2qus: Difference between revisions

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==Hammerhead Ribozyme G12A mutant pre-cleavage==
==Hammerhead Ribozyme G12A mutant pre-cleavage==
<StructureSection load='2qus' size='340' side='right' caption='[[2qus]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
<StructureSection load='2qus' size='340' side='right'caption='[[2qus]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[2qus]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2QUS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2QUS FirstGlance]. <br>
<table><tr><td colspan='2'>[[2qus]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Tobacco_ringspot_virus_satellite_RNA Tobacco ringspot virus satellite RNA]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2QUS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2QUS FirstGlance]. <br>
</td></tr><tr><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene><br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.4&#8491;</td></tr>
<tr><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=GTP:GUANOSINE-5-TRIPHOSPHATE'>GTP</scene></td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GTP:GUANOSINE-5-TRIPHOSPHATE'>GTP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
<tr><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2goz|2goz]], [[2quw|2quw]]</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2qus FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2qus OCA], [https://pdbe.org/2qus PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2qus RCSB], [https://www.ebi.ac.uk/pdbsum/2qus PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2qus ProSAT]</span></td></tr>
<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2qus FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2qus OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2qus RCSB], [http://www.ebi.ac.uk/pdbsum/2qus PDBsum]</span></td></tr>
</table>
<table>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
We have obtained precatalytic (enzyme-substrate complex) and postcatalytic (enzyme-product complex) crystal structures of an active full-length hammerhead RNA that cleaves in the crystal. Using the natural satellite tobacco ringspot virus hammerhead RNA sequence, the self-cleavage reaction was modulated by substituting the general base of the ribozyme, G12, with A12, a purine variant with a much lower pKa that does not significantly perturb the ribozyme's atomic structure. The active, but slowly cleaving, ribozyme thus permitted isolation of enzyme-substrate and enzyme-product complexes without modifying the nucleophile or leaving group of the cleavage reaction, nor any other aspect of the substrate. The predissociation enzyme-product complex structure reveals RNA and metal ion interactions potentially relevant to transition-state stabilization that are absent in precatalytic structures.
 
Capturing hammerhead ribozyme structures in action by modulating general base catalysis.,Chi YI, Martick M, Lares M, Kim R, Scott WG, Kim SH PLoS Biol. 2008 Sep 30;6(9):e234. PMID:18834200<ref>PMID:18834200</ref>
 
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>


==See Also==
==See Also==
*[[Ribozyme|Ribozyme]]
*[[Ribozyme 3D structures|Ribozyme 3D structures]]
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Chi, Y I.]]
[[Category: Large Structures]]
[[Category: Kim, S H.]]
[[Category: Tobacco ringspot virus satellite RNA]]
[[Category: Scott, W G.]]
[[Category: Chi YI]]
[[Category: Catalytic rna]]
[[Category: Kim SH]]
[[Category: Hammerhead ribozyme]]
[[Category: Scott WG]]
[[Category: Hammerhead rna]]
[[Category: Ribozyme]]
[[Category: Rna]]
[[Category: Satellite virus rna]]

Latest revision as of 12:18, 21 February 2024

Hammerhead Ribozyme G12A mutant pre-cleavageHammerhead Ribozyme G12A mutant pre-cleavage

Structural highlights

2qus is a 2 chain structure with sequence from Tobacco ringspot virus satellite RNA. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 2.4Å
Ligands:,
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

See Also

2qus, resolution 2.40Å

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