2hh7: Difference between revisions

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New page: left|200px<br /><applet load="2hh7" size="450" color="white" frame="true" align="right" spinBox="true" caption="2hh7, resolution 2.55Å" /> '''Crystal Structure of...
 
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[[Image:2hh7.gif|left|200px]]<br /><applet load="2hh7" size="450" color="white" frame="true" align="right" spinBox="true"
caption="2hh7, resolution 2.55&Aring;" />
'''Crystal Structure of Cu(I) bound CsoR from Mycobacterium tuberculosis.'''<br />


==Overview==
==Crystal Structure of Cu(I) bound CsoR from Mycobacterium tuberculosis.==
Copper is an essential element that becomes highly cytotoxic when, concentrations exceed the capacity of cells to sequester the ion. Here, we, identify a new copper-specific repressor (CsoR) of a copper-sensitive, operon (cso) in Mycobacterium tuberculosis (Mtb) that is representative of, a large, previously uncharacterized family of proteins (DUF156)., Electronic and X-ray absorption spectroscopies reveal that CsoR binds a, single-monomer mole equivalent of Cu(I) to form a trigonally coordinated, (S(2)N) Cu(I) complex. The 2.6-A crystal structure of copper-loaded CsoR, shows a homodimeric antiparallel four-helix bundle architecture that, represents a novel DNA-binding fold. The Cu(I) is coordinated by Cys36, Cys65' and His61' in a subunit bridging site. Cu(I) binding negatively, regulates the binding of CsoR to a DNA fragment encompassing the, operator-promoter region of the Mtb cso operon; this results in, derepression of the operon in Mtb and the heterologous host Mycobacterium, smegmatis. Substitution of Cys36 or His61 with alanine abolishes Cu(I)-, and CsoR-dependent regulation in vivo and in vitro. Potential roles of, CsoR in Mtb pathogenesis are discussed.
<StructureSection load='2hh7' size='340' side='right'caption='[[2hh7]], [[Resolution|resolution]] 2.55&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[2hh7]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HH7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2HH7 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.55&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CU1:COPPER+(I)+ION'>CU1</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2hh7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2hh7 OCA], [https://pdbe.org/2hh7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2hh7 RCSB], [https://www.ebi.ac.uk/pdbsum/2hh7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2hh7 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/CSOR_MYCTU CSOR_MYCTU] Copper-sensitive repressor that has a key role in copper homeostasis. It is part of the cso operon involved in the cellular response to increasing concentrations of copper inside the bacterium, which can be highly toxic. In the presence of copper, CsoR fully dissociates from the promoter in the cso operon, leading to the transcription of its genes. Binds to a GC-rich pseudopallindromic sequence, 5'-GTAGCCCACCCCCAGTGGGGTGGGA-3', in the cso promoter region.<ref>PMID:17143269</ref>
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/hh/2hh7_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2hh7 ConSurf].
<div style="clear:both"></div>


==About this Structure==
==See Also==
2HH7 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis] with CU1 as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2HH7 OCA].
*[[Copper homeostasis protein|Copper homeostasis protein]]
 
== References ==
==Reference==
<references/>
CsoR is a novel Mycobacterium tuberculosis copper-sensing transcriptional regulator., Liu T, Ramesh A, Ma Z, Ward SK, Zhang L, George GN, Talaat AM, Sacchettini JC, Giedroc DP, Nat Chem Biol. 2007 Jan;3(1):60-8. Epub 2006 Dec 3. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17143269 17143269]
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Mycobacterium tuberculosis]]
[[Category: Mycobacterium tuberculosis]]
[[Category: Single protein]]
[[Category: Ramesh A]]
[[Category: Ramesh, A.]]
[[Category: Sacchettini JC]]
[[Category: Sacchettini,J.C.]]
[[Category: CU1]]
[[Category: 4-helix bundle]]
 
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 11:46:09 2007''

Latest revision as of 12:32, 14 February 2024

Crystal Structure of Cu(I) bound CsoR from Mycobacterium tuberculosis.Crystal Structure of Cu(I) bound CsoR from Mycobacterium tuberculosis.

Structural highlights

2hh7 is a 1 chain structure with sequence from Mycobacterium tuberculosis. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 2.55Å
Ligands:
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

CSOR_MYCTU Copper-sensitive repressor that has a key role in copper homeostasis. It is part of the cso operon involved in the cellular response to increasing concentrations of copper inside the bacterium, which can be highly toxic. In the presence of copper, CsoR fully dissociates from the promoter in the cso operon, leading to the transcription of its genes. Binds to a GC-rich pseudopallindromic sequence, 5'-GTAGCCCACCCCCAGTGGGGTGGGA-3', in the cso promoter region.[1]

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

See Also

References

  1. ↑ Liu T, Ramesh A, Ma Z, Ward SK, Zhang L, George GN, Talaat AM, Sacchettini JC, Giedroc DP. CsoR is a novel Mycobacterium tuberculosis copper-sensing transcriptional regulator. Nat Chem Biol. 2007 Jan;3(1):60-8. Epub 2006 Dec 3. PMID:17143269 doi:http://dx.doi.org/10.1038/nchembio844

2hh7, resolution 2.55Å

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