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[[Image:2gt1.jpg|left|200px]]<br /><applet load="2gt1" size="350" color="white" frame="true" align="right" spinBox="true"
caption="2gt1, resolution 1.900&Aring;" />
'''E. coli heptosyltransferase WaaC.'''<br />


==Overview==
==E. coli heptosyltransferase WaaC.==
Lipopolysaccharides constitute the outer leaflet of the outer membrane of Gram-negative bacteria and are therefore essential for cell growth and viability. The heptosyltransferase WaaC is a glycosyltransferase (GT) involved in the synthesis of the inner core region of LPS. It catalyzes the addition of the first L-glycero-D-manno-heptose (heptose) molecule to one 3-deoxy-D-manno-oct-2-ulosonic acid (Kdo) residue of the Kdo2-lipid A molecule. Heptose is an essential component of the LPS core domain; its absence results in a truncated lipopolysaccharide associated with the deep-rough phenotype causing a greater susceptibility to antibiotic and an attenuated virulence for pathogenic Gram-negative bacteria. Thus, WaaC represents a promising target in antibacterial drug design. Here, we report the structure of WaaC from the Escherichia coli pathogenic strain RS218 alone at 1.9 A resolution, and in complex with either ADP or the non-cleavable analog ADP-2-deoxy-2-fluoro-heptose of the sugar donor at 2.4 A resolution. WaaC adopts the GT-B fold in two domains, characteristic of one glycosyltransferase structural superfamily. The comparison of the three different structures shows that WaaC does not undergo a domain rotation, characteristic of the GT-B family, upon substrate binding, but allows the substrate analog and the reaction product to adopt remarkably distinct conformations inside the active site. In addition, both binary complexes offer a close view of the donor subsite and, together with results from site-directed mutagenesis studies, provide evidence for a model of the catalytic mechanism.
<StructureSection load='2gt1' size='340' side='right'caption='[[2gt1]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
 
== Structural highlights ==
==About this Structure==
<table><tr><td colspan='2'>[[2gt1]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_UTI89 Escherichia coli UTI89]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GT1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2GT1 FirstGlance]. <br>
2GT1 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli_uti89 Escherichia coli uti89]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GT1 OCA].  
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9&#8491;</td></tr>
 
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2gt1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2gt1 OCA], [https://pdbe.org/2gt1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2gt1 RCSB], [https://www.ebi.ac.uk/pdbsum/2gt1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2gt1 ProSAT]</span></td></tr>
==Reference==
</table>
Structure of the Escherichia coli heptosyltransferase WaaC: binary complexes with ADP and ADP-2-deoxy-2-fluoro heptose., Grizot S, Salem M, Vongsouthi V, Durand L, Moreau F, Dohi H, Vincent S, Escaich S, Ducruix A, J Mol Biol. 2006 Oct 20;363(2):383-94. Epub 2006 Jul 29. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16963083 16963083]
== Function ==
[[Category: Escherichia coli uti89]]
[https://www.uniprot.org/uniprot/Q1R4X0_ECOUT Q1R4X0_ECOUT]
[[Category: Single protein]]
== Evolutionary Conservation ==
[[Category: Dohi, H.]]
[[Image:Consurf_key_small.gif|200px|right]]
[[Category: Ducruix, A.]]
Check<jmol>
[[Category: Durand, L.]]
  <jmolCheckbox>
[[Category: Escaich, S.]]
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/gt/2gt1_consurf.spt"</scriptWhenChecked>
[[Category: Grizot, S.]]
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
[[Category: Moreau, F.]]
    <text>to colour the structure by Evolutionary Conservation</text>
[[Category: Salem, M.]]
  </jmolCheckbox>
[[Category: Vincent, S.]]
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2gt1 ConSurf].
[[Category: Vongsouthi, V.]]
<div style="clear:both"></div>
[[Category: gt-b fold]]
__TOC__
 
</StructureSection>
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:35:04 2008''
[[Category: Escherichia coli UTI89]]
[[Category: Large Structures]]
[[Category: Dohi H]]
[[Category: Ducruix A]]
[[Category: Durand L]]
[[Category: Escaich S]]
[[Category: Grizot S]]
[[Category: Moreau F]]
[[Category: Salem M]]
[[Category: Vincent S]]
[[Category: Vongsouthi V]]

Latest revision as of 12:29, 14 February 2024

E. coli heptosyltransferase WaaC.E. coli heptosyltransferase WaaC.

Structural highlights

2gt1 is a 2 chain structure with sequence from Escherichia coli UTI89. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 1.9Å
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

Q1R4X0_ECOUT

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

2gt1, resolution 1.90Å

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA
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