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| [[Image:2qvj.jpg|left|200px]]
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| {{Structure
| | ==Crystal structure of a vesicular stomatitis virus nucleocapsid protein Ser290Trp mutant== |
| |PDB= 2qvj |SIZE=350|CAPTION= <scene name='initialview01'>2qvj</scene>, resolution 2.800Å
| | <StructureSection load='2qvj' size='340' side='right'caption='[[2qvj]], [[Resolution|resolution]] 2.80Å' scene=''> |
| |SITE=
| | == Structural highlights == |
| |LIGAND=
| | <table><tr><td colspan='2'>[[2qvj]] is a 5 chain structure with sequence from [https://en.wikipedia.org/wiki/Vesicular_stomatitis_Indiana_virus Vesicular stomatitis Indiana virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2QVJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2QVJ FirstGlance]. <br> |
| |ACTIVITY=
| | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.8Å</td></tr> |
| |GENE= N ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=11277 Vesicular stomatitis Indiana virus])
| | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2qvj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2qvj OCA], [https://pdbe.org/2qvj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2qvj RCSB], [https://www.ebi.ac.uk/pdbsum/2qvj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2qvj ProSAT]</span></td></tr> |
| }}
| | </table> |
| | == Function == |
| | [https://www.uniprot.org/uniprot/A6H4P1_9RHAB A6H4P1_9RHAB] |
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| '''Crystal structure of a vesicular stomatitis virus nucleocapsid protein Ser290Trp mutant'''
| | ==See Also== |
| | | *[[Nucleoprotein 3D structures|Nucleoprotein 3D structures]] |
| | | __TOC__ |
| ==Overview== | | </StructureSection> |
| The crystal structure of the vesicular stomatitis virus nucleoprotein (N) in complex with RNA reveals extensive and specific intermolecular interactions among the N molecules in the 10-member oligomer. What roles these interactions play in encapsidating RNA was studied by mutagenesis of the N protein. Three N mutants intended for disruption of the intermolecular interactions were designed and coexpressed with the phosphoprotein (P) in an Escherichia coli system previously described (T. J. Green et al., J. Virol. 74:9515-9524, 2000). Mutants N (Delta1-22), N (Delta347-352), and N (320-324, (Ala)(5)) lost RNA encapsidation and oligomerization but still bound with P. Another mutant, N (Ser290-->Trp), was able to form a stable ring-like N oligomer and bind with the P protein but was no longer able to encapsidate RNA. The crystal structure of N (Ser290-->Trp) at 2.8 A resolution showed that this mutant can maintain all the same intermolecular interactions as the wild-type N except for a slight unwinding of the N-terminal lobe. These results suggest that the intermolecular contacts among the N molecules are required for encapsidation of the viral RNA.
| | [[Category: Large Structures]] |
| | | [[Category: Vesicular stomatitis Indiana virus]] |
| ==About this Structure==
| | [[Category: Green TJ]] |
| 2QVJ is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Vesicular_stomatitis_indiana_virus Vesicular stomatitis indiana virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2QVJ OCA].
| | [[Category: Luo M]] |
| | | [[Category: Qiu S]] |
| ==Reference==
| | [[Category: Tsao J]] |
| Role of intermolecular interactions of vesicular stomatitis virus nucleoprotein in RNA encapsidation., Zhang X, Green TJ, Tsao J, Qiu S, Luo M, J Virol. 2008 Jan;82(2):674-82. Epub 2007 Nov 14. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18003727 18003727]
| | [[Category: Zhang X]] |
| [[Category: Single protein]] | |
| [[Category: Vesicular stomatitis indiana virus]] | |
| [[Category: Green, T J.]] | |
| [[Category: Luo, M.]] | |
| [[Category: Qiu, S.]] | |
| [[Category: Tsao, J.]] | |
| [[Category: Zhang, X.]] | |
| [[Category: cytoplasm]]
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| [[Category: nucleocapsid]]
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| [[Category: ribonucleoprotein]]
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| [[Category: rna binding protein]]
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| [[Category: rna-binding]]
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| [[Category: viral nucleoprotein]]
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| [[Category: virion]]
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| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 18:29:59 2008''
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