2gr8: Difference between revisions

From Proteopedia
Jump to navigation Jump to search
← Older edit
No edit summary
No edit summary
 
(12 intermediate revisions by the same user not shown)
Line 1: Line 1:
[[Image:2gr8.gif|left|200px]]


{{Structure
==Hia 1022-1098==
|PDB= 2gr8 |SIZE=350|CAPTION= <scene name='initialview01'>2gr8</scene>, resolution 2.00&Aring;
<StructureSection load='2gr8' size='340' side='right'caption='[[2gr8]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
|SITE=  
== Structural highlights ==
|LIGAND=  
<table><tr><td colspan='2'>[[2gr8]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Haemophilus_influenzae Haemophilus influenzae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GR8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2GR8 FirstGlance]. <br>
|ACTIVITY=  
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
|GENE=  
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2gr8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2gr8 OCA], [https://pdbe.org/2gr8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2gr8 RCSB], [https://www.ebi.ac.uk/pdbsum/2gr8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2gr8 ProSAT]</span></td></tr>
|DOMAIN=
</table>
|RELATEDENTRY=[[2gr7|2GR7]]
== Function ==
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2gr8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2gr8 OCA], [http://www.ebi.ac.uk/pdbsum/2gr8 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2gr8 RCSB]</span>
[https://www.uniprot.org/uniprot/Q48152_HAEIF Q48152_HAEIF]
}}
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/gr/2gr8_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2gr8 ConSurf].
<div style="clear:both"></div>


'''Hia 1022-1098'''
==See Also==
 
*[[Adhesin 3D structures|Adhesin 3D structures]]
 
__TOC__
==Overview==
</StructureSection>
Autotransporter proteins are defined by the ability to drive their own secretion across the bacterial outer membrane. The Hia autotransporter of Haemophilus influenzae belongs to the trimeric autotransporter subfamily and mediates bacterial adhesion to the respiratory epithelium. In this report, we present the crystal structure of the C-terminal end of Hia, corresponding to the entire Hia translocator domain and part of the passenger domain (residues 992-1098). This domain forms a beta-barrel with 12 transmembrane beta-strands, including four strands from each subunit. The beta-barrel has a central channel of 1.8 nm in diameter that is traversed by three N-terminal alpha-helices, one from each subunit. Mutagenesis studies demonstrate that the transmembrane portion of the three alpha-helices and the loop region between the alpha-helices and the neighboring beta-strands are essential for stability of the trimeric structure of the translocator domain, and that trimerization of the translocator domain is a prerequisite for translocator activity. Overall, this study provides important insights into the mechanism of translocation in trimeric autotransporters.
 
==About this Structure==
2GR8 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Haemophilus_influenzae Haemophilus influenzae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GR8 OCA].
 
==Reference==
Structure of the outer membrane translocator domain of the Haemophilus influenzae Hia trimeric autotransporter., Meng G, Surana NK, St Geme JW 3rd, Waksman G, EMBO J. 2006 Jun 7;25(11):2297-304. Epub 2006 May 11. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16688217 16688217]
[[Category: Haemophilus influenzae]]
[[Category: Haemophilus influenzae]]
[[Category: Single protein]]
[[Category: Large Structures]]
[[Category: Meng, G.]]
[[Category: Meng G]]
[[Category: Waksman, G.]]
[[Category: Waksman G]]
[[Category: adhesion]]
[[Category: crystal structure]]
[[Category: microbial pathogenesis]]
[[Category: protein secretion]]
[[Category: trimeric autotransporter]]
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 03:20:22 2008''

Latest revision as of 12:29, 14 February 2024

Hia 1022-1098Hia 1022-1098

Structural highlights

2gr8 is a 6 chain structure with sequence from Haemophilus influenzae. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 2Å
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

Q48152_HAEIF

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

See Also

2gr8, resolution 2.00Å

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA
Retrieved from "https://proteopedia.openfox.io/wiki/index.php?title=2gr8&oldid=4058795"