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| [[Image:2hoo.gif|left|200px]]<br /><applet load="2hoo" size="350" color="white" frame="true" align="right" spinBox="true"
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| caption="2hoo, resolution 3.00Å" />
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| '''Crystal structure of an E. coli thi-box riboswitch bound to benfotiamine'''<br />
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| ==Overview== | | ==Crystal structure of an E. coli thi-box riboswitch bound to benfotiamine== |
| Riboswitches are noncoding mRNA elements that bind small-molecule metabolites with high affinity and specificity, and they regulate the expression of associated genes. The thi-box riboswitch can exhibit a 1000-fold higher affinity for thiamine pyrophosphate over closely related noncognate compounds such as thiamine monophosphate. To understand the chemical basis of thi-box pyrophosphate specificity, we have determined crystal structures of an E. coli thi-box bound to thiamine pyrophosphate, thiamine monophosphate, and the structural analogs benfotiamine and pyrithiamine. When bound to monophosphorylated compounds, the RNA elements that recognize the thiamine and phosphate moieties of the ligand move closer together. This allows the riboswitch to recognize the monophosphate in a manner similar to how it recognizes the beta-phosphate of thiamine pyrophosphate. In the pyrithiamine complex, the pyrophosphate binding site is largely unstructured. These results show how the riboswitch can bind to various metabolites, and why the thi-box preferentially binds thiamine pyrophosphate.
| | <StructureSection load='2hoo' size='340' side='right'caption='[[2hoo]], [[Resolution|resolution]] 3.00Å' scene=''> |
| | == Structural highlights == |
| | <table><tr><td colspan='2'>[[2hoo]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HOO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2HOO FirstGlance]. <br> |
| | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3Å</td></tr> |
| | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BFT:S-BENZOYLTHIAMINE+O-MONOPHOSPHATE'>BFT</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr> |
| | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2hoo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2hoo OCA], [https://pdbe.org/2hoo PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2hoo RCSB], [https://www.ebi.ac.uk/pdbsum/2hoo PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2hoo ProSAT]</span></td></tr> |
| | </table> |
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| ==About this Structure== | | ==See Also== |
| 2HOO is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ] with <scene name='pdbligand=CA:'>CA</scene> and <scene name='pdbligand=BFT:'>BFT</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HOO OCA].
| | *[[Riboswitch 3D structures|Riboswitch 3D structures]] |
| | | *[[Ribozyme 3D structures|Ribozyme 3D structures]] |
| ==Reference==
| | __TOC__ |
| Crystal structures of the thi-box riboswitch bound to thiamine pyrophosphate analogs reveal adaptive RNA-small molecule recognition., Edwards TE, Ferre-D'Amare AR, Structure. 2006 Sep;14(9):1459-68. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16962976 16962976]
| | </StructureSection> |
| [[Category: Protein complex]] | | [[Category: Escherichia coli]] |
| [[Category: Amare, A R.Ferre-D.]] | | [[Category: Large Structures]] |
| [[Category: Edwards, T E.]] | | [[Category: Edwards TE]] |
| [[Category: BFT]] | | [[Category: Ferre-D'Amare AR]] |
| [[Category: CA]]
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| [[Category: rna; riboswitch; benfotiamine]]
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| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:43:59 2008''
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