2hc8: Difference between revisions

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New page: left|200px<br /><applet load="2hc8" size="350" color="white" frame="true" align="right" spinBox="true" caption="2hc8, resolution 1.650Å" /> '''Structure of the A....
 
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[[Image:2hc8.jpg|left|200px]]<br /><applet load="2hc8" size="350" color="white" frame="true" align="right" spinBox="true"
caption="2hc8, resolution 1.650&Aring;" />
'''Structure of the A. fulgidus CopA A-domain'''<br />


==Overview==
==Structure of the A. fulgidus CopA A-domain==
Copper homeostasis is maintained in part by membrane-bound P(1B)-type, ATPases that are found in all organisms and drive the transport of this, essential, yet toxic, metal ion across cellular membranes. CopA from, Archaeoglobus fulgidus is a hyperthermophilic member of this ATPase, subfamily and is homologous to the human Wilson and Menkes disease, ATPases. To gain insight into Cu(+)-ATPase function, the structure of the, CopA actuator domain (A-domain) was determined to 1.65 A resolution. The, CopA A-domain functions to couple ATP hydrolysis in the ATP binding domain, (ATPBD) with structural rearrangements of critical transmembrane segments., Its fold is quite similar to that of the sarcoplasmic reticulum, Ca(2+)-ATPase (SERCA1) A-domain, with the exception of an external loop, region. On the basis of sequence and structural comparisons, specific, residues that probably interact with the CopA ATPBD have been identified., Comparisons to the Wilson and Menkes disease A-domains reveal the presence, of an additional loop that may be associated with regulatory functions in, eukaryotic Cu(+)-ATPases. Finally, several mutations in the Wilson and, Menkes disease ATPases occur in the A-domain, and their likely effects on, function can be inferred from the CopA A-domain structure.
<StructureSection load='2hc8' size='340' side='right'caption='[[2hc8]], [[Resolution|resolution]] 1.65&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[2hc8]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Archaeoglobus_fulgidus Archaeoglobus fulgidus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HC8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2HC8 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.65&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2hc8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2hc8 OCA], [https://pdbe.org/2hc8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2hc8 RCSB], [https://www.ebi.ac.uk/pdbsum/2hc8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2hc8 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/COPA_ARCFU COPA_ARCFU] Probably involved in copper and silver export.<ref>PMID:11756450</ref>
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/hc/2hc8_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2hc8 ConSurf].
<div style="clear:both"></div>


==About this Structure==
==See Also==
2HC8 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Archaeoglobus_fulgidus Archaeoglobus fulgidus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HC8 OCA].
*[[ATPase 3D structures|ATPase 3D structures]]
 
== References ==
==Reference==
<references/>
Structure of the actuator domain from the Archaeoglobus fulgidus Cu(+)-ATPase., Sazinsky MH, Agarwal S, Arguello JM, Rosenzweig AC, Biochemistry. 2006 Aug 22;45(33):9949-55. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16906753 16906753]
__TOC__
</StructureSection>
[[Category: Archaeoglobus fulgidus]]
[[Category: Archaeoglobus fulgidus]]
[[Category: Single protein]]
[[Category: Large Structures]]
[[Category: Agawal, S.]]
[[Category: Agawal S]]
[[Category: Arguello, J.M.]]
[[Category: Arguello JM]]
[[Category: Rosenzweig, A.C.]]
[[Category: Rosenzweig AC]]
[[Category: Sazinsky, M.H.]]
[[Category: Sazinsky MH]]
[[Category: actuator]]
[[Category: atpase]]
[[Category: copa]]
[[Category: copb]]
[[Category: copper]]
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jan 29 20:18:12 2008''

Latest revision as of 12:31, 14 February 2024

Structure of the A. fulgidus CopA A-domainStructure of the A. fulgidus CopA A-domain

Structural highlights

2hc8 is a 1 chain structure with sequence from Archaeoglobus fulgidus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 1.65Å
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

COPA_ARCFU Probably involved in copper and silver export.[1]

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

See Also

References

  1. ↑ Mandal AK, Cheung WD, Arguello JM. Characterization of a thermophilic P-type Ag+/Cu+-ATPase from the extremophile Archaeoglobus fulgidus. J Biol Chem. 2002 Mar 1;277(9):7201-8. Epub 2001 Dec 26. PMID:11756450 doi:10.1074/jbc.M109964200

2hc8, resolution 1.65Å

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