='[[3dxe]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
You may change the PDB parameter (which sets the PDB file loaded into the applet)  
== Structural highlights ==
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
<table><tr><td colspan='2'>[[3dxe]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3DXE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3DXE FirstGlance]. <br>
or leave the SCENE parameter empty for the default display.
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3dxe FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3dxe OCA], [https://pdbe.org/3dxe PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3dxe RCSB], [https://www.ebi.ac.uk/pdbsum/3dxe PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3dxe ProSAT]</span></td></tr>
{{STRUCTURE_3dxe| PDB=3dxe |  SCENE= }}
</table>
== Function ==
[https://www.uniprot.org/uniprot/APBB1_HUMAN APBB1_HUMAN] Transcription coregulator that can have both coactivator and corepressor functions. Adapter protein that forms a transcriptionally active complex with the gamma-secretase-derived amyloid precursor protein (APP) intracellular domain. Plays a central role in the response to DNA damage by translocating to the nucleus and inducing apoptosis. May act by specifically recognizing and binding histone H2AX phosphorylated on 'Tyr-142' (H2AXY142ph) at double-strand breaks (DSBs), recruiting other pro-apoptosis factors such as MAPK8/JNK1. Required for histone H4 acetylation at double-strand breaks (DSBs). Its ability to specifically bind modified histones and chromatin modifying enzymes such as KAT5/TIP60, probably explains its trancription activation activity. Function in association with TSHZ3, SET and HDAC factors as a transcriptional repressor, that inhibits the expression of CASP4. Associates with chromatin in a region surrounding the CASP4 transcriptional start site(s).<ref>PMID:15031292</ref> <ref>PMID:18468999</ref> <ref>PMID:18922798</ref> <ref>PMID:19234442</ref> <ref>PMID:19343227</ref>
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/dx/3dxe_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3dxe ConSurf].
<div style="clear:both"></div>


===Crystal structure of the intracellular domain of human APP (T668A mutant) in complex with Fe65-PTB2===
==See Also==
 
*[[Amyloid precursor protein 3D structures|Amyloid precursor protein 3D structures]]
 
== References ==
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{{ABSTRACT_PUBMED_18833287}}
 
==About this Structure==
3DXE is a 4 chains structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3DXE OCA].
 
==Reference==
Structure of the intracellular domain of the amyloid precursor protein in complex with Fe65-PTB2., Radzimanowski J, Simon B, Sattler M, Beyreuther K, Sinning I, Wild K, EMBO Rep. 2008 Nov;9(11):1134-40. Epub 2008 Oct 3. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18833287 18833287]
 
Overproduction, purification, crystallization and preliminary X-ray analysis of human Fe65-PTB2 in complex with the amyloid precursor protein intracellular domain., Radzimanowski J, Beyreuther K, Sinning I, Wild K, Acta Crystallogr Sect F Struct Biol Cryst Commun. 2008 May 1;64(Pt, 5):409-12. Epub 2008 Apr 24. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18453713 18453713]
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
[[Category: Radzimanowski, J.]]
[[Category: Large Structures]]
[[Category: Sinning, I.]]
[[Category: Radzimanowski J]]
[[Category: Wild, K.]]
[[Category: Sinning I]]
[[Category: Aicd]]
[[Category: Wild K]]
[[Category: Alternative splicing]]
[[Category: Alzheimer disease]]
[[Category: Alzheimer's disease]]
[[Category: Amyloid]]
[[Category: Apoptosis]]
[[Category: App]]
[[Category: Cell adhesion]]
[[Category: Coated pit]]
[[Category: Copper]]
[[Category: Disease mutation]]
[[Category: Endocytosis]]
[[Category: Fe65]]
[[Category: Glycoprotein]]
[[Category: Heparin-binding]]
[[Category: Iron]]
[[Category: Membrane]]
[[Category: Metal-binding]]
[[Category: Notch signaling pathway]]
[[Category: Phosphoprotein]]
[[Category: Polymorphism]]
[[Category: Protease inhibitor]]
[[Category: Protein binding]]
[[Category: Proteoglycan]]
[[Category: Ptb domain]]
[[Category: Serine protease inhibitor]]
[[Category: Transmembrane]]
[[Category: Zinc]]
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Nov 19 19:50:47 2008''

Latest revision as of 12:43, 21 February 2024

Crystal structure of the intracellular domain of human APP (T668A mutant) in complex with Fe65-PTB2Crystal structure of the intracellular domain of human APP (T668A mutant) in complex with Fe65-PTB2

Structural highlights

3dxe is a 4 chain structure with sequence from Homo sapiens. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 2Å
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

APBB1_HUMAN Transcription coregulator that can have both coactivator and corepressor functions. Adapter protein that forms a transcriptionally active complex with the gamma-secretase-derived amyloid precursor protein (APP) intracellular domain. Plays a central role in the response to DNA damage by translocating to the nucleus and inducing apoptosis. May act by specifically recognizing and binding histone H2AX phosphorylated on 'Tyr-142' (H2AXY142ph) at double-strand breaks (DSBs), recruiting other pro-apoptosis factors such as MAPK8/JNK1. Required for histone H4 acetylation at double-strand breaks (DSBs). Its ability to specifically bind modified histones and chromatin modifying enzymes such as KAT5/TIP60, probably explains its trancription activation activity. Function in association with TSHZ3, SET and HDAC factors as a transcriptional repressor, that inhibits the expression of CASP4. Associates with chromatin in a region surrounding the CASP4 transcriptional start site(s).[1] [2] [3] [4] [5]

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

See Also

References

  1. ↑ Perkinton MS, Standen CL, Lau KF, Kesavapany S, Byers HL, Ward M, McLoughlin DM, Miller CC. The c-Abl tyrosine kinase phosphorylates the Fe65 adaptor protein to stimulate Fe65/amyloid precursor protein nuclear signaling. J Biol Chem. 2004 May 21;279(21):22084-91. Epub 2004 Mar 18. PMID:15031292 doi:10.1074/jbc.M311479200
  2. ↑ Nakaya T, Kawai T, Suzuki T. Regulation of FE65 nuclear translocation and function by amyloid beta-protein precursor in osmotically stressed cells. J Biol Chem. 2008 Jul 4;283(27):19119-31. Epub 2008 May 9. PMID:18468999 doi:M801827200
  3. ↑ Lau KF, Chan WM, Perkinton MS, Tudor EL, Chang RC, Chan HY, McLoughlin DM, Miller CC. Dexras1 interacts with FE65 to regulate FE65-amyloid precursor protein-dependent transcription. J Biol Chem. 2008 Dec 12;283(50):34728-37. Epub 2008 Oct 15. PMID:18922798 doi:M801874200
  4. ↑ Cook PJ, Ju BG, Telese F, Wang X, Glass CK, Rosenfeld MG. Tyrosine dephosphorylation of H2AX modulates apoptosis and survival decisions. Nature. 2009 Apr 2;458(7238):591-6. doi: 10.1038/nature07849. Epub 2009 Feb 22. PMID:19234442 doi:10.1038/nature07849
  5. ↑ Kajiwara Y, Akram A, Katsel P, Haroutunian V, Schmeidler J, Beecham G, Haines JL, Pericak-Vance MA, Buxbaum JD. FE65 binds Teashirt, inhibiting expression of the primate-specific caspase-4. PLoS One. 2009;4(4):e5071. doi: 10.1371/journal.pone.0005071. Epub 2009 Apr 3. PMID:19343227 doi:10.1371/journal.pone.0005071

3dxe, resolution 2.00Å

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