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| [[Image:2rc6.jpg|left|200px]]
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| {{Structure
| | ==Refined structure of FNR from Leptospira interrogans bound to NADP+== |
| |PDB= 2rc6 |SIZE=350|CAPTION= <scene name='initialview01'>2rc6</scene>, resolution 2.700Å
| | <StructureSection load='2rc6' size='340' side='right'caption='[[2rc6]], [[Resolution|resolution]] 2.70Å' scene=''> |
| |SITE= <scene name='pdbsite=AC1:Zn+Binding+Site+For+Residue+A+315'>AC1</scene>, <scene name='pdbsite=AC2:So4+Binding+Site+For+Residue+A+316'>AC2</scene>, <scene name='pdbsite=AC3:So4+Binding+Site+For+Residue+B+316'>AC3</scene>, <scene name='pdbsite=AC4:So4+Binding+Site+For+Residue+C+315'>AC4</scene>, <scene name='pdbsite=AC5:So4+Binding+Site+For+Residue+C+316'>AC5</scene>, <scene name='pdbsite=AC6:So4+Binding+Site+For+Residue+D+315'>AC6</scene>, <scene name='pdbsite=AC7:So4+Binding+Site+For+Residue+D+316'>AC7</scene>, <scene name='pdbsite=AC8:Fad+Binding+Site+For+Residue+A+415'>AC8</scene>, <scene name='pdbsite=AC9:Nap+Binding+Site+For+Residue+A+416'>AC9</scene>, <scene name='pdbsite=BC1:Fad+Binding+Site+For+Residue+B+415'>BC1</scene>, <scene name='pdbsite=BC2:Nap+Binding+Site+For+Residue+B+416'>BC2</scene>, <scene name='pdbsite=BC3:Fad+Binding+Site+For+Residue+C+415'>BC3</scene>, <scene name='pdbsite=BC4:Nap+Binding+Site+For+Residue+C+416'>BC4</scene>, <scene name='pdbsite=BC5:Fad+Binding+Site+For+Residue+D+415'>BC5</scene> and <scene name='pdbsite=BC6:Nap+Binding+Site+For+Residue+D+416'>BC6</scene>
| | == Structural highlights == |
| |LIGAND= <scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=NAP:NADP+NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NAP</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene>
| | <table><tr><td colspan='2'>[[2rc6]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Leptospira_interrogans Leptospira interrogans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2RC6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2RC6 FirstGlance]. <br> |
| |ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Ferredoxin--NADP(+)_reductase Ferredoxin--NADP(+) reductase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.18.1.2 1.18.1.2] </span>
| | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.7Å</td></tr> |
| |GENE=
| | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=NAP:NADP+NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NAP</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> |
| |DOMAIN=<span class='plainlinks'>[http://www.ncbi.nlm.nih.gov/Structure/cdd/cddsrv.cgi?uid=pfam00175 NAD_binding_1], [http://www.ncbi.nlm.nih.gov/Structure/cdd/cddsrv.cgi?uid=COG0543 UbiB]</span>
| | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2rc6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2rc6 OCA], [https://pdbe.org/2rc6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2rc6 RCSB], [https://www.ebi.ac.uk/pdbsum/2rc6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2rc6 ProSAT]</span></td></tr> |
| |RELATEDENTRY=[[2rc5|2RC5]]
| | </table> |
| |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2rc6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2rc6 OCA], [http://www.ebi.ac.uk/pdbsum/2rc6 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2rc6 RCSB]</span>
| | == Function == |
| }}
| | [https://www.uniprot.org/uniprot/Q8EY89_LEPIN Q8EY89_LEPIN] |
| | | == Evolutionary Conservation == |
| '''Refined structure of FNR from Leptospira interrogans bound to NADP+'''
| | [[Image:Consurf_key_small.gif|200px|right]] |
| | | Check<jmol> |
| | | <jmolCheckbox> |
| ==Overview== | | <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/rc/2rc6_consurf.spt"</scriptWhenChecked> |
| BACKGROUND: Ferredoxin-NADP(H) reductases (FNRs) are flavoenzymes that catalyze the electron transfer between NADP(H) and the proteins ferredoxin or flavodoxin. A number of structural features distinguish plant and bacterial FNRs, one of which is the mode of the cofactor FAD binding. Leptospira interrogans is a spirochaete parasitic bacterium capable of infecting humans and mammals in general. Leptospira interrogans FNR (LepFNR) displays low sequence identity with plant (34% with Zea mays) and bacterial (31% with Escherichia coli) FNRs. However, LepFNR contains all consensus sequences that define the plastidic class FNRs. RESULTS: The crystal structures of the FAD-containing LepFNR and the complex of the enzyme with NADP+, were solved and compared to known FNRs. The comparison reveals significant structural similarities of the enzyme with the plastidic type FNRs and differences with the bacterial enzymes. Our small angle X-ray scattering experiments show that LepFNR is a monomeric enzyme. Moreover, our biochemical data demonstrate that the LepFNR has an enzymatic activity similar to those reported for the plastidic enzymes and that is significantly different from bacterial flavoenzymes, which display lower turnover rates. CONCLUSION: LepFNR is the first plastidic type FNR found in bacteria and, despite of its low sequence similarity with plastidic FNRs still displays high catalytic turnover rates. The typical structural and biochemical characteristics of plant FNRs unveiled for LepFNR support a notion of a putative lateral gene transfer which presumably offers Leptospira interrogans evolutionary advantages. The wealth of structural information about LepFNR provides a molecular basis for advanced drugs developments against leptospirosis.
| | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> |
| | | <text>to colour the structure by Evolutionary Conservation</text> |
| ==About this Structure== | | </jmolCheckbox> |
| 2RC6 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Leptospira_interrogans Leptospira interrogans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2RC6 OCA].
| | </jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2rc6 ConSurf]. |
| | | <div style="clear:both"></div> |
| ==Reference==
| | __TOC__ |
| Crystal structures of Leptospira interrogans FAD-containing ferredoxin-NADP+ reductase and its complex with NADP+., Nascimento AS, Catalano-Dupuy DL, Bernardes A, Neto Mde O, Santos MA, Ceccarelli EA, Polikarpov I, BMC Struct Biol. 2007 Oct 24;7:69. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17958910 17958910]
| | </StructureSection> |
| [[Category: Ferredoxin--NADP(+) reductase]] | | [[Category: Large Structures]] |
| [[Category: Leptospira interrogans]] | | [[Category: Leptospira interrogans]] |
| [[Category: Single protein]]
| | [[Category: Catalano-Dupuy DL]] |
| [[Category: Catalano-Dupuy, D L.]] | | [[Category: Ceccarelli EA]] |
| [[Category: Ceccarelli, E A.]] | | [[Category: Nascimento AS]] |
| [[Category: Nascimento, A S.]] | | [[Category: Polikarpov I]] |
| [[Category: Polikarpov, I.]] | |
| [[Category: fnr]]
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| [[Category: oxidoreductase]]
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| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 04:58:47 2008''
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