2gmh: Difference between revisions

From Proteopedia
Jump to navigation Jump to search
New page: left|200px<br /><applet load="2gmh" size="450" color="white" frame="true" align="right" spinBox="true" caption="2gmh, resolution 2.50Å" /> '''Structure of Porcine...
 
No edit summary
 
(15 intermediate revisions by the same user not shown)
Line 1: Line 1:
[[Image:2gmh.gif|left|200px]]<br /><applet load="2gmh" size="450" color="white" frame="true" align="right" spinBox="true"
caption="2gmh, resolution 2.50&Aring;" />
'''Structure of Porcine Electron Transfer Flavoprotein-Ubiquinone Oxidoreductase in Complexed with Ubiquinone'''<br />


==Overview==
==Structure of Porcine Electron Transfer Flavoprotein-Ubiquinone Oxidoreductase in Complexed with Ubiquinone==
Electron transfer flavoprotein-ubiquinone oxidoreductase (ETF-QO) is a, 4Fe4S flavoprotein located in the inner mitochondrial membrane. It, catalyzes ubiquinone (UQ) reduction by ETF, linking oxidation of fatty, acids and some amino acids to the mitochondrial respiratory chain., Deficiencies in ETF or ETF-QO result in multiple acyl-CoA dehydrogenase, deficiency, a human metabolic disease. Crystal structures of ETF-QO with, and without bound UQ were determined, and they are essentially identical., The molecule forms a single structural domain. Three functional regions, bind FAD, the 4Fe4S cluster, and UQ and are closely packed and share, structural elements, resulting in no discrete structural domains. The, UQ-binding pocket consists mainly of hydrophobic residues, and UQ binding, differs from that of other UQ-binding proteins. ETF-QO is a monotopic, integral membrane protein. The putative membrane-binding surface contains, an alpha-helix and a beta-hairpin, forming a hydrophobic plateau. The, UQ-flavin distance (8.5 A) is shorter than the UQ-cluster distance (18.8, A), and the very similar redox potentials of FAD and the cluster strongly, suggest that the flavin, not the cluster, transfers electrons to UQ. Two, possible electron transfer paths can be envisioned. First, electrons from, the ETF flavin semiquinone may enter the ETF-QO flavin one by one, followed by rapid equilibration with the cluster. Alternatively, electrons, may enter via the cluster, followed by equilibration between centers. In, both cases, when ETF-QO is reduced to a two-electron reduced state (one, electron at each redox center), the enzyme is primed to reduce UQ to, ubiquinol via FAD.
<StructureSection load='2gmh' size='340' side='right'caption='[[2gmh]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
 
== Structural highlights ==
==About this Structure==
<table><tr><td colspan='2'>[[2gmh]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GMH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2GMH FirstGlance]. <br>
2GMH is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa] with BHG, NA, SF4, FAD, UQ5 and EDO as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Electron-transferring-flavoprotein_dehydrogenase Electron-transferring-flavoprotein dehydrogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.5.5.1 1.5.5.1] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2GMH OCA].  
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5&#8491;</td></tr>
 
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BHG:2-HEXYLOXY-6-HYDROXYMETHYL-TETRAHYDRO-PYRAN-3,4,5-TRIOL'>BHG</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=SF4:IRON/SULFUR+CLUSTER'>SF4</scene>, <scene name='pdbligand=UQ5:2,3-DIMETHOXY-5-METHYL-6-(3,11,15,19-TETRAMETHYL-EICOSA-2,6,10,14,18-PENTAENYL)-[1,4]BENZOQUINONE'>UQ5</scene></td></tr>
==Reference==
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2gmh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2gmh OCA], [https://pdbe.org/2gmh PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2gmh RCSB], [https://www.ebi.ac.uk/pdbsum/2gmh PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2gmh ProSAT]</span></td></tr>
Structure of electron transfer flavoprotein-ubiquinone oxidoreductase and electron transfer to the mitochondrial ubiquinone pool., Zhang J, Frerman FE, Kim JJ, Proc Natl Acad Sci U S A. 2006 Oct 31;103(44):16212-7. Epub 2006 Oct 18. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17050691 17050691]
</table>
[[Category: Electron-transferring-flavoprotein dehydrogenase]]
== Function ==
[[Category: Single protein]]
[https://www.uniprot.org/uniprot/ETFD_PIG ETFD_PIG] Accepts electrons from ETF and reduces ubiquinone.
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/gm/2gmh_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2gmh ConSurf].
<div style="clear:both"></div>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Sus scrofa]]
[[Category: Sus scrofa]]
[[Category: Frerman, F.E.]]
[[Category: Frerman FE]]
[[Category: Kim, J.J.P.]]
[[Category: Kim J-JP]]
[[Category: Zhang, J.]]
[[Category: Zhang J]]
[[Category: BHG]]
[[Category: EDO]]
[[Category: FAD]]
[[Category: NA]]
[[Category: SF4]]
[[Category: UQ5]]
[[Category: electron-transfer]]
[[Category: flavoprotein]]
[[Category: oxidoreductase]]
[[Category: ubiquinone]]
 
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 11:16:40 2007''

Latest revision as of 12:28, 14 February 2024

Structure of Porcine Electron Transfer Flavoprotein-Ubiquinone Oxidoreductase in Complexed with UbiquinoneStructure of Porcine Electron Transfer Flavoprotein-Ubiquinone Oxidoreductase in Complexed with Ubiquinone

Structural highlights

2gmh is a 2 chain structure with sequence from Sus scrofa. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 2.5Å
Ligands:, , , , ,
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

ETFD_PIG Accepts electrons from ETF and reduces ubiquinone.

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

2gmh, resolution 2.50Å

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA
Retrieved from "https://proteopedia.openfox.io/wiki/index.php?title=2gmh&oldid=4058756"