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| [[Image:2q2k.jpg|left|200px]]
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| {{Structure
| | ==Structure of nucleic-acid binding protein== |
| |PDB= 2q2k |SIZE=350|CAPTION= <scene name='initialview01'>2q2k</scene>, resolution 3.00Å
| | <StructureSection load='2q2k' size='340' side='right'caption='[[2q2k]], [[Resolution|resolution]] 3.00Å' scene=''> |
| |SITE=
| | == Structural highlights == |
| |LIGAND= <scene name='pdbligand=5IU:5-IODO-2'-DEOXYURIDINE-5'-MONOPHOSPHATE'>5IU</scene>, <scene name='pdbligand=DA:2'-DEOXYADENOSINE-5'-MONOPHOSPHATE'>DA</scene>, <scene name='pdbligand=DC:2'-DEOXYCYTIDINE-5'-MONOPHOSPHATE'>DC</scene>, <scene name='pdbligand=DG:2'-DEOXYGUANOSINE-5'-MONOPHOSPHATE'>DG</scene>, <scene name='pdbligand=DT:THYMIDINE-5'-MONOPHOSPHATE'>DT</scene>, <scene name='pdbligand=EPE:4-(2-HYDROXYETHYL)-1-PIPERAZINE+ETHANESULFONIC+ACID'>EPE</scene>
| | <table><tr><td colspan='2'>[[2q2k]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2Q2K OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2Q2K FirstGlance]. <br> |
| |ACTIVITY=
| | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3Å</td></tr> |
| |GENE=
| | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=5IU:5-IODO-2-DEOXYURIDINE-5-MONOPHOSPHATE'>5IU</scene>, <scene name='pdbligand=EPE:4-(2-HYDROXYETHYL)-1-PIPERAZINE+ETHANESULFONIC+ACID'>EPE</scene></td></tr> |
| |DOMAIN=
| | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2q2k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2q2k OCA], [https://pdbe.org/2q2k PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2q2k RCSB], [https://www.ebi.ac.uk/pdbsum/2q2k PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2q2k ProSAT]</span></td></tr> |
| |RELATEDENTRY=
| | </table> |
| |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2q2k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2q2k OCA], [http://www.ebi.ac.uk/pdbsum/2q2k PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2q2k RCSB]</span>
| | == Function == |
| }}
| | [https://www.uniprot.org/uniprot/O87365_STAAU O87365_STAAU] |
| | | __TOC__ |
| '''Structure of nucleic-acid binding protein'''
| | </StructureSection> |
| | | [[Category: Large Structures]] |
| | |
| ==Overview==
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| The stable inheritance of genetic material depends on accurate DNA partition. Plasmids serve as tractable model systems to study DNA segregation because they require only a DNA centromere, a centromere-binding protein and a force-generating ATPase. The centromeres of partition (par) systems typically consist of a tandem arrangement of direct repeats. The best-characterized par system contains a centromere-binding protein called ParR and an ATPase called ParM. In the first step of segregation, multiple ParR proteins interact with the centromere repeats to form a large nucleoprotein complex of unknown structure called the segrosome, which binds ParM filaments. pSK41 ParR binds a centromere consisting of multiple 20-base-pair (bp) tandem repeats to mediate both transcription autoregulation and segregation. Here we report the structure of the pSK41 segrosome revealed in the crystal structure of a ParR-DNA complex. In the crystals, the 20-mer tandem repeats stack pseudo-continuously to generate the full-length centromere with the ribbon-helix-helix (RHH) fold of ParR binding successive DNA repeats as dimer-of-dimers. Remarkably, the dimer-of-dimers assemble in a continuous protein super-helical array, wrapping the DNA about its positive convex surface to form a large segrosome with an open, solenoid-shaped structure, suggesting a mechanism for ParM capture and subsequent plasmid segregation.
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| ==About this Structure==
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| 2Q2K is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2Q2K OCA].
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| ==Reference== | |
| Segrosome structure revealed by a complex of ParR with centromere DNA., Schumacher MA, Glover TC, Brzoska AJ, Jensen SO, Dunham TD, Skurray RA, Firth N, Nature. 2007 Dec 20;450(7173):1268-71. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18097417 18097417]
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| [[Category: Single protein]] | |
| [[Category: Staphylococcus aureus]] | | [[Category: Staphylococcus aureus]] |
| [[Category: Firth, N.]] | | [[Category: Firth N]] |
| [[Category: Glover, T.]] | | [[Category: Glover T]] |
| [[Category: Schumacher, M A.]] | | [[Category: Schumacher MA]] |
| [[Category: dna binding protein/dna complex]]
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| [[Category: parb]]
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| [[Category: partition]]
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| [[Category: protein-dna]]
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| [[Category: segregation]]
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| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 04:43:42 2008''
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