3b4c: Difference between revisions

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-[[Image:3b4c.gif|left|200px]]<br /><applet load="3b4c" size="350" color="white" frame="true" align="right" spinBox="true"
-caption="3b4c, resolution 3.0&Aring;" />
-'''T. tengcongensis glmS ribozyme bound to glucosamine-6-phosphate and a substrate RNA with a 2'5'-phosphodiester linkage'''<br />
-==Overview==
+==T. tengcongensis glmS ribozyme bound to glucosamine-6-phosphate and a substrate RNA with a 2'5'-phosphodiester linkage==
-The glmS ribozyme is a catalytic riboswitch that is activated for endonucleolytic cleavage by the coenzyme glucosamine-6-phosphate. Using kinetic assays and X-ray crystallography, we identify an active-site mutation of a conserved guanine that abolishes catalysis without perturbing coenzyme binding. Our results provide evidence that coenzyme function requires a specific nucleobase to interact with the nucleophile of the cleavage reaction.
+<StructureSection load='3b4c' size='340' side='right'caption='[[3b4c]], [[Resolution|resolution]] 3.00&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[3b4c]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Caldanaerobacter_subterraneus_subsp._tengcongensis Caldanaerobacter subterraneus subsp. tengcongensis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3B4C OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3B4C FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3&#8491;</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=3AD:3-DEOXYADENOSINE'>3AD</scene>, <scene name='pdbligand=GLP:GLUCOSAMINE+6-PHOSPHATE'>GLP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3b4c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3b4c OCA], [https://pdbe.org/3b4c PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3b4c RCSB], [https://www.ebi.ac.uk/pdbsum/3b4c PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3b4c ProSAT]</span></td></tr>
+</table>
-==About this Structure==
+==See Also==
-B4C is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Thermoanaerobacter_tengcongensis Thermoanaerobacter tengcongensis] with <scene name='pdbligand=GLP:'>GLP</scene>, <scene name='pdbligand=MG:'>MG</scene> and <scene name='pdbligand=3AD:'>3AD</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3B4C OCA].
+*[[Ribozyme 3D structures|Ribozyme 3D structures]]
+__TOC__
-==Reference==
+</StructureSection>
-Essential role of an active-site guanine in glmS ribozyme catalysis., Klein DJ, Been MD, Ferre-D'Amare AR, J Am Chem Soc. 2007 Dec 5;129(48):14858-9. Epub 2007 Nov 9. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17990888 17990888]
+[[Category: Caldanaerobacter subterraneus subsp. tengcongensis]]
-[[Category: Protein complex]]
+[[Category: Large Structures]]
-[[Category: Thermoanaerobacter tengcongensis]]
+[[Category: Ferre-D'Amare AR]]
-[[Category: Amare, A R.Ferre-D.]]
+[[Category: Klein DJ]]
-[[Category: Klein, D J.]]
-[[Category: 3AD]]
-[[Category: GLP]]
-[[Category: MG]]
-[[Category: glucosamine-6-phosphate]]
-[[Category: riboswitch]]
-[[Category: ribozyme]]
-[[Category: rna]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 19:03:16 2008''