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| [[Image:1k09.jpg|left|200px]]
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| {{Structure
| | ==Solution structure of BetaCore, A Designed Water Soluble Four-Stranded Antiparallel b-sheet Protein== |
| |PDB= 1k09 |SIZE=350|CAPTION= <scene name='initialview01'>1k09</scene>
| | <StructureSection load='1k09' size='340' side='right'caption='[[1k09]]' scene=''> |
| |SITE= | | == Structural highlights == |
| |LIGAND= <scene name='pdbligand=ABA:ALPHA-AMINOBUTYRIC+ACID'>ABA</scene>, <scene name='pdbligand=CLG:2-AMINO-6-[2-(2-AMINOOXY-ACETYLAMINO)-ACETYLAMINO]-HEXANOIC+ACID'>CLG</scene>, <scene name='pdbligand=CLH:2-AMINO-6-[2-(2-OXO-ACETYLAMINO)-ACETYLAMINO]-HEXANOIC+ACID'>CLH</scene>, <scene name='pdbligand=MPT:BETA-MERCAPTOPROPIONIC+ACID'>MPT</scene>, <scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene> | | <table><tr><td colspan='2'>[[1k09]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1K09 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1K09 FirstGlance]. <br> |
| |ACTIVITY=
| | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr> |
| |GENE=
| | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ABA:ALPHA-AMINOBUTYRIC+ACID'>ABA</scene>, <scene name='pdbligand=CLG:2-AMINO-6-[2-(2-AMINOOXY-ACETYLAMINO)-ACETYLAMINO]-HEXANOIC+ACID'>CLG</scene>, <scene name='pdbligand=CLH:2-AMINO-6-[2-(2-OXO-ACETYLAMINO)-ACETYLAMINO]-HEXANOIC+ACID'>CLH</scene>, <scene name='pdbligand=MPT:BETA-MERCAPTOPROPIONIC+ACID'>MPT</scene>, <scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene></td></tr> |
| |DOMAIN=
| | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1k09 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1k09 OCA], [https://pdbe.org/1k09 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1k09 RCSB], [https://www.ebi.ac.uk/pdbsum/1k09 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1k09 ProSAT]</span></td></tr> |
| |RELATEDENTRY=
| | </table> |
| |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1k09 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1k09 OCA], [http://www.ebi.ac.uk/pdbsum/1k09 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1k09 RCSB]</span>
| | == Function == |
| }}
| | [https://www.uniprot.org/uniprot/BPT1_BOVIN BPT1_BOVIN] Inhibits trypsin, kallikrein, chymotrypsin, and plasmin. |
| | | __TOC__ |
| '''Solution structure of BetaCore, A Designed Water Soluble Four-Stranded Antiparallel b-sheet Protein'''
| | </StructureSection> |
| | | [[Category: Bos taurus]] |
| | | [[Category: Large Structures]] |
| ==Overview==
| | [[Category: Barany G]] |
| BetaCore is a designed approximately 50-residue protein in which two BPTI-derived core modules, CM I and CM II, are connected by a 22-atom cross-link. At low temperature and pH 3, homo- and heteronuclear NMR data report a dominant folded ('f') conformation with well-dispersed chemical shifts, i, i+1 periodicity, numerous long-range NOEs, and slowed amide hydrogen isotope exchange patterns that is a four-stranded antiparallel beta-sheet with nonsymmetrical and specific association of CM I and CM II. BetaCore 'f' conformations undergo reversible, global, moderately cooperative, non-two-state thermal transitions to an equilibrium ensemble of unfolded 'u' conformations. There is a significant energy barrier between 'f' and 'u' conformations. This is the first designed four-stranded antiparallel beta-sheet that folds in water.
| | [[Category: Carulla N]] |
| | | [[Category: Woodward C]] |
| ==About this Structure==
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| 1K09 is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1K09 OCA].
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| ==Reference== | |
| BetaCore, a designed water soluble four-stranded antiparallel beta-sheet protein., Carulla N, Woodward C, Barany G, Protein Sci. 2002 Jun;11(6):1539-51. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12021452 12021452]
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| [[Category: Protein complex]] | |
| [[Category: Barany, G.]] | |
| [[Category: Carulla, N.]] | |
| [[Category: Woodward, C.]] | |
| [[Category: four-stranded antiparallel beta-sheet]] | |
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| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 21:41:31 2008''
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