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[[Image:2q5e.jpg|left|200px]]


{{Structure
==Crystal structure of human carboxy-terminal domain RNA polymerase II polypeptide A small phosphatase 2==
|PDB= 2q5e |SIZE=350|CAPTION= <scene name='initialview01'>2q5e</scene>, resolution 2.510&Aring;
<StructureSection load='2q5e' size='340' side='right'caption='[[2q5e]], [[Resolution|resolution]] 2.51&Aring;' scene=''>
|SITE= <scene name='pdbsite=AC1:Mg+Binding+Site+For+Residue+A+1'>AC1</scene>, <scene name='pdbsite=AC2:Mg+Binding+Site+For+Residue+B+1'>AC2</scene>, <scene name='pdbsite=AC3:Mg+Binding+Site+For+Residue+C+1'>AC3</scene>, <scene name='pdbsite=AC4:Mg+Binding+Site+For+Residue+D+1'>AC4</scene>, <scene name='pdbsite=AC5:Mg+Binding+Site+For+Residue+E+1'>AC5</scene>, <scene name='pdbsite=AC6:Mg+Binding+Site+For+Residue+F+1'>AC6</scene>, <scene name='pdbsite=AC7:Mg+Binding+Site+For+Residue+G+1'>AC7</scene> and <scene name='pdbsite=AC8:Mg+Binding+Site+For+Residue+H+1'>AC8</scene>
== Structural highlights ==
|LIGAND= <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>
<table><tr><td colspan='2'>[[2q5e]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2Q5E OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2Q5E FirstGlance]. <br>
|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Phosphoprotein_phosphatase Phosphoprotein phosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.16 3.1.3.16] </span>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.51&#8491;</td></tr>
|GENE= CTDSP2, NIF2, OS4 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
|DOMAIN=<span class='plainlinks'>[http://www.ncbi.nlm.nih.gov/Structure/cdd/cddsrv.cgi?uid=pfam03031 NIF]</span>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2q5e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2q5e OCA], [https://pdbe.org/2q5e PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2q5e RCSB], [https://www.ebi.ac.uk/pdbsum/2q5e PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2q5e ProSAT], [https://www.topsan.org/Proteins/NYSGXRC/2q5e TOPSAN]</span></td></tr>
|RELATEDENTRY=
</table>
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2q5e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2q5e OCA], [http://www.ebi.ac.uk/pdbsum/2q5e PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2q5e RCSB]</span>
== Function ==
}}
[https://www.uniprot.org/uniprot/CTDS2_HUMAN CTDS2_HUMAN] Preferentially catalyzes the dephosphorylation of 'Ser-5' within the tandem 7 residues repeats in the C-terminal domain (CTD) of the largest RNA polymerase II subunit POLR2A. Negatively regulates RNA polymerase II transcription, possibly by controlling the transition from initiation/capping to processive transcript elongation. Recruited by REST to neuronal genes that contain RE-1 elements, leading to neuronal gene silencing in non-neuronal cells. May contribute to the development of sarcomas.<ref>PMID:12721286</ref> <ref>PMID:15681389</ref>
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/q5/2q5e_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2q5e ConSurf].
<div style="clear:both"></div>


'''Crystal structure of human carboxy-terminal domain RNA polymerase II polypeptide A small phosphatase 2'''
==See Also==
 
*[[RNA polymerase 3D structures|RNA polymerase 3D structures]]
 
== References ==
==Overview==
<references/>
The New York SGX Research Center for Structural Genomics (NYSGXRC) of the NIGMS Protein Structure Initiative (PSI) has applied its high-throughput X-ray crystallographic structure determination platform to systematic studies of all human protein phosphatases and protein phosphatases from biomedically-relevant pathogens. To date, the NYSGXRC has determined structures of 21 distinct protein phosphatases: 14 from human, 2 from mouse, 2 from the pathogen Toxoplasma gondii, 1 from Trypanosoma brucei, the parasite responsible for African sleeping sickness, and 2 from the principal mosquito vector of malaria in Africa, Anopheles gambiae. These structures provide insights into both normal and pathophysiologic processes, including transcriptional regulation, regulation of major signaling pathways, neural development, and type 1 diabetes. In conjunction with the contributions of other international structural genomics consortia, these efforts promise to provide an unprecedented database and materials repository for structure-guided experimental and computational discovery of inhibitors for all classes of protein phosphatases.
__TOC__
 
</StructureSection>
==About this Structure==
2Q5E is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2Q5E OCA].
 
==Reference==
Structural genomics of protein phosphatases., Almo SC, Bonanno JB, Sauder JM, Emtage S, Dilorenzo TP, Malashkevich V, Wasserman SR, Swaminathan S, Eswaramoorthy S, Agarwal R, Kumaran D, Madegowda M, Ragumani S, Patskovsky Y, Alvarado J, Ramagopal UA, Faber-Barata J, Chance MR, Sali A, Fiser A, Zhang ZY, Lawrence DS, Burley SK, J Struct Funct Genomics. 2007 Sep;8(2-3):121-40. Epub 2007 Dec 5. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18058037 18058037]
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
[[Category: Phosphoprotein phosphatase]]
[[Category: Large Structures]]
[[Category: Single protein]]
[[Category: Almo SC]]
[[Category: Almo, S C.]]
[[Category: Bain KT]]
[[Category: Bain, K T.]]
[[Category: Bonanno JB]]
[[Category: Bonanno, J B.]]
[[Category: Burley SK]]
[[Category: Burley, S K.]]
[[Category: Dickey M]]
[[Category: Dickey, M.]]
[[Category: Lau C]]
[[Category: Lau, C.]]
[[Category: Romero R]]
[[Category: NYSGXRC, New York Structural GenomiX Research Consortium.]]
[[Category: Sauder JM]]
[[Category: Romero, R.]]
[[Category: Smith D]]
[[Category: Sauder, J M.]]
[[Category: Wasserman S]]
[[Category: Smith, D.]]
[[Category: Wasserman, S.]]
[[Category: hydrolase]]
[[Category: new york structural genomix research consortium]]
[[Category: nysgxrc]]
[[Category: protein structure initiative]]
[[Category: psi-2]]
[[Category: structural genomic]]
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 04:44:58 2008''

Latest revision as of 12:14, 21 February 2024

Crystal structure of human carboxy-terminal domain RNA polymerase II polypeptide A small phosphatase 2Crystal structure of human carboxy-terminal domain RNA polymerase II polypeptide A small phosphatase 2

Structural highlights

2q5e is a 8 chain structure with sequence from Homo sapiens. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 2.51Å
Ligands:
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT, TOPSAN

Function

CTDS2_HUMAN Preferentially catalyzes the dephosphorylation of 'Ser-5' within the tandem 7 residues repeats in the C-terminal domain (CTD) of the largest RNA polymerase II subunit POLR2A. Negatively regulates RNA polymerase II transcription, possibly by controlling the transition from initiation/capping to processive transcript elongation. Recruited by REST to neuronal genes that contain RE-1 elements, leading to neuronal gene silencing in non-neuronal cells. May contribute to the development of sarcomas.[1] [2]

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

See Also

References

  1. ↑ Yeo M, Lin PS, Dahmus ME, Gill GN. A novel RNA polymerase II C-terminal domain phosphatase that preferentially dephosphorylates serine 5. J Biol Chem. 2003 Jul 11;278(28):26078-85. Epub 2003 Apr 28. PMID:12721286 doi:10.1074/jbc.M301791200
  2. ↑ Yeo M, Lee SK, Lee B, Ruiz EC, Pfaff SL, Gill GN. Small CTD phosphatases function in silencing neuronal gene expression. Science. 2005 Jan 28;307(5709):596-600. PMID:15681389 doi:10.1126/science.1100801

2q5e, resolution 2.51Å

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