2orw: Difference between revisions

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-[[Image:2orw.jpg|left|200px]]
- {{Structure
+==Thermotoga maritima thymidine kinase 1 like enzyme in complex with TP4A==
-|PDB= 2orw |SIZE=350|CAPTION= <scene name='initialview01'>2orw</scene>, resolution 1.500&Aring;
+<StructureSection load='2orw' size='340' side='right'caption='[[2orw]], [[Resolution|resolution]] 1.50&Aring;' scene=''>
-|SITE=
+== Structural highlights ==
-|LIGAND= <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene> and <scene name='pdbligand=4TA:P1-(5&#39;-ADENOSYL)P4-(5&#39;-(2&#39;-DEOXY-THYMIDYL))TETRAPHOSPHATE'>4TA</scene>
+<table><tr><td colspan='2'>[[2orw]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermotoga_maritima Thermotoga maritima]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ORW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2ORW FirstGlance]. <br>
-|ACTIVITY= [http://en.wikipedia.org/wiki/Thymidine_kinase Thymidine kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.21 2.7.1.21]
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.5&#8491;</td></tr>
-|GENE= tdk ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=2336 Thermotoga maritima])
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=4TA:P1-(5-ADENOSYL)P4-(5-(2-DEOXY-THYMIDYL))TETRAPHOSPHATE'>4TA</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-}}
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2orw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2orw OCA], [https://pdbe.org/2orw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2orw RCSB], [https://www.ebi.ac.uk/pdbsum/2orw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2orw ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/KITH_THEMA KITH_THEMA]
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/or/2orw_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2orw ConSurf].
+<div style="clear:both"></div>
-'''Thermotoga maritima thymidine kinase 1 like enzyme in complex with TP4A'''
+==See Also==
+*[[Thymidine kinase 3D structures|Thymidine kinase 3D structures]]
+__TOC__
-==Overview==
+</StructureSection>
-Human thymidine kinase 1 (hTK1) and structurally related TKs from other organisms catalyze the initial phosphorylation step in the thymidine salvage pathway. Though ATP is known to be the preferred phosphoryl donor for TK1-like enzymes, its exact binding mode and effect on the oligomeric state has not been analyzed. Here we report the structures of hTK1 and of the Thermotoga maritima thymidine kinase (TmTK) in complex with the bisubstrate inhibitor TP4A. The TmTK-TP4A structure reveals that the adenosine moiety of ATP binds at the subunit interface of the homotetrameric enzyme and that the majority of the ATP-enzyme interactions occur between the phosphate groups and the P-loop. In the hTK1 structure the adenosine group of TP4A exhibited no electron density. This difference between hTK1 and TmTK is rationalized by a difference in the conformation of their quaternary structure. A more open conformation, as seen in the TmTK-TP4A complex structure, is required to provide space for the adenosine moiety. Our analysis supports the formation of an analogous open conformation in hTK1 upon ATP binding.
+[[Category: Large Structures]]
-==About this Structure==
-ORW is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Thermotoga_maritima Thermotoga maritima]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ORW OCA].
-==Reference==
-Binding of ATP to TK1-like enzymes is associated with a conformational change in the quaternary structure., Segura-Pena D, Lutz S, Monnerjahn C, Konrad M, Lavie A, J Mol Biol. 2007 May 25;369(1):129-41. Epub 2007 Mar 15. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17407781 17407781]
-[[Category: Single protein]]
 [[Category: Thermotoga maritima]]
-[[Category: Thymidine kinase]]
+[[Category: Konrad M]]
-[[Category: Konrad, M.]]
+[[Category: Lavie A]]
-[[Category: Lavie, A.]]
+[[Category: Lutz S]]
-[[Category: Lutz, S.]]
+[[Category: Monnerjahn C]]
-[[Category: Monnerjahn, C.]]
+[[Category: Segura-Pena D]]
-[[Category: Segura-Pena, D.]]
-[[Category: 4TA]]
-[[Category: MG]]
-[[Category: ZN]]
-[[Category: tmtk]]
-[[Category: tp4a]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 23 15:34:35 2008''