2hqg: Difference between revisions

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
-[[Image:2hqg.gif|left|200px]]<br /><applet load="2hqg" size="450" color="white" frame="true" align="right" spinBox="true"
-caption="2hqg, resolution 3.38&Aring;" />
-'''Conformation of the AcrB Multidrug Efflux Pump in Mutants of the Putative Proton Relay Pathway'''<br />
-==Overview==
+==Conformation of the AcrB Multidrug Efflux Pump in Mutants of the Putative Proton Relay Pathway==
-We previously reported the X-ray structures of wild-type Escherichia coli, AcrB, a proton motive force-dependent multidrug efflux pump, and its N109A, mutant. These structures presumably reflect the resting state of AcrB, which can bind drugs. After ligand binding, a proton may bind to an acidic, residue(s) in the transmembrane domain, i.e., Asp407 or Asp408, within the, putative network of electrostatically interacting residues, which also, include Lys940 and Thr978, and this may initiate a series of, conformational changes that result in drug expulsion. Herein we report the, X-ray structures of four AcrB mutants, the D407A, D408A, K940A, and T978A, mutants, in which the structure of this tight electrostatic network is, expected to become disrupted. These mutant proteins revealed remarkably, similar conformations, which show striking differences from the previously, known conformations of the wild-type protein. For example, the loop, containing Phe386 and Phe388, which play a major role in the initial, binding of substrates in the central cavity, becomes prominently extended, into the center of the cavity, such that binding of large substrate, molecules may become difficult. We believe that this new conformation may, mimic, at least partially, one of the transient conformations of the, transporter during the transport cycle.
+<StructureSection load='2hqg' size='340' side='right'caption='[[2hqg]], [[Resolution|resolution]] 3.38&Aring;' scene=''>
+== Structural highlights ==
-==About this Structure==
+<table><tr><td colspan='2'>[[2hqg]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HQG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2HQG FirstGlance]. <br>
-HQG is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2HQG OCA].
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.38&#8491;</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2hqg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2hqg OCA], [https://pdbe.org/2hqg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2hqg RCSB], [https://www.ebi.ac.uk/pdbsum/2hqg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2hqg ProSAT]</span></td></tr>
-==Reference==
+</table>
-Conformation of the AcrB multidrug efflux pump in mutants of the putative proton relay pathway., Su CC, Li M, Gu R, Takatsuka Y, McDermott G, Nikaido H, Yu EW, J Bacteriol. 2006 Oct;188(20):7290-6. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17015668 17015668]
+== Function ==
-[[Category: Escherichia coli]]
+[https://www.uniprot.org/uniprot/ACRB_ECOLI ACRB_ECOLI] AcrAB is a drug efflux protein with a broad substrate specificity.<ref>PMID:16915237</ref> <ref>PMID:16946072</ref> <ref>PMID:17194213</ref>
-[[Category: Single protein]]
+== Evolutionary Conservation ==
-[[Category: Gu, R.]]
+[[Image:Consurf_key_small.gif|200px|right]]
-[[Category: Li, M.]]
+Check<jmol>
-[[Category: McDermott, G.]]
+  <jmolCheckbox>
-[[Category: Nikaido, H.]]
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/hq/2hqg_consurf.spt"</scriptWhenChecked>
-[[Category: Su, C.C.]]
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
-[[Category: Takatsuka, Y.]]
+    <text>to colour the structure by Evolutionary Conservation</text>
-[[Category: Yu, E.W.]]
+  </jmolCheckbox>
-[[Category: membrane protein]]
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2hqg ConSurf].
-[[Category: multidrug efflux pump]]
+<div style="clear:both"></div>
+== References ==
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 11:54:50 2007''
+<references/>
+__TOC__
+</StructureSection>
+[[Category: Escherichia coli K-12]]
+[[Category: Large Structures]]
+[[Category: Gu R]]
+[[Category: Li M]]
+[[Category: McDermott G]]
+[[Category: Nikaido H]]
+[[Category: Su C-C]]
+[[Category: Takatsuka Y]]
+[[Category: Yu EW]]