2px0: Difference between revisions

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-[[Image:2px0.jpg|left|200px]]<br /><applet load="2px0" size="350" color="white" frame="true" align="right" spinBox="true"
-caption="2px0, resolution 3.00&Aring;" />
-'''Crystal structure of FlhF complexed with GMPPNP/Mg(2+)'''<br />
-==Overview==
+==Crystal structure of FlhF complexed with GMPPNP/Mg(2+)==
-Flagella are well characterized as the organelles of locomotion and allow bacteria to react to environmental changes. The assembly of flagella is a multistep process and relies on a complex type III export machinery located in the cytoplasmic membrane. The FlhF protein is essential for the placement and assembly of polar flagella and has been classified as a signal-recognition particle (SRP)-type GTPase. SRP GTPases appeared early in evolution and form a unique subfamily within the guanine nucleotide binding proteins with only three members: the signal sequence-binding protein SRP54, the SRP receptor FtsY, and FlhF. We report the crystal structures of FlhF from Bacillus subtilis in complex with GTP and GMPPNP. FlhF shares SRP GTPase-specific features such as the presence of an N-terminal alpha-helical domain and the I-box insertion. It forms a symmetric homodimer sequestering a composite active site that contains two head-to-tail arranged nucleotides similar to the heterodimeric SRP-targeting complex. However, significant differences to the GTPases of SRP and the SRP receptor include the formation of a stable homodimer with GTP as well as severe modifications and even the absence of motifs involved in regulation of the other two SRP GTPases. Our results provide insights into SRP GTPases and their roles in two fundamentally different protein-targeting routes that both rely on efficient protein delivery to a secretion channel.
+<StructureSection load='2px0' size='340' side='right'caption='[[2px0]], [[Resolution|resolution]] 3.00&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[2px0]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PX0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2PX0 FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3&#8491;</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GNP:PHOSPHOAMINOPHOSPHONIC+ACID-GUANYLATE+ESTER'>GNP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2px0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2px0 OCA], [https://pdbe.org/2px0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2px0 RCSB], [https://www.ebi.ac.uk/pdbsum/2px0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2px0 ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/FLHF_BACSU FLHF_BACSU] Necessary for flagellar biosynthesis. May be involved in translocation of the flagellum.
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/px/2px0_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2px0 ConSurf].
+<div style="clear:both"></div>
-==About this Structure==
+==See Also==
-PX0 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis] with <scene name='pdbligand=MG:'>MG</scene> and <scene name='pdbligand=GNP:'>GNP</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PX0 OCA].
+*[[Flagellar biosynthetic protein|Flagellar biosynthetic protein]]
+*[[Flagellar protein 3D structures|Flagellar protein 3D structures]]
-==Reference==
+*[[GTP-binding protein 3D structures|GTP-binding protein 3D structures]]
-The crystal structure of the third signal-recognition particle GTPase FlhF reveals a homodimer with bound GTP., Bange G, Petzold G, Wild K, Parlitz RO, Sinning I, Proc Natl Acad Sci U S A. 2007 Aug 21;104(34):13621-5. Epub 2007 Aug 15. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17699634 17699634]
+__TOC__
+</StructureSection>
 [[Category: Bacillus subtilis]]
-[[Category: Single protein]]
+[[Category: Large Structures]]
-[[Category: Bange, G.]]
+[[Category: Bange G]]
-[[Category: Sinning, I.]]
+[[Category: Sinning I]]
-[[Category: Wild, K.]]
+[[Category: Wild K]]
-[[Category: GNP]]
-[[Category: MG]]
-[[Category: biosynthetic protein]]
-[[Category: flagellum]]
-[[Category: protein transport]]
-[[Category: srp gtpase]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 18:33:50 2008''