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==Crystal structure of a vesicular stomatitis virus nucleocapsid protein Ser290Trp mutant==
==Crystal structure of a vesicular stomatitis virus nucleocapsid protein Ser290Trp mutant==
<StructureSection load='2qvj' size='340' side='right' caption='[[2qvj]], [[Resolution|resolution]] 2.80&Aring;' scene=''>
<StructureSection load='2qvj' size='340' side='right'caption='[[2qvj]], [[Resolution|resolution]] 2.80&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[2qvj]] is a 5 chain structure with sequence from [http://en.wikipedia.org/wiki/Vesicular_stomatitis_indiana_virus Vesicular stomatitis indiana virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2QVJ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2QVJ FirstGlance]. <br>
<table><tr><td colspan='2'>[[2qvj]] is a 5 chain structure with sequence from [https://en.wikipedia.org/wiki/Vesicular_stomatitis_Indiana_virus Vesicular stomatitis Indiana virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2QVJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2QVJ FirstGlance]. <br>
</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">N ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=11277 Vesicular stomatitis Indiana virus])</td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.8&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2qvj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2qvj OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2qvj RCSB], [http://www.ebi.ac.uk/pdbsum/2qvj PDBsum]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2qvj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2qvj OCA], [https://pdbe.org/2qvj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2qvj RCSB], [https://www.ebi.ac.uk/pdbsum/2qvj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2qvj ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
[[http://www.uniprot.org/uniprot/NCAP_VSIVA NCAP_VSIVA]] Encapsidates the genome in a ratio of one N per nine ribonucleotides, protecting it from nucleases. The encapsidated genomic RNA is termed the NC and serves as template for transcription and replication. Replication is dependent on intracellular concentration of newly synthesized N, termed N(0), which corresponds to the protein not associated with RNA. In contrast, when associated with RNA, it is termed N. During replication, encapsidation by N(0) is coupled to RNA synthesis and all replicative products are resistant to nucleases (By similarity).
[https://www.uniprot.org/uniprot/A6H4P1_9RHAB A6H4P1_9RHAB]  
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The crystal structure of the vesicular stomatitis virus nucleoprotein (N) in complex with RNA reveals extensive and specific intermolecular interactions among the N molecules in the 10-member oligomer. What roles these interactions play in encapsidating RNA was studied by mutagenesis of the N protein. Three N mutants intended for disruption of the intermolecular interactions were designed and coexpressed with the phosphoprotein (P) in an Escherichia coli system previously described (T. J. Green et al., J. Virol. 74:9515-9524, 2000). Mutants N (Delta1-22), N (Delta347-352), and N (320-324, (Ala)(5)) lost RNA encapsidation and oligomerization but still bound with P. Another mutant, N (Ser290--&gt;Trp), was able to form a stable ring-like N oligomer and bind with the P protein but was no longer able to encapsidate RNA. The crystal structure of N (Ser290--&gt;Trp) at 2.8 A resolution showed that this mutant can maintain all the same intermolecular interactions as the wild-type N except for a slight unwinding of the N-terminal lobe. These results suggest that the intermolecular contacts among the N molecules are required for encapsidation of the viral RNA.
 
Role of intermolecular interactions of vesicular stomatitis virus nucleoprotein in RNA encapsidation.,Zhang X, Green TJ, Tsao J, Qiu S, Luo M J Virol. 2008 Jan;82(2):674-82. Epub 2007 Nov 14. PMID:18003727<ref>PMID:18003727</ref>
 
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>


==See Also==
==See Also==
*[[Nucleoprotein|Nucleoprotein]]
*[[Nucleoprotein 3D structures|Nucleoprotein 3D structures]]
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Vesicular stomatitis indiana virus]]
[[Category: Large Structures]]
[[Category: Green, T J]]
[[Category: Vesicular stomatitis Indiana virus]]
[[Category: Luo, M]]
[[Category: Green TJ]]
[[Category: Qiu, S]]
[[Category: Luo M]]
[[Category: Tsao, J]]
[[Category: Qiu S]]
[[Category: Zhang, X]]
[[Category: Tsao J]]
[[Category: Nucleocapsid]]
[[Category: Zhang X]]
[[Category: Ribonucleoprotein]]
[[Category: Rna binding protein]]
[[Category: Rna-binding]]
[[Category: Viral nucleoprotein]]
[[Category: Virion]]

Latest revision as of 12:18, 21 February 2024

Crystal structure of a vesicular stomatitis virus nucleocapsid protein Ser290Trp mutantCrystal structure of a vesicular stomatitis virus nucleocapsid protein Ser290Trp mutant

Structural highlights

2qvj is a 5 chain structure with sequence from Vesicular stomatitis Indiana virus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 2.8Å
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

A6H4P1_9RHAB

See Also

2qvj, resolution 2.80Å

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