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[[Image:2gft.png|left|200px]]


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==Crystal structure of the E263A nucleophile mutant of Bacillus licheniformis endo-beta-1,4-galactanase in complex with galactotriose==
The line below this paragraph, containing "STRUCTURE_2gft", creates the "Structure Box" on the page.
<StructureSection load='2gft' size='340' side='right'caption='[[2gft]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
You may change the PDB parameter (which sets the PDB file loaded into the applet)
== Structural highlights ==
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
<table><tr><td colspan='2'>[[2gft]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_licheniformis Bacillus licheniformis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GFT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2GFT FirstGlance]. <br>
or leave the SCENE parameter empty for the default display.
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene>, <scene name='pdbligand=PRD_900114:4beta-beta-galactotriose'>PRD_900114</scene></td></tr>
{{STRUCTURE_2gft| PDB=2gft |  SCENE= }}
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2gft FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2gft OCA], [https://pdbe.org/2gft PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2gft RCSB], [https://www.ebi.ac.uk/pdbsum/2gft PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2gft ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/GANB_BACLD GANB_BACLD] Involved in galactan degradation (PubMed:15312766). Degrades arabinose-free galactan to galactooligosaccharides, producing galactotetraose as the main product along with galactotriose, galactobiose, and galactose (PubMed:15312766). May hydrolyze the beta-1,4-galactan linkages of the galactan portion of arabinogalactan type I, a pectic plant polysaccharide from which most of the arabinose has been removed (By similarity).[UniProtKB:O07013]<ref>PMID:15312766</ref>
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/gf/2gft_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2gft ConSurf].
<div style="clear:both"></div>


===Crystal structure of the E263A nucleophile mutant of Bacillus licheniformis endo-beta-1,4-galactanase in complex with galactotriose===
==See Also==
 
*[[Beta-1%2C4-galactanase|Beta-1%2C4-galactanase]]
 
== References ==
==About this Structure==
<references/>
2GFT is a 2 chains structure of sequences from [http://en.wikipedia.org/wiki/Bacillus_licheniformis Bacillus licheniformis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GFT OCA].
__TOC__
 
</StructureSection>
==Reference==
<ref group="xtra">PMID:15312766</ref><references group="xtra"/>
[[Category: Arabinogalactan endo-1,4-beta-galactosidase]]
[[Category: Bacillus licheniformis]]
[[Category: Bacillus licheniformis]]
[[Category: Christensen, L L.H.]]
[[Category: Large Structures]]
[[Category: Jorgensen, C T.]]
[[Category: Christensen LLH]]
[[Category: Larsen, S.]]
[[Category: De Maria L]]
[[Category: Leggio, L Lo.]]
[[Category: Jorgensen CT]]
[[Category: Maria, L De.]]
[[Category: Larsen S]]
[[Category: Nours, J Le.]]
[[Category: Le Nours J]]
[[Category: Welner, D]]
[[Category: Lo Leggio L]]
[[Category: Beta-alpha-8-barrel]]
[[Category: Welner D]]
[[Category: Nucleophile inactive mutant]]
[[Category: Protein-oligosaccharide complex]]
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Feb 17 13:38:12 2009''

Latest revision as of 12:27, 14 February 2024

Crystal structure of the E263A nucleophile mutant of Bacillus licheniformis endo-beta-1,4-galactanase in complex with galactotrioseCrystal structure of the E263A nucleophile mutant of Bacillus licheniformis endo-beta-1,4-galactanase in complex with galactotriose

Structural highlights

2gft is a 2 chain structure with sequence from Bacillus licheniformis. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 2.3Å
Ligands:, ,
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

GANB_BACLD Involved in galactan degradation (PubMed:15312766). Degrades arabinose-free galactan to galactooligosaccharides, producing galactotetraose as the main product along with galactotriose, galactobiose, and galactose (PubMed:15312766). May hydrolyze the beta-1,4-galactan linkages of the galactan portion of arabinogalactan type I, a pectic plant polysaccharide from which most of the arabinose has been removed (By similarity).[UniProtKB:O07013][1]

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

See Also

References

  1. ↑ Ryttersgaard C, Le Nours J, Lo Leggio L, Jorgensen CT, Christensen LL, Bjornvad M, Larsen S. The structure of endo-beta-1,4-galactanase from Bacillus licheniformis in complex with two oligosaccharide products. J Mol Biol. 2004 Jul 30;341(1):107-17. PMID:15312766 doi:http://dx.doi.org/10.1016/j.jmb.2004.05.017

2gft, resolution 2.30Å

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