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[[Image:2px0.jpg|left|200px]]


{{Structure
==Crystal structure of FlhF complexed with GMPPNP/Mg(2+)==
|PDB= 2px0 |SIZE=350|CAPTION= <scene name='initialview01'>2px0</scene>, resolution 3.00&Aring;
<StructureSection load='2px0' size='340' side='right'caption='[[2px0]], [[Resolution|resolution]] 3.00&Aring;' scene=''>
|SITE=  
== Structural highlights ==
|LIGAND= <scene name='pdbligand=GNP:PHOSPHOAMINOPHOSPHONIC+ACID-GUANYLATE+ESTER'>GNP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>
<table><tr><td colspan='2'>[[2px0]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PX0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2PX0 FirstGlance]. <br>
|ACTIVITY=  
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3&#8491;</td></tr>
|GENE= flhF ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1423 Bacillus subtilis])
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GNP:PHOSPHOAMINOPHOSPHONIC+ACID-GUANYLATE+ESTER'>GNP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
|DOMAIN=
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2px0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2px0 OCA], [https://pdbe.org/2px0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2px0 RCSB], [https://www.ebi.ac.uk/pdbsum/2px0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2px0 ProSAT]</span></td></tr>
|RELATEDENTRY=[[2px3|2PX3]]
</table>
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2px0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2px0 OCA], [http://www.ebi.ac.uk/pdbsum/2px0 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2px0 RCSB]</span>
== Function ==
}}
[https://www.uniprot.org/uniprot/FLHF_BACSU FLHF_BACSU] Necessary for flagellar biosynthesis. May be involved in translocation of the flagellum.
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/px/2px0_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2px0 ConSurf].
<div style="clear:both"></div>


'''Crystal structure of FlhF complexed with GMPPNP/Mg(2+)'''
==See Also==
 
*[[Flagellar biosynthetic protein|Flagellar biosynthetic protein]]
 
*[[Flagellar protein 3D structures|Flagellar protein 3D structures]]
==Overview==
*[[GTP-binding protein 3D structures|GTP-binding protein 3D structures]]
Flagella are well characterized as the organelles of locomotion and allow bacteria to react to environmental changes. The assembly of flagella is a multistep process and relies on a complex type III export machinery located in the cytoplasmic membrane. The FlhF protein is essential for the placement and assembly of polar flagella and has been classified as a signal-recognition particle (SRP)-type GTPase. SRP GTPases appeared early in evolution and form a unique subfamily within the guanine nucleotide binding proteins with only three members: the signal sequence-binding protein SRP54, the SRP receptor FtsY, and FlhF. We report the crystal structures of FlhF from Bacillus subtilis in complex with GTP and GMPPNP. FlhF shares SRP GTPase-specific features such as the presence of an N-terminal alpha-helical domain and the I-box insertion. It forms a symmetric homodimer sequestering a composite active site that contains two head-to-tail arranged nucleotides similar to the heterodimeric SRP-targeting complex. However, significant differences to the GTPases of SRP and the SRP receptor include the formation of a stable homodimer with GTP as well as severe modifications and even the absence of motifs involved in regulation of the other two SRP GTPases. Our results provide insights into SRP GTPases and their roles in two fundamentally different protein-targeting routes that both rely on efficient protein delivery to a secretion channel.
__TOC__
 
</StructureSection>
==About this Structure==
2PX0 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PX0 OCA].
 
==Reference==
The crystal structure of the third signal-recognition particle GTPase FlhF reveals a homodimer with bound GTP., Bange G, Petzold G, Wild K, Parlitz RO, Sinning I, Proc Natl Acad Sci U S A. 2007 Aug 21;104(34):13621-5. Epub 2007 Aug 15. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17699634 17699634]
[[Category: Bacillus subtilis]]
[[Category: Bacillus subtilis]]
[[Category: Single protein]]
[[Category: Large Structures]]
[[Category: Bange, G.]]
[[Category: Bange G]]
[[Category: Sinning, I.]]
[[Category: Sinning I]]
[[Category: Wild, K.]]
[[Category: Wild K]]
[[Category: biosynthetic protein]]
[[Category: flagellum]]
[[Category: protein transport]]
[[Category: srp gtpase]]
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 04:41:49 2008''

Latest revision as of 12:12, 21 February 2024

Crystal structure of FlhF complexed with GMPPNP/Mg(2+)Crystal structure of FlhF complexed with GMPPNP/Mg(2+)

Structural highlights

2px0 is a 8 chain structure with sequence from Bacillus subtilis. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 3Å
Ligands:,
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

FLHF_BACSU Necessary for flagellar biosynthesis. May be involved in translocation of the flagellum.

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

See Also

2px0, resolution 3.00Å

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OCA
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