3d31: Difference between revisions

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[[Image:3d31.jpg|left|200px]]


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==ModBC from Methanosarcina acetivorans==
The line below this paragraph, containing "STRUCTURE_3d31", creates the "Structure Box" on the page.
<StructureSection load='3d31' size='340' side='right'caption='[[3d31]], [[Resolution|resolution]] 3.00&Aring;' scene=''>
You may change the PDB parameter (which sets the PDB file loaded into the applet)  
== Structural highlights ==
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
<table><tr><td colspan='2'>[[3d31]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Methanosarcina_acetivorans Methanosarcina acetivorans] and [https://en.wikipedia.org/wiki/Methanosarcina_acetivorans_C2A Methanosarcina acetivorans C2A]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3D31 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3D31 FirstGlance]. <br>
or leave the SCENE parameter empty for the default display.
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=WO4:TUNGSTATE(VI)ION'>WO4</scene></td></tr>
{{STRUCTURE_3d31| PDB=3d31 |  SCENE= }}
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3d31 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3d31 OCA], [https://pdbe.org/3d31 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3d31 RCSB], [https://www.ebi.ac.uk/pdbsum/3d31 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3d31 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/Q8TTZ3_METAC Q8TTZ3_METAC]
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/d3/3d31_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3d31 ConSurf].
<div style="clear:both"></div>


'''ModBC from Methanosarcina acetivorans'''
==See Also==
 
*[[ABC transporter 3D structures|ABC transporter 3D structures]]
 
__TOC__
==Overview==
</StructureSection>
Transport across cellular membranes is an essential process that is catalyzed by diverse membrane transport proteins. The turnover rates of certain transporters are inhibited by their substrates in a process termed trans-inhibition, whose structural basis is poorly understood. Here we present the crystal structure of a molybdate/tungstate ABC transporter (ModBC) from Methanosarcina acetivorans in a trans-inhibited state. The regulatory domains of the nucleotide-binding subunits are in close contact and provide two oxyanion binding pockets at the shared interface. By specifically binding to these pockets, molybdate or tungstate prevent ATPase activity and lock the transporter in an inward-facing conformation, with the catalytic motifs of the nucleotide-binding domains separated. This allosteric effect prevents the transporter from switching between the inward-facing and the outward-facing states, thus interfering with the alternating access and release mechanism.
[[Category: Large Structures]]
 
==About this Structure==
3D31 is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Methanosarcina_acetivorans Methanosarcina acetivorans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3D31 OCA].
 
==Reference==
Structural Basis of Trans-Inhibition in a Molybdate/tungstate ABC Transporter., Gerber S, Comellas-Bigler M, Goetz BA, Locher KP, Science. 2008 May 29;. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18511655 18511655]
[[Category: Methanosarcina acetivorans]]
[[Category: Methanosarcina acetivorans]]
[[Category: Protein complex]]
[[Category: Methanosarcina acetivorans C2A]]
[[Category: Comellas-Bigler, M.]]
[[Category: Comellas-Bigler M]]
[[Category: Gerber, S.]]
[[Category: Gerber S]]
[[Category: Locher, K P.]]
[[Category: Locher KP]]
[[Category: Atp-binding]]
[[Category: Membrane]]
[[Category: Nucleotide-binding]]
[[Category: Transmembrane]]
[[Category: Transport]]
[[Category: Transport protein]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jun 11 10:51:13 2008''

Latest revision as of 12:39, 21 February 2024

ModBC from Methanosarcina acetivoransModBC from Methanosarcina acetivorans

Structural highlights

3d31 is a 4 chain structure with sequence from Methanosarcina acetivorans and Methanosarcina acetivorans C2A. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 3Å
Ligands:
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

Q8TTZ3_METAC

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

See Also

3d31, resolution 3.00Å

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