2gt1: Difference between revisions

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OCA

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-[[Image:2gt1.jpg|left|200px]]<br /><applet load="2gt1" size="350" color="white" frame="true" align="right" spinBox="true"
-caption="2gt1, resolution 1.900&Aring;" />
-'''E. coli heptosyltransferase WaaC.'''<br />
-==Overview==
+==E. coli heptosyltransferase WaaC.==
-Lipopolysaccharides constitute the outer leaflet of the outer membrane of, Gram-negative bacteria and are therefore essential for cell growth and, viability. The heptosyltransferase WaaC is a glycosyltransferase (GT), involved in the synthesis of the inner core region of LPS. It catalyzes, the addition of the first L-glycero-D-manno-heptose (heptose) molecule to, one 3-deoxy-D-manno-oct-2-ulosonic acid (Kdo) residue of the Kdo2-lipid A, molecule. Heptose is an essential component of the LPS core domain; its, absence results in a truncated lipopolysaccharide associated with the, deep-rough phenotype causing a greater susceptibility to antibiotic and an, attenuated virulence for pathogenic Gram-negative bacteria. Thus, WaaC, represents a promising target in antibacterial drug design. Here, we, report the structure of WaaC from the Escherichia coli pathogenic strain, RS218 alone at 1.9 A resolution, and in complex with either ADP or the, non-cleavable analog ADP-2-deoxy-2-fluoro-heptose of the sugar donor at, 2.4 A resolution. WaaC adopts the GT-B fold in two domains, characteristic, of one glycosyltransferase structural superfamily. The comparison of the, three different structures shows that WaaC does not undergo a domain, rotation, characteristic of the GT-B family, upon substrate binding, but, allows the substrate analog and the reaction product to adopt remarkably, distinct conformations inside the active site. In addition, both binary, complexes offer a close view of the donor subsite and, together with, results from site-directed mutagenesis studies, provide evidence for a, model of the catalytic mechanism.
+<StructureSection load='2gt1' size='340' side='right'caption='[[2gt1]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
+== Structural highlights ==
-==About this Structure==
+<table><tr><td colspan='2'>[[2gt1]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_UTI89 Escherichia coli UTI89]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GT1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2GT1 FirstGlance]. <br>
-GT1 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli_uti89 Escherichia coli uti89]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GT1 OCA].
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9&#8491;</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2gt1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2gt1 OCA], [https://pdbe.org/2gt1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2gt1 RCSB], [https://www.ebi.ac.uk/pdbsum/2gt1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2gt1 ProSAT]</span></td></tr>
-==Reference==
+</table>
-Structure of the Escherichia coli heptosyltransferase WaaC: binary complexes with ADP and ADP-2-deoxy-2-fluoro heptose., Grizot S, Salem M, Vongsouthi V, Durand L, Moreau F, Dohi H, Vincent S, Escaich S, Ducruix A, J Mol Biol. 2006 Oct 20;363(2):383-94. Epub 2006 Jul 29. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16963083 16963083]
+== Function ==
-[[Category: Escherichia coli uti89]]
+[https://www.uniprot.org/uniprot/Q1R4X0_ECOUT Q1R4X0_ECOUT]
-[[Category: Single protein]]
+== Evolutionary Conservation ==
-[[Category: Dohi, H.]]
+[[Image:Consurf_key_small.gif|200px|right]]
-[[Category: Ducruix, A.]]
+Check<jmol>
-[[Category: Durand, L.]]
+  <jmolCheckbox>
-[[Category: Escaich, S.]]
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/gt/2gt1_consurf.spt"</scriptWhenChecked>
-[[Category: Grizot, S.]]
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
-[[Category: Moreau, F.]]
+    <text>to colour the structure by Evolutionary Conservation</text>
-[[Category: Salem, M.]]
+  </jmolCheckbox>
-[[Category: Vincent, S.]]
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2gt1 ConSurf].
-[[Category: Vongsouthi, V.]]
+<div style="clear:both"></div>
-[[Category: gt-b fold]]
+__TOC__
+</StructureSection>
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jan 23 15:26:48 2008''
+[[Category: Escherichia coli UTI89]]
+[[Category: Large Structures]]
+[[Category: Dohi H]]
+[[Category: Ducruix A]]
+[[Category: Durand L]]
+[[Category: Escaich S]]
+[[Category: Grizot S]]
+[[Category: Moreau F]]
+[[Category: Salem M]]
+[[Category: Vincent S]]
+[[Category: Vongsouthi V]]