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| [[Image:3boe.jpg|left|200px]]
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| {{Structure
| | ==Carbonic anhydrase from marine diatom Thalassiosira weissflogii- cadmium bound domain 2 with acetate (CDCA1-R2)== |
| |PDB= 3boe |SIZE=350|CAPTION= <scene name='initialview01'>3boe</scene>, resolution 1.40Å
| | <StructureSection load='3boe' size='340' side='right'caption='[[3boe]], [[Resolution|resolution]] 1.40Å' scene=''> |
| |SITE= <scene name='pdbsite=AC1:Cd+Binding+Site+For+Residue+A+1001'>AC1</scene> and <scene name='pdbsite=AC2:Act+Binding+Site+For+Residue+A+1002'>AC2</scene>
| | == Structural highlights == |
| |LIGAND= <scene name='pdbligand=CD:CADMIUM+ION'>CD</scene> and <scene name='pdbligand=ACT:ACETATE ION'>ACT</scene>
| | <table><tr><td colspan='2'>[[3boe]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Conticribra_weissflogii Conticribra weissflogii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3BOE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3BOE FirstGlance]. <br> |
| |ACTIVITY=
| | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.4Å</td></tr> |
| |GENE= cdca1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=67004 Thalassiosira weissflogii])
| | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=CD:CADMIUM+ION'>CD</scene></td></tr> |
| }}
| | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3boe FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3boe OCA], [https://pdbe.org/3boe PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3boe RCSB], [https://www.ebi.ac.uk/pdbsum/3boe PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3boe ProSAT]</span></td></tr> |
| | </table> |
| | == Function == |
| | [https://www.uniprot.org/uniprot/Q50EL4_THAWE Q50EL4_THAWE] |
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| '''Carbonic anhydrase from marine diatom Thalassiosira weissflogii- cadmium bound domain 2 with acetate (CDCA1-R2)'''
| | ==See Also== |
| | | *[[Carbonic anhydrase 3D structures|Carbonic anhydrase 3D structures]] |
| | | __TOC__ |
| ==Overview== | | </StructureSection> |
| Carbonic anhydrase, a zinc enzyme found in organisms from all kingdoms, catalyses the reversible hydration of carbon dioxide and is used for inorganic carbon acquisition by phytoplankton. In the oceans, where zinc is nearly depleted, diatoms use cadmium as a catalytic metal atom in cadmium carbonic anhydrase (CDCA). Here we report the crystal structures of CDCA in four distinct forms: cadmium-bound, zinc-bound, metal-free and acetate-bound. Despite lack of sequence homology, CDCA is a structural mimic of a functional beta-carbonic anhydrase dimer, with striking similarity in the spatial organization of the active site residues. CDCA readily exchanges cadmium and zinc at its active site--an apparently unique adaptation to oceanic life that is explained by a stable opening of the metal coordinating site in the absence of metal. Given the central role of diatoms in exporting carbon to the deep sea, their use of cadmium in an enzyme critical for carbon acquisition establishes a remarkable link between the global cycles of cadmium and carbon. | | [[Category: Conticribra weissflogii]] |
| | | [[Category: Large Structures]] |
| ==About this Structure==
| | [[Category: Feng L]] |
| 3BOE is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Thalassiosira_weissflogii Thalassiosira weissflogii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3BOE OCA].
| | [[Category: Jeffrey PD]] |
| | | [[Category: Morel FMM]] |
| ==Reference==
| | [[Category: Shi Y]] |
| Structure and metal exchange in the cadmium carbonic anhydrase of marine diatoms., Xu Y, Feng L, Jeffrey PD, Shi Y, Morel FM, Nature. 2008 Mar 6;452(7183):56-61. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18322527 18322527]
| | [[Category: Xu Y]] |
| [[Category: Single protein]] | |
| [[Category: Thalassiosira weissflogii]] | |
| [[Category: Feng, L.]] | |
| [[Category: Jeffrey, P D.]] | |
| [[Category: Morel, F M.M.]] | |
| [[Category: Shi, Y.]] | |
| [[Category: Xu, Y.]] | |
| [[Category: ACT]]
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| [[Category: CD]]
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| [[Category: acetate ligand]]
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| [[Category: cadmium-bound]]
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| [[Category: carbonic anhydrase]]
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| [[Category: marine diatom]]
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| [[Category: oxidoreductase]]
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| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 19:00:18 2008''
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