2p6r: Difference between revisions

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-[[Image:2p6r.gif|left|200px]]<br /><applet load="2p6r" size="350" color="white" frame="true" align="right" spinBox="true"
-caption="2p6r, resolution 3.000&Aring;" />
-'''Crystal structure of superfamily 2 helicase Hel308 in complex with unwound DNA'''<br />
-==Overview==
+==Crystal structure of superfamily 2 helicase Hel308 in complex with unwound DNA==
-To reveal the mechanism of processive strand separation by superfamily-2 (SF2) 3'--&gt;5' helicases, we determined apo and DNA-bound crystal structures of archaeal Hel308, a helicase that unwinds lagging strands and is related to human DNA polymerase theta. Our structure captures the duplex-unwinding reaction, shows that initial strand separation does not require ATP and identifies a prominent beta-hairpin loop as the unwinding element. Similar loops in hepatitis C virus NS3 helicase and RNA-decay factors support the idea that this duplex-unwinding mechanism is applicable to a broad subset of SF2 helicases. Comparison with ATP-bound SF2 enzymes suggests that ATP promotes processive unwinding of 1 base pair by ratchet-like transport of the 3' product strand. Our results provide a first structural framework for strand separation by processive SF2 3'--&gt;5' helicases and reveal important mechanistic differences from SF1 helicases.
+<StructureSection load='2p6r' size='340' side='right'caption='[[2p6r]], [[Resolution|resolution]] 3.00&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[2p6r]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Archaeoglobus_fulgidus Archaeoglobus fulgidus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2P6R OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2P6R FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3&#8491;</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2p6r FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2p6r OCA], [https://pdbe.org/2p6r PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2p6r RCSB], [https://www.ebi.ac.uk/pdbsum/2p6r PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2p6r ProSAT]</span></td></tr>
+</table>
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/p6/2p6r_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2p6r ConSurf].
+<div style="clear:both"></div>
-==About this Structure==
+==See Also==
-P6R is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Archaeoglobus_fulgidus Archaeoglobus fulgidus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2P6R OCA].
+*[[Helicase 3D structures|Helicase 3D structures]]
+__TOC__
-==Reference==
+</StructureSection>
-Structural basis for DNA duplex separation by a superfamily-2 helicase., Buttner K, Nehring S, Hopfner KP, Nat Struct Mol Biol. 2007 Jul;14(7):647-52. Epub 2007 Jun 10. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17558417 17558417]
 [[Category: Archaeoglobus fulgidus]]
-[[Category: Protein complex]]
+[[Category: Large Structures]]
-[[Category: Buettner, K.]]
+[[Category: Buettner K]]
-[[Category: Hopfner, K P.]]
+[[Category: Hopfner KP]]
-[[Category: Nehring, S.]]
+[[Category: Nehring S]]
-[[Category: archaeal helicase]]
-[[Category: dna repair]]
-[[Category: protein-dna complex]]
-[[Category: sf2 helicase]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 18:26:26 2008''