2ge7: Difference between revisions

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OCA

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-[[Image:2ge7.gif|left|200px]]<br /><applet load="2ge7" size="450" color="white" frame="true" align="right" spinBox="true"
-caption="2ge7, resolution 2.000&Aring;" />
-'''Structure of the C-terminal dimerization domain of infectious bronchitis virus nucleocapsid protein'''<br />
-==Overview==
+==Structure of the C-terminal dimerization domain of infectious bronchitis virus nucleocapsid protein==
-Coronaviruses cause a variety of respiratory and enteric diseases in, animals and humans including severe acute respiratory syndrome. In these, enveloped viruses, the filamentous nucleocapsid is formed by the, association of nucleocapsid (N) protein with single-stranded viral RNA., The N protein is a highly immunogenic phosphoprotein also implicated in, viral genome replication and in modulating cell signaling pathways. We, describe the structure of the two proteolytically resistant domains of the, N protein from infectious bronchitis virus (IBV), a prototype coronavirus., These domains are located at its N- and C-terminal ends (NTD and CTD, respectively). The NTD of the IBV Gray strain at 1.3-A resolution exhibits, a U-shaped structure, with two arms rich in basic residues, providing a, module for specific interaction with RNA. The CTD forms a tightly, intertwined dimer with an intermolecular four-stranded central beta-sheet, platform flanked by alpha helices, indicating that the basic building, block for coronavirus nucleocapsid formation is a dimeric assembly of N, protein. The variety of quaternary arrangements of the NTD and CTD, revealed by the analysis of the different crystal forms delineates, possible interfaces that could be used for the formation of a flexible, filamentous ribonucleocapsid. The striking similarity between the dimeric, structure of CTD and the nucleocapsid-forming domain of a distantly, related arterivirus indicates a conserved mechanism of nucleocapsid, formation for these two viral families.
+<StructureSection load='2ge7' size='340' side='right'caption='[[2ge7]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[2ge7]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Infectious_bronchitis_virus Infectious bronchitis virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GE7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2GE7 FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2ge7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ge7 OCA], [https://pdbe.org/2ge7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2ge7 RCSB], [https://www.ebi.ac.uk/pdbsum/2ge7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2ge7 ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/NCAP_IBVG NCAP_IBVG] Major structural component of virions that associates with genomic RNA to form a long, flexible, helical nucleocapsid. Interaction with the M protein leads to the formation of virus particles. Binds to cellular membranes and phospholipids. Elicits cell-mediated immunity. May play roles in viral transcription and translation, and/or replication.
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ge/2ge7_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2ge7 ConSurf].
+<div style="clear:both"></div>
-==About this Structure==
+==See Also==
-GE7 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Infectious_bronchitis_virus Infectious bronchitis virus]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2GE7 OCA].
+*[[Nucleoprotein 3D structures|Nucleoprotein 3D structures]]
+__TOC__
-==Reference==
+</StructureSection>
-X-ray structures of the N- and C-terminal domains of a coronavirus nucleocapsid protein: implications for nucleocapsid formation., Jayaram H, Fan H, Bowman BR, Ooi A, Jayaram J, Collisson EW, Lescar J, Prasad BV, J Virol. 2006 Jul;80(13):6612-20. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16775348 16775348]
 [[Category: Infectious bronchitis virus]]
-[[Category: Single protein]]
+[[Category: Large Structures]]
-[[Category: Bowman, B.R.]]
+[[Category: Bowman BR]]
-[[Category: Collisson, E.W.]]
+[[Category: Collisson EW]]
-[[Category: Fan, H.]]
+[[Category: Fan H]]
-[[Category: Jayaram, H.]]
+[[Category: Jayaram H]]
-[[Category: Jayaram, J.]]
+[[Category: Jayaram J]]
-[[Category: Lescar, J.]]
+[[Category: Lescar J]]
-[[Category: Ooi, A.]]
+[[Category: Ooi A]]
-[[Category: Prasad, B.V.]]
+[[Category: Prasad BV]]
-[[Category: coronavirus]]
-[[Category: dimerization domain]]
-[[Category: ibv n protein]]
-[[Category: n protein]]
-[[Category: nucleocapsid protein]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 11:09:01 2007''