1k09: Difference between revisions

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==Solution structure of BetaCore, A Designed Water Soluble Four-Stranded Antiparallel b-sheet Protein==
==Solution structure of BetaCore, A Designed Water Soluble Four-Stranded Antiparallel b-sheet Protein==
<StructureSection load='1k09' size='340' side='right' caption='[[1k09]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''>
<StructureSection load='1k09' size='340' side='right'caption='[[1k09]]' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[1k09]] is a 2 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1K09 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1K09 FirstGlance]. <br>
<table><tr><td colspan='2'>[[1k09]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1K09 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1K09 FirstGlance]. <br>
</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=ABA:ALPHA-AMINOBUTYRIC+ACID'>ABA</scene>, <scene name='pdbligand=CLG:2-AMINO-6-[2-(2-AMINOOXY-ACETYLAMINO)-ACETYLAMINO]-HEXANOIC+ACID'>CLG</scene>, <scene name='pdbligand=CLH:2-AMINO-6-[2-(2-OXO-ACETYLAMINO)-ACETYLAMINO]-HEXANOIC+ACID'>CLH</scene>, <scene name='pdbligand=MPT:BETA-MERCAPTOPROPIONIC+ACID'>MPT</scene>, <scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1k09 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1k09 OCA], [http://pdbe.org/1k09 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1k09 RCSB], [http://www.ebi.ac.uk/pdbsum/1k09 PDBsum]</span></td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ABA:ALPHA-AMINOBUTYRIC+ACID'>ABA</scene>, <scene name='pdbligand=CLG:2-AMINO-6-[2-(2-AMINOOXY-ACETYLAMINO)-ACETYLAMINO]-HEXANOIC+ACID'>CLG</scene>, <scene name='pdbligand=CLH:2-AMINO-6-[2-(2-OXO-ACETYLAMINO)-ACETYLAMINO]-HEXANOIC+ACID'>CLH</scene>, <scene name='pdbligand=MPT:BETA-MERCAPTOPROPIONIC+ACID'>MPT</scene>, <scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1k09 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1k09 OCA], [https://pdbe.org/1k09 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1k09 RCSB], [https://www.ebi.ac.uk/pdbsum/1k09 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1k09 ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
[[http://www.uniprot.org/uniprot/BPT1_BOVIN BPT1_BOVIN]] Inhibits trypsin, kallikrein, chymotrypsin, and plasmin.  
[https://www.uniprot.org/uniprot/BPT1_BOVIN BPT1_BOVIN] Inhibits trypsin, kallikrein, chymotrypsin, and plasmin.
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
BetaCore is a designed approximately 50-residue protein in which two BPTI-derived core modules, CM I and CM II, are connected by a 22-atom cross-link. At low temperature and pH 3, homo- and heteronuclear NMR data report a dominant folded ('f') conformation with well-dispersed chemical shifts, i, i+1 periodicity, numerous long-range NOEs, and slowed amide hydrogen isotope exchange patterns that is a four-stranded antiparallel beta-sheet with nonsymmetrical and specific association of CM I and CM II. BetaCore 'f' conformations undergo reversible, global, moderately cooperative, non-two-state thermal transitions to an equilibrium ensemble of unfolded 'u' conformations. There is a significant energy barrier between 'f' and 'u' conformations. This is the first designed four-stranded antiparallel beta-sheet that folds in water.
 
BetaCore, a designed water soluble four-stranded antiparallel beta-sheet protein.,Carulla N, Woodward C, Barany G Protein Sci. 2002 Jun;11(6):1539-51. PMID:12021452<ref>PMID:12021452</ref>
 
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 1k09" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Barany, G]]
[[Category: Bos taurus]]
[[Category: Carulla, N]]
[[Category: Large Structures]]
[[Category: Woodward, C]]
[[Category: Barany G]]
[[Category: De novo protein]]
[[Category: Carulla N]]
[[Category: Four-stranded antiparallel beta-sheet]]
[[Category: Woodward C]]

Latest revision as of 12:00, 21 February 2024

Solution structure of BetaCore, A Designed Water Soluble Four-Stranded Antiparallel b-sheet ProteinSolution structure of BetaCore, A Designed Water Soluble Four-Stranded Antiparallel b-sheet Protein

Structural highlights

1k09 is a 2 chain structure with sequence from Bos taurus. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:Solution NMR
Ligands:, , , ,
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

BPT1_BOVIN Inhibits trypsin, kallikrein, chymotrypsin, and plasmin.

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