2q6t: Difference between revisions

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New page: left|200px<br /><applet load="2q6t" size="350" color="white" frame="true" align="right" spinBox="true" caption="2q6t, resolution 2.90Å" /> '''Crystal structure of...
 
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[[Image:2q6t.gif|left|200px]]<br /><applet load="2q6t" size="350" color="white" frame="true" align="right" spinBox="true"
caption="2q6t, resolution 2.90&Aring;" />
'''Crystal structure of the Thermus aquaticus DnaB monomer'''<br />


==Overview==
==Crystal structure of the Thermus aquaticus DnaB monomer==
The ring-shaped hexameric DnaB helicase unwinds duplex DNA at the, replication fork of eubacteria. We have solved the crystal structure of, the full-length Thermus aquaticus DnaB monomer, or possibly dimer, at 2.9, A resolution. DnaB is a highly flexible two domain protein. The C-terminal, domain exhibits a RecA-like core fold and contains all the conserved, sequence motifs that are characteristic of the DnaB helicase family. The, N-terminal domain contains an additional helical hairpin that makes it, larger than previously appreciated. Several DnaB mutations that modulate, its interaction with primase are found in this hairpin. The similarity in, the fold of the DnaB N-terminal domain with that of the C-terminal, helicase-binding domain (HBD) of the DnaG primase also includes this, hairpin. Comparison of hexameric homology models of DnaB with the, structure of the papillomavirus E1 helicase suggests the two helicases may, function through different mechanisms despite their sharing a common, ancestor.
<StructureSection load='2q6t' size='340' side='right'caption='[[2q6t]], [[Resolution|resolution]] 2.90&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[2q6t]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermus_aquaticus Thermus aquaticus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2Q6T OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2Q6T FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.9&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2q6t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2q6t OCA], [https://pdbe.org/2q6t PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2q6t RCSB], [https://www.ebi.ac.uk/pdbsum/2q6t PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2q6t ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/Q9Z693_THEAQ Q9Z693_THEAQ]
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/q6/2q6t_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2q6t ConSurf].
<div style="clear:both"></div>


==About this Structure==
==See Also==
2Q6T is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Thermus_aquaticus Thermus aquaticus] with <scene name='pdbligand=SO4:'>SO4</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2Q6T OCA].
*[[Helicase 3D structures|Helicase 3D structures]]
 
__TOC__
==Reference==
</StructureSection>
The crystal structure of the Thermus aquaticus DnaB helicase monomer., Bailey S, Eliason WK, Steitz TA, Nucleic Acids Res. 2007 Jul 1;. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17606462 17606462]
[[Category: Large Structures]]
[[Category: Single protein]]
[[Category: Thermus aquaticus]]
[[Category: Thermus aquaticus]]
[[Category: Bailey, S.]]
[[Category: Bailey S]]
[[Category: Eliason, W.K.]]
[[Category: Eliason WK]]
[[Category: Steitz, T.A.]]
[[Category: Steitz TA]]
[[Category: SO4]]
[[Category: dnab]]
[[Category: helicase]]
[[Category: replication]]
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jan 23 13:57:37 2008''

Latest revision as of 12:14, 21 February 2024

Crystal structure of the Thermus aquaticus DnaB monomerCrystal structure of the Thermus aquaticus DnaB monomer

Structural highlights

2q6t is a 4 chain structure with sequence from Thermus aquaticus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 2.9Å
Ligands:
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

Q9Z693_THEAQ

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

See Also

2q6t, resolution 2.90Å

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