2p6r: Difference between revisions

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[[Image:2p6r.gif|left|200px]]


{{Structure
==Crystal structure of superfamily 2 helicase Hel308 in complex with unwound DNA==
|PDB= 2p6r |SIZE=350|CAPTION= <scene name='initialview01'>2p6r</scene>, resolution 3.000&Aring;
<StructureSection load='2p6r' size='340' side='right'caption='[[2p6r]], [[Resolution|resolution]] 3.00&Aring;' scene=''>
|SITE=  
== Structural highlights ==
|LIGAND=  
<table><tr><td colspan='2'>[[2p6r]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Archaeoglobus_fulgidus Archaeoglobus fulgidus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2P6R OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2P6R FirstGlance]. <br>
|ACTIVITY=  
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3&#8491;</td></tr>
|GENE=  
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2p6r FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2p6r OCA], [https://pdbe.org/2p6r PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2p6r RCSB], [https://www.ebi.ac.uk/pdbsum/2p6r PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2p6r ProSAT]</span></td></tr>
}}
</table>
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/p6/2p6r_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2p6r ConSurf].
<div style="clear:both"></div>


'''Crystal structure of superfamily 2 helicase Hel308 in complex with unwound DNA'''
==See Also==
 
*[[Helicase 3D structures|Helicase 3D structures]]
 
__TOC__
==Overview==
</StructureSection>
To reveal the mechanism of processive strand separation by superfamily-2 (SF2) 3'--&gt;5' helicases, we determined apo and DNA-bound crystal structures of archaeal Hel308, a helicase that unwinds lagging strands and is related to human DNA polymerase theta. Our structure captures the duplex-unwinding reaction, shows that initial strand separation does not require ATP and identifies a prominent beta-hairpin loop as the unwinding element. Similar loops in hepatitis C virus NS3 helicase and RNA-decay factors support the idea that this duplex-unwinding mechanism is applicable to a broad subset of SF2 helicases. Comparison with ATP-bound SF2 enzymes suggests that ATP promotes processive unwinding of 1 base pair by ratchet-like transport of the 3' product strand. Our results provide a first structural framework for strand separation by processive SF2 3'--&gt;5' helicases and reveal important mechanistic differences from SF1 helicases.
 
==About this Structure==
2P6R is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Archaeoglobus_fulgidus Archaeoglobus fulgidus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2P6R OCA].
 
==Reference==
Structural basis for DNA duplex separation by a superfamily-2 helicase., Buttner K, Nehring S, Hopfner KP, Nat Struct Mol Biol. 2007 Jul;14(7):647-52. Epub 2007 Jun 10. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17558417 17558417]
[[Category: Archaeoglobus fulgidus]]
[[Category: Archaeoglobus fulgidus]]
[[Category: Protein complex]]
[[Category: Large Structures]]
[[Category: Buettner, K.]]
[[Category: Buettner K]]
[[Category: Hopfner, K P.]]
[[Category: Hopfner KP]]
[[Category: Nehring, S.]]
[[Category: Nehring S]]
[[Category: archaeal helicase]]
[[Category: dna repair]]
[[Category: protein-dna complex]]
[[Category: sf2 helicase]]
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 18:09:23 2008''

Latest revision as of 12:08, 21 February 2024

Crystal structure of superfamily 2 helicase Hel308 in complex with unwound DNACrystal structure of superfamily 2 helicase Hel308 in complex with unwound DNA

Structural highlights

2p6r is a 3 chain structure with sequence from Archaeoglobus fulgidus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 3Å
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

See Also

2p6r, resolution 3.00Å

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Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA
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