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| [[Image:2gis.gif|left|200px]]<br /><applet load="2gis" size="350" color="white" frame="true" align="right" spinBox="true"
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| caption="2gis, resolution 2.90Å" />
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| '''Structure of the S-adenosylmethionine riboswitch mRNA regulatory element'''<br />
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| ==Overview== | | ==Structure of the S-adenosylmethionine riboswitch mRNA regulatory element== |
| Riboswitches are cis-acting genetic regulatory elements found in the 5'-untranslated regions of messenger RNAs that control gene expression through their ability to bind small molecule metabolites directly. Regulation occurs through the interplay of two domains of the RNA: an aptamer domain that responds to intracellular metabolite concentrations and an expression platform that uses two mutually exclusive secondary structures to direct a decision-making process. In Gram-positive bacteria such as Bacillus species, riboswitches control the expression of more than 2% of all genes through their ability to respond to a diverse set of metabolites including amino acids, nucleobases and protein cofactors. Here we report the 2.9-angstroms resolution crystal structure of an S-adenosylmethionine (SAM)-responsive riboswitch from Thermoanaerobacter tengcongensis complexed with S-adenosylmethionine, an RNA element that controls the expression of several genes involved in sulphur and methionine metabolism. This RNA folds into a complex three-dimensional architecture that recognizes almost every functional group of the ligand through a combination of direct and indirect readout mechanisms. Ligand binding induces the formation of a series of tertiary interactions with one of the helices, serving as a communication link between the aptamer and expression platform domains.
| | <StructureSection load='2gis' size='340' side='right'caption='[[2gis]], [[Resolution|resolution]] 2.90Å' scene=''> |
| | == Structural highlights == |
| | <table><tr><td colspan='2'>[[2gis]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Caldanaerobacter_subterraneus_subsp._tengcongensis Caldanaerobacter subterraneus subsp. tengcongensis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GIS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2GIS FirstGlance]. <br> |
| | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.9Å</td></tr> |
| | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=IRI:IRIDIUM+HEXAMMINE+ION'>IRI</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SAM:S-ADENOSYLMETHIONINE'>SAM</scene></td></tr> |
| | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2gis FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2gis OCA], [https://pdbe.org/2gis PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2gis RCSB], [https://www.ebi.ac.uk/pdbsum/2gis PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2gis ProSAT]</span></td></tr> |
| | </table> |
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| ==About this Structure== | | ==See Also== |
| 2GIS is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ] with <scene name='pdbligand=MG:'>MG</scene>, <scene name='pdbligand=IRI:'>IRI</scene> and <scene name='pdbligand=SAM:'>SAM</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GIS OCA].
| | *[[Kink-turn motif|Kink-turn motif]] |
| | | *[[Riboswitch 3D structures|Riboswitch 3D structures]] |
| ==Reference==
| | *[[User:Wayne Decatur/kink-turn motif|User:Wayne Decatur/kink-turn motif]] |
| Structure of the S-adenosylmethionine riboswitch regulatory mRNA element., Montange RK, Batey RT, Nature. 2006 Jun 29;441(7097):1172-5. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16810258 16810258]
| | __TOC__ |
| [[Category: Protein complex]]
| | </StructureSection> |
| [[Category: Batey, R T.]] | | [[Category: Caldanaerobacter subterraneus subsp. tengcongensis]] |
| [[Category: Montange, R K.]] | | [[Category: Large Structures]] |
| [[Category: IRI]]
| | [[Category: Batey RT]] |
| [[Category: MG]]
| | [[Category: Montange RK]] |
| [[Category: SAM]]
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| [[Category: mrna]] | |
| [[Category: riboswitch]] | |
| [[Category: rna-ligand complex]] | |
| [[Category: s-adenosylmethionine]] | |
| [[Category: sam]]
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| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:32:04 2008''
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