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[[Image:3b4b.gif|left|200px]]


{{Structure
==T. tengcongensis glmS ribozyme with G40A mutation, bound to glucosamine-6-phosphate and a substrate RNA with a 2'5'-phosphodiester linkage==
|PDB= 3b4b |SIZE=350|CAPTION= <scene name='initialview01'>3b4b</scene>, resolution 2.7&Aring;
<StructureSection load='3b4b' size='340' side='right'caption='[[3b4b]], [[Resolution|resolution]] 2.70&Aring;' scene=''>
|SITE=  
== Structural highlights ==
|LIGAND= <scene name='pdbligand=GLP:GLUCOSAMINE+6-PHOSPHATE'>GLP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene> and <scene name='pdbligand=3AD:3&#39;-DEOXYADENOSINE'>3AD</scene>
<table><tr><td colspan='2'>[[3b4b]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Caldanaerobacter_subterraneus_subsp._tengcongensis Caldanaerobacter subterraneus subsp. tengcongensis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3B4B OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3B4B FirstGlance]. <br>
|ACTIVITY=  
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.7&#8491;</td></tr>
|GENE=  
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=3AD:3-DEOXYADENOSINE'>3AD</scene>, <scene name='pdbligand=GLP:GLUCOSAMINE+6-PHOSPHATE'>GLP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
}}
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3b4b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3b4b OCA], [https://pdbe.org/3b4b PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3b4b RCSB], [https://www.ebi.ac.uk/pdbsum/3b4b PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3b4b ProSAT]</span></td></tr>
</table>


'''T. tengcongensis glmS ribozyme with G40A mutation, bound to glucosamine-6-phosphate and a substrate RNA with a 2'5'-phosphodiester linkage'''
==See Also==
 
*[[Ribozyme 3D structures|Ribozyme 3D structures]]
 
__TOC__
==Overview==
</StructureSection>
The glmS ribozyme is a catalytic riboswitch that is activated for endonucleolytic cleavage by the coenzyme glucosamine-6-phosphate. Using kinetic assays and X-ray crystallography, we identify an active-site mutation of a conserved guanine that abolishes catalysis without perturbing coenzyme binding. Our results provide evidence that coenzyme function requires a specific nucleobase to interact with the nucleophile of the cleavage reaction.
[[Category: Caldanaerobacter subterraneus subsp. tengcongensis]]
 
[[Category: Large Structures]]
==About this Structure==
[[Category: Ferre-D'Amare AR]]
3B4B is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Thermoanaerobacter_tengcongensis Thermoanaerobacter tengcongensis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3B4B OCA].
[[Category: Klein DJ]]
 
==Reference==
Essential role of an active-site guanine in glmS ribozyme catalysis., Klein DJ, Been MD, Ferre-D'Amare AR, J Am Chem Soc. 2007 Dec 5;129(48):14858-9. Epub 2007 Nov 9. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17990888 17990888]
[[Category: Protein complex]]
[[Category: Thermoanaerobacter tengcongensis]]
[[Category: Amare, A R.Ferre-D.]]
[[Category: Klein, D J.]]
[[Category: 3AD]]
[[Category: GLP]]
[[Category: MG]]
[[Category: glucosamine-6-phosphate]]
[[Category: riboswitch]]
[[Category: ribozyme]]
[[Category: rna]]
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 23 15:58:16 2008''

Latest revision as of 12:58, 14 February 2024

T. tengcongensis glmS ribozyme with G40A mutation, bound to glucosamine-6-phosphate and a substrate RNA with a 2'5'-phosphodiester linkageT. tengcongensis glmS ribozyme with G40A mutation, bound to glucosamine-6-phosphate and a substrate RNA with a 2'5'-phosphodiester linkage

Structural highlights

3b4b is a 2 chain structure with sequence from Caldanaerobacter subterraneus subsp. tengcongensis. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 2.7Å
Ligands:, ,
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

See Also

3b4b, resolution 2.70Å

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