2h0x: Difference between revisions

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-[[Image:2h0x.gif|left|200px]]
- {{Structure
+==Pre-cleavage state of the Thermoanaerobacter tengcongensis glmS ribozyme==
-|PDB= 2h0x |SIZE=350|CAPTION= <scene name='initialview01'>2h0x</scene>, resolution 2.30&Aring;
+<StructureSection load='2h0x' size='340' side='right'caption='[[2h0x]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
-|SITE=
+== Structural highlights ==
-|LIGAND= <scene name='pdbligand=2AD:2&#39;-AMINO-2&#39;-DEOXYADENOSINE'>2AD</scene>, <scene name='pdbligand=A:ADENOSINE-5&#39;-MONOPHOSPHATE'>A</scene>, <scene name='pdbligand=A23:ADENOSINE-5&#39;-PHOSPHATE-2&#39;,3&#39;-CYCLIC+PHOSPHATE'>A23</scene>, <scene name='pdbligand=C:CYTIDINE-5&#39;-MONOPHOSPHATE'>C</scene>, <scene name='pdbligand=G:GUANOSINE-5&#39;-MONOPHOSPHATE'>G</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=U:URIDINE-5&#39;-MONOPHOSPHATE'>U</scene>
+<table><tr><td colspan='2'>[[2h0x]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Caldanaerobacter_subterraneus_subsp._tengcongensis Caldanaerobacter subterraneus subsp. tengcongensis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2H0X OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2H0X FirstGlance]. <br>
-|ACTIVITY=
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3&#8491;</td></tr>
-|GENE=
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=2AD:2-AMINO-2-DEOXYADENOSINE'>2AD</scene>, <scene name='pdbligand=A23:ADENOSINE-5-PHOSPHATE-2,3-CYCLIC+PHOSPHATE'>A23</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
-|DOMAIN=
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2h0x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2h0x OCA], [https://pdbe.org/2h0x PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2h0x RCSB], [https://www.ebi.ac.uk/pdbsum/2h0x PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2h0x ProSAT]</span></td></tr>
-|RELATEDENTRY=[[2gcs|2GCS]], [[2gcv|2GCV]], [[2h0s|2H0S]], [[2h0w|2H0W]], [[2h0z|2H0Z]]
+</table>
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2h0x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2h0x OCA], [http://www.ebi.ac.uk/pdbsum/2h0x PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2h0x RCSB]</span>
-}}
-'''Pre-cleavage state of the Thermoanaerobacter tengcongensis glmS ribozyme'''
+==See Also==
+*[[Ribozyme 3D structures|Ribozyme 3D structures]]
+__TOC__
-==Overview==
+</StructureSection>
-The glmS ribozyme is the only natural catalytic RNA known to require a small-molecule activator for catalysis. This catalytic RNA functions as a riboswitch, with activator-dependent RNA cleavage regulating glmS messenger RNA expression. We report crystal structures of the glmS ribozyme in precleavage states that are unliganded or bound to the competitive inhibitor glucose-6-phosphate and in the postcleavage state. All structures superimpose closely, revealing a remarkably rigid RNA that contains a preformed active and coenzyme-binding site. Unlike other riboswitches, the glmS ribozyme binds its activator in an open, solvent-accessible pocket. Our structures suggest that the amine group of the glmS ribozyme-bound coenzyme performs general acid-base and electrostatic catalysis.
+[[Category: Caldanaerobacter subterraneus subsp. tengcongensis]]
+[[Category: Large Structures]]
-==About this Structure==
+[[Category: Ferre-D'Amare AR]]
-H0X is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2H0X OCA].
+[[Category: Klein DJ]]
-==Reference==
-Structural basis of glmS ribozyme activation by glucosamine-6-phosphate., Klein DJ, Ferre-D'Amare AR, Science. 2006 Sep 22;313(5794):1752-6. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16990543 16990543]
-[[Category: Single protein]]
-[[Category: Amare, A R.Ferre-D.]]
-[[Category: Klein, D J.]]
-[[Category: helix]]
-[[Category: pseudoknot]]
-[[Category: ribozyme]]
-[[Category: rna]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 03:23:53 2008''