2psf: Difference between revisions

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[[Image:2psf.gif|left|200px]]


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==Crystal Structures of the Luciferase and Green Fluorescent Protein from Renilla Reniformis==
The line below this paragraph, containing "STRUCTURE_2psf", creates the "Structure Box" on the page.
<StructureSection load='2psf' size='340' side='right'caption='[[2psf]], [[Resolution|resolution]] 1.40&Aring;' scene=''>
You may change the PDB parameter (which sets the PDB file loaded into the applet)
== Structural highlights ==
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
<table><tr><td colspan='2'>[[2psf]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Renilla_reniformis Renilla reniformis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PSF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2PSF FirstGlance]. <br>
or leave the SCENE parameter empty for the default display.
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.4&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2psf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2psf OCA], [https://pdbe.org/2psf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2psf RCSB], [https://www.ebi.ac.uk/pdbsum/2psf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2psf ProSAT]</span></td></tr>
{{STRUCTURE_2psf|  PDB=2psf |  SCENE= }}
</table>
== Function ==
[https://www.uniprot.org/uniprot/LUCI_RENRE LUCI_RENRE]
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ps/2psf_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2psf ConSurf].
<div style="clear:both"></div>


'''Crystal Structures of the Luciferase and Green Fluorescent Protein from Renilla Reniformis'''
==See Also==
 
*[[Luciferase 3D structures|Luciferase 3D structures]]
 
__TOC__
==Overview==
</StructureSection>
Due to its ability to emit light, the luciferase from Renilla reniformis (RLuc) is widely employed in molecular biology as a reporter gene in cell culture experiments and small animal imaging. To accomplish this bioluminescence, the 37-kDa enzyme catalyzes the degradation of its substrate coelenterazine in the presence of molecular oxygen, resulting in the product coelenteramide, carbon dioxide, and the desired photon of light. We successfully crystallized a stabilized variant of this important protein (RLuc8) and herein present the first structures for any coelenterazine-using luciferase. These structures are based on high-resolution data measured to 1.4 A and demonstrate a classic alpha/beta-hydrolase fold. We also present data of a coelenteramide-bound luciferase and reason that this structure represents a secondary conformational form following shift of the product out of the primary active site. During the course of this work, the structure of the luciferase's accessory green fluorescent protein (RrGFP) was also determined and shown to be highly similar to that of Aequorea victoria GFP.
[[Category: Large Structures]]
 
==About this Structure==
2PSF is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Renilla_reniformis Renilla reniformis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PSF OCA].
 
==Reference==
Crystal structures of the luciferase and green fluorescent protein from Renilla reniformis., Loening AM, Fenn TD, Gambhir SS, J Mol Biol. 2007 Dec 7;374(4):1017-28. Epub 2007 Oct 3. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17980388 17980388]
[[Category: Renilla reniformis]]
[[Category: Renilla reniformis]]
[[Category: Renilla-luciferin 2-monooxygenase]]
[[Category: Fenn TD]]
[[Category: Single protein]]
[[Category: Gambhir SS]]
[[Category: Fenn, T D.]]
[[Category: Loening AM]]
[[Category: Gambhir, S S.]]
[[Category: Loening, A M.]]
[[Category: Alpha/beta-hydrolase]]
[[Category: Luciferase]]
[[Category: Oxidoreductase]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May  4 13:43:41 2008''

Latest revision as of 12:11, 21 February 2024

Crystal Structures of the Luciferase and Green Fluorescent Protein from Renilla ReniformisCrystal Structures of the Luciferase and Green Fluorescent Protein from Renilla Reniformis

Structural highlights

2psf is a 2 chain structure with sequence from Renilla reniformis. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 1.4Å
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

LUCI_RENRE

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

See Also

2psf, resolution 1.40Å

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