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| [[Image:2pkn.jpg|left|200px]]<br /><applet load="2pkn" size="350" color="white" frame="true" align="right" spinBox="true"
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| caption="2pkn, resolution 1.90Å" />
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| '''Crystal structure of M tuberculosis Adenosine Kinase complexed with AMP-PCP (non-hydrolyzable ATP analog)'''<br />
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| ==Overview== | | ==Crystal structure of M tuberculosis Adenosine Kinase complexed with AMP-PCP (non-hydrolyzable ATP analog)== |
| Adenosine kinase (ADK) catalyzes the phosphorylation of adenosine (Ado) to, adenosine monophosphate (AMP). It is part of the purine salvage pathway, that has been identified only in eukaryotes, with the single exception of, Mycobacterium spp. Whereas it is not clear if Mycobacterium tuberculosis, (Mtb) ADK is essential, it has been shown that the enzyme can selectively, phosphorylate nucleoside analogs to produce products toxic to the cell. We, have determined the crystal structure of Mtb ADK unliganded as well as, ligand (Ado) bound at 1.5- and 1.9-A resolution, respectively. The, structure of the binary complexes with the inhibitor 2-fluoroadenosine, (F-Ado) bound and with the adenosine 5'-(beta,gamma-methylene)triphosphate, (AMP-PCP) (non-hydrolyzable ATP analog) bound were also solved at 1.9-A, resolution. These four structures indicate that Mtb ADK is a dimer formed, by an extended beta sheet. The active site of the unliganded ADK is in an, open conformation, and upon Ado binding a lid domain of the protein, undergoes a large conformation change to close the active site. In the, closed conformation, the lid forms direct interactions with the substrate, and residues of the active site. Interestingly, AMP-PCP binding alone was, not sufficient to produce the closed state of the enzyme. The binding mode, of F-Ado was characterized to illustrate the role of additional, non-bonding interactions in Mtb ADK compared with human ADK.
| | <StructureSection load='2pkn' size='340' side='right'caption='[[2pkn]], [[Resolution|resolution]] 1.90Å' scene=''> |
| | == Structural highlights == |
| | <table><tr><td colspan='2'>[[2pkn]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PKN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2PKN FirstGlance]. <br> |
| | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9Å</td></tr> |
| | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACP:PHOSPHOMETHYLPHOSPHONIC+ACID+ADENYLATE+ESTER'>ACP</scene></td></tr> |
| | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2pkn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2pkn OCA], [https://pdbe.org/2pkn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2pkn RCSB], [https://www.ebi.ac.uk/pdbsum/2pkn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2pkn ProSAT]</span></td></tr> |
| | </table> |
| | == Function == |
| | [https://www.uniprot.org/uniprot/ADOK_MYCTU ADOK_MYCTU] ATP dependent phosphorylation of adenosine to monophosphate derivatives (By similarity). |
| | == Evolutionary Conservation == |
| | [[Image:Consurf_key_small.gif|200px|right]] |
| | Check<jmol> |
| | <jmolCheckbox> |
| | <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/pk/2pkn_consurf.spt"</scriptWhenChecked> |
| | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> |
| | <text>to colour the structure by Evolutionary Conservation</text> |
| | </jmolCheckbox> |
| | </jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2pkn ConSurf]. |
| | <div style="clear:both"></div> |
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| ==About this Structure== | | ==See Also== |
| 2PKN is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis] with <scene name='pdbligand=ACP:'>ACP</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Adenosine_kinase Adenosine kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.20 2.7.1.20] Known structural/functional Site: <scene name='pdbsite=AC1:Acp+Binding+Site+For+Residue+A+325'>AC1</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PKN OCA].
| | *[[Adenosine kinase 3D structures|Adenosine kinase 3D structures]] |
| | | __TOC__ |
| ==Reference==
| | </StructureSection> |
| High resolution crystal structures of Mycobacterium tuberculosis adenosine kinase: insights into the mechanism and specificity of this novel prokaryotic enzyme., Reddy MC, Palaninathan SK, Shetty ND, Owen JL, Watson MD, Sacchettini JC, J Biol Chem. 2007 Sep 14;282(37):27334-42. Epub 2007 Jun 26. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17597075 17597075]
| | [[Category: Large Structures]] |
| [[Category: Adenosine kinase]] | |
| [[Category: Mycobacterium tuberculosis]] | | [[Category: Mycobacterium tuberculosis]] |
| [[Category: Single protein]]
| | [[Category: Owen JL]] |
| [[Category: Owen, J.L.]] | | [[Category: Palaninathan SK]] |
| [[Category: Palaninathan, S.K.]] | | [[Category: Reddy MCM]] |
| [[Category: Reddy, M.C.M.]] | | [[Category: Sacchettini JC]] |
| [[Category: Sacchettini, J.C.]] | | [[Category: Shetty ND]] |
| [[Category: Shetty, N.D.]] | | [[Category: Watson MD]] |
| [[Category: TBSGC, TB.Structural.Genomics.Consortium.]]
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| [[Category: Watson, M.D.]] | |
| [[Category: ACP]]
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| [[Category: adenosine kinase]]
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| [[Category: atp]]
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| [[Category: mycobacterium tuberculosis]]
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| [[Category: structural genomics]]
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| [[Category: tb structural genomics consortium]]
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| [[Category: tbsgc]]
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| [[Category: transferase]]
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| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Feb 13 08:18:00 2008''
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