2sli: Difference between revisions

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[[Image:2sli.gif|left|200px]]


{{Structure
==LEECH INTRAMOLECULAR TRANS-SIALIDASE COMPLEXED WITH 2,7-ANHYDRO-NEU5AC, THE REACTION PRODUCT==
|PDB= 2sli |SIZE=350|CAPTION= <scene name='initialview01'>2sli</scene>, resolution 1.8&Aring;
<StructureSection load='2sli' size='340' side='right'caption='[[2sli]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
|SITE=  
== Structural highlights ==
|LIGAND= <scene name='pdbligand=SKD:2-ACETYLAMINO-7-(1,2-DIHYDROXY-ETHYL)-3-HYDROXY-6,8-DIOXA-BICYCLO[3.2.1]OCTANE-5-CARBOXYLIC ACID'>SKD</scene>
<table><tr><td colspan='2'>[[2sli]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Macrobdella_decora Macrobdella decora]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2SLI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2SLI FirstGlance]. <br>
|ACTIVITY= [http://en.wikipedia.org/wiki/Exo-alpha-sialidase Exo-alpha-sialidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.18 3.2.1.18]  
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8&#8491;</td></tr>
|GENE= T7 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=6405 Macrobdella decora])
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SKD:2-ACETYLAMINO-7-(1,2-DIHYDROXY-ETHYL)-3-HYDROXY-6,8-DIOXA-BICYCLO[3.2.1]OCTANE-5-CARBOXYLIC+ACID'>SKD</scene></td></tr>
}}
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2sli FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2sli OCA], [https://pdbe.org/2sli PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2sli RCSB], [https://www.ebi.ac.uk/pdbsum/2sli PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2sli ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/NANL_MACDE NANL_MACDE]  
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/sl/2sli_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2sli ConSurf].
<div style="clear:both"></div>


'''LEECH INTRAMOLECULAR TRANS-SIALIDASE COMPLEXED WITH 2,7-ANHYDRO-NEU5AC, THE REACTION PRODUCT'''
==See Also==
 
*[[Neuraminidase 3D structures|Neuraminidase 3D structures]]
 
__TOC__
==Overview==
</StructureSection>
Intramolecular trans-sialidase from leech (Macrobdella decora) is the first member of the sialidase superfamily found to exhibit strict specificity towards the cleavage of terminal Neu5Acalpha2--&gt;3Gal linkage in sialoglycoconjugates. Its release of 2,7-anhydro-Neu5Ac instead of Neu5Ac indicates that it catalyzes an intramolecular trans-sialosyl reaction. Crystal structures of its complexes with an inactive substrate analogue 2-propenyl-Neu5Ac, and with the product 2,7-anhydro-Neu5Ac, have been determined to 1.8 A resolution. The boat conformation of the pyranose observed in the complexes supports the proposed enzymatic mechanism that O7 of an axial 6-glycerol group attacks the positively charged C2 of the intermediate. A generalized mechanism is proposed for the sialidase superfamily.
[[Category: Large Structures]]
 
==About this Structure==
2SLI is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Macrobdella_decora Macrobdella decora]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2SLI OCA].
 
==Reference==
The 1.8 A structures of leech intramolecular trans-sialidase complexes: evidence of its enzymatic mechanism., Luo Y, Li SC, Li YT, Luo M, J Mol Biol. 1999 Jan 8;285(1):323-32. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9878409 9878409]
[[Category: Exo-alpha-sialidase]]
[[Category: Macrobdella decora]]
[[Category: Macrobdella decora]]
[[Category: Single protein]]
[[Category: Li SC]]
[[Category: Li, S C.]]
[[Category: Li YT]]
[[Category: Li, Y T.]]
[[Category: Luo M]]
[[Category: Luo, M.]]
[[Category: Luo Y]]
[[Category: Luo, Y.]]
[[Category: SKD]]
[[Category: hydrolase]]
[[Category: intramolecular trans-sialidase]]
[[Category: neuraminidase]]
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 18:38:30 2008''

Latest revision as of 12:23, 21 February 2024

LEECH INTRAMOLECULAR TRANS-SIALIDASE COMPLEXED WITH 2,7-ANHYDRO-NEU5AC, THE REACTION PRODUCTLEECH INTRAMOLECULAR TRANS-SIALIDASE COMPLEXED WITH 2,7-ANHYDRO-NEU5AC, THE REACTION PRODUCT

Structural highlights

2sli is a 1 chain structure with sequence from Macrobdella decora. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 1.8Å
Ligands:
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

NANL_MACDE

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

See Also

2sli, resolution 1.80Å

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