2pz8: Difference between revisions

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New page: left|200px<br /><applet load="2pz8" size="350" color="white" frame="true" align="right" spinBox="true" caption="2pz8, resolution 2.00Å" /> '''NAD+ Synthetase from...
 
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[[Image:2pz8.jpg|left|200px]]<br /><applet load="2pz8" size="350" color="white" frame="true" align="right" spinBox="true"
caption="2pz8, resolution 2.00&Aring;" />
'''NAD+ Synthetase from Bacillus anthracis with AMP-CPP and Mg2+'''<br />


==Overview==
==NAD+ Synthetase from Bacillus anthracis with AMP-CPP and Mg2+==
The crystal structures of NH(3)-dependent NAD(+) synthetase from Bacillus, anthracis as the apoenzyme (1.9 A), in complex with the natural catalytic, products AMP and pyrophosphate (2.4 A) and in complex with the substrate, analog adenosine 5'-(alpha,beta-methylene)triphosphate (2.0 A) have been, determined. NAD(+) synthetase catalyzes the last step in the biosynthesis, of the vitally important cofactor NAD(+). In comparison to other NAD(+), synthetase crystal structures, the C-terminal His-tagged end of the, apoenzyme adopts a novel helical conformation, causing significant, compensatory changes in the region. The structural accommodations observed, in B. anthracis NAD(+) synthetase are remarkable in the absence of adverse, affects on enzyme activity. They also illustrate a rare example of the, influence of a non-native C-terminal His-tag extension on the structure of, a native protein. In contrast to the apoenzyme, when AMP and pyrophosphate, or adenosine 5'-(alpha,beta-methylene)triphosphate are bound, the, C-terminus adopts a conformation that allows ATP binding and overall the, structure then resembles other NAD(+) synthetase structures. The, structures of NAD(+) synthetase complexes from B. anthracis are compared, with published X-ray crystal structures of the enzyme from B. subtilis, Escherichia coli and Helicobacter pylori. These comparisons support the, novel observation that P1 and P2 loop ordering is not a consequence of, crystal contacts but rather a consequence of intrinsic intramolecular, interactions within the ordered subunit.
<StructureSection load='2pz8' size='340' side='right'caption='[[2pz8]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[2pz8]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_anthracis Bacillus anthracis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PZ8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2PZ8 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=APC:DIPHOSPHOMETHYLPHOSPHONIC+ACID+ADENOSYL+ESTER'>APC</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2pz8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2pz8 OCA], [https://pdbe.org/2pz8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2pz8 RCSB], [https://www.ebi.ac.uk/pdbsum/2pz8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2pz8 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/NADE_BACAN NADE_BACAN]
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/pz/2pz8_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2pz8 ConSurf].
<div style="clear:both"></div>


==About this Structure==
==See Also==
2PZ8 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bacillus_anthracis Bacillus anthracis] with <scene name='pdbligand=MG:'>MG</scene>, <scene name='pdbligand=APC:'>APC</scene> and <scene name='pdbligand=GOL:'>GOL</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/NAD(+)_synthase NAD(+) synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.3.1.5 6.3.1.5] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PZ8 OCA].
*[[NAD synthase|NAD synthase]]
 
__TOC__
==Reference==
</StructureSection>
Structural adaptation of an interacting non-native C-terminal helical extension revealed in the crystal structure of NAD(+) synthetase from Bacillus anthracis., McDonald HM, Pruett PS, Deivanayagam C, Protasevich II, Carson WM, Delucas LJ, Brouillette WJ, Brouillette CG, Acta Crystallogr D Biol Crystallogr. 2007 Aug;63(Pt 8):891-905. Epub 2007, Jul 17. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17642516 17642516]
[[Category: Bacillus anthracis]]
[[Category: Bacillus anthracis]]
[[Category: NAD(+) synthase]]
[[Category: Large Structures]]
[[Category: Single protein]]
[[Category: Brouillette CG]]
[[Category: Brouillette, C.G.]]
[[Category: Brouillette WJ]]
[[Category: Brouillette, W.J.]]
[[Category: Carson WM]]
[[Category: Carson, W.M.]]
[[Category: DeLucas LJ]]
[[Category: DeLucas, L.J.]]
[[Category: Deivanayagam C]]
[[Category: Deivanayagam, C.]]
[[Category: McDonald HM]]
[[Category: McDonald, H.M.]]
[[Category: Protasevich II]]
[[Category: Protasevich, I.I.]]
[[Category: Pruett PS]]
[[Category: Pruett, P.S.]]
[[Category: APC]]
[[Category: GOL]]
[[Category: MG]]
[[Category: his-tag]]
[[Category: ligase]]
[[Category: protein-substrate analog complex]]
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jan 23 13:49:53 2008''

Latest revision as of 12:13, 21 February 2024

NAD+ Synthetase from Bacillus anthracis with AMP-CPP and Mg2+NAD+ Synthetase from Bacillus anthracis with AMP-CPP and Mg2+

Structural highlights

2pz8 is a 2 chain structure with sequence from Bacillus anthracis. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 2Å
Ligands:, ,
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

NADE_BACAN

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

See Also

2pz8, resolution 2.00Å

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